Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22260 and RR42_RS22265 are separated by 51 nucleotides RR42_RS22265 and RR42_RS22270 are separated by 1 nucleotides
RR42_RS22260: RR42_RS22260 - hypothetical protein, at 332,121 to 333,455
_RS22260
RR42_RS22265: RR42_RS22265 - hypothetical protein, at 333,507 to 337,334
_RS22265
RR42_RS22270: RR42_RS22270 - hypothetical protein, at 337,336 to 339,390
_RS22270
Position (kb) 333
334
335
336
337
338 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 332.932 kb on + strand, within RR42_RS22260 at 333.015 kb on + strand, within RR42_RS22260 at 333.015 kb on + strand, within RR42_RS22260 at 333.016 kb on - strand, within RR42_RS22260 at 333.047 kb on - strand, within RR42_RS22260 at 333.418 kb on + strand at 333.489 kb on + strand at 333.489 kb on + strand at 333.489 kb on + strand at 333.490 kb on - strand at 333.528 kb on - strand at 333.540 kb on - strand at 333.965 kb on - strand, within RR42_RS22265 at 333.965 kb on - strand, within RR42_RS22265 at 333.979 kb on + strand, within RR42_RS22265 at 333.979 kb on + strand, within RR42_RS22265 at 334.177 kb on + strand, within RR42_RS22265 at 334.309 kb on - strand, within RR42_RS22265 at 334.539 kb on - strand, within RR42_RS22265 at 334.747 kb on + strand, within RR42_RS22265 at 334.954 kb on - strand, within RR42_RS22265 at 335.675 kb on - strand, within RR42_RS22265 at 335.675 kb on - strand, within RR42_RS22265 at 335.684 kb on + strand, within RR42_RS22265 at 335.684 kb on + strand, within RR42_RS22265 at 335.685 kb on - strand, within RR42_RS22265 at 335.929 kb on + strand, within RR42_RS22265 at 335.929 kb on + strand, within RR42_RS22265 at 336.247 kb on + strand, within RR42_RS22265 at 336.418 kb on + strand, within RR42_RS22265 at 336.559 kb on + strand, within RR42_RS22265 at 336.785 kb on - strand, within RR42_RS22265 at 336.785 kb on - strand, within RR42_RS22265 at 336.785 kb on - strand, within RR42_RS22265 at 336.785 kb on - strand, within RR42_RS22265 at 337.054 kb on + strand at 337.054 kb on + strand at 337.072 kb on + strand at 337.177 kb on + strand at 337.282 kb on + strand at 337.391 kb on + strand at 337.391 kb on + strand at 337.392 kb on - strand at 337.392 kb on - strand at 337.421 kb on + strand at 337.421 kb on + strand at 337.497 kb on - strand at 337.892 kb on + strand, within RR42_RS22270 at 338.177 kb on + strand, within RR42_RS22270 at 338.177 kb on + strand, within RR42_RS22270 at 338.178 kb on - strand, within RR42_RS22270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 332,932 + RR42_RS22260 0.61 +1.1 333,015 + RR42_RS22260 0.67 -0.5 333,015 + RR42_RS22260 0.67 -0.1 333,016 - RR42_RS22260 0.67 -2.0 333,047 - RR42_RS22260 0.69 -0.3 333,418 + -2.8 333,489 + -2.3 333,489 + -0.2 333,489 + +0.4 333,490 - +0.3 333,528 - -0.4 333,540 - +1.0 333,965 - RR42_RS22265 0.12 -0.3 333,965 - RR42_RS22265 0.12 -0.2 333,979 + RR42_RS22265 0.12 -2.2 333,979 + RR42_RS22265 0.12 -0.3 334,177 + RR42_RS22265 0.18 -2.5 334,309 - RR42_RS22265 0.21 +0.0 334,539 - RR42_RS22265 0.27 +0.4 334,747 + RR42_RS22265 0.32 -0.8 334,954 - RR42_RS22265 0.38 -0.5 335,675 - RR42_RS22265 0.57 -1.1 335,675 - RR42_RS22265 0.57 +0.5 335,684 + RR42_RS22265 0.57 -1.5 335,684 + RR42_RS22265 0.57 -0.2 335,685 - RR42_RS22265 0.57 -2.0 335,929 + RR42_RS22265 0.63 -2.8 335,929 + RR42_RS22265 0.63 -0.2 336,247 + RR42_RS22265 0.72 +0.5 336,418 + RR42_RS22265 0.76 -0.0 336,559 + RR42_RS22265 0.80 +1.2 336,785 - RR42_RS22265 0.86 +1.3 336,785 - RR42_RS22265 0.86 -0.6 336,785 - RR42_RS22265 0.86 +1.2 336,785 - RR42_RS22265 0.86 +0.7 337,054 + +1.0 337,054 + -2.1 337,072 + -0.3 337,177 + -1.0 337,282 + +0.3 337,391 + -1.8 337,391 + +0.2 337,392 - +0.6 337,392 - +0.8 337,421 + +1.5 337,421 + +0.2 337,497 - +1.2 337,892 + RR42_RS22270 0.27 -0.7 338,177 + RR42_RS22270 0.41 -3.3 338,177 + RR42_RS22270 0.41 +0.3 338,178 - RR42_RS22270 0.41 +0.5
Or see this region's nucleotide sequence