Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22245 and RR42_RS22250 are separated by 25 nucleotides RR42_RS22250 and RR42_RS22255 are separated by 23 nucleotides RR42_RS22255 and RR42_RS22260 are separated by 24 nucleotides
RR42_RS22245: RR42_RS22245 - cytochrome C biogenesis protein, at 329,373 to 330,320
_RS22245
RR42_RS22250: RR42_RS22250 - hypothetical protein, at 330,346 to 330,642
_RS22250
RR42_RS22255: RR42_RS22255 - hypothetical protein, at 330,666 to 332,096
_RS22255
RR42_RS22260: RR42_RS22260 - hypothetical protein, at 332,121 to 333,455
_RS22260
Position (kb)
330
331
332
333 Strain fitness (log2 ratio)
-2
-1
0
1 at 329.834 kb on + strand, within RR42_RS22245 at 329.834 kb on + strand, within RR42_RS22245 at 329.983 kb on - strand, within RR42_RS22245 at 330.284 kb on + strand at 330.284 kb on + strand at 330.341 kb on + strand at 330.342 kb on - strand at 330.609 kb on - strand, within RR42_RS22250 at 330.718 kb on + strand at 330.718 kb on + strand at 330.718 kb on + strand at 330.718 kb on + strand at 330.719 kb on - strand at 330.719 kb on - strand at 330.719 kb on - strand at 330.947 kb on - strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.333 kb on - strand, within RR42_RS22255 at 331.333 kb on - strand, within RR42_RS22255 at 331.544 kb on - strand, within RR42_RS22255 at 331.544 kb on - strand, within RR42_RS22255 at 331.564 kb on + strand, within RR42_RS22255 at 331.565 kb on - strand, within RR42_RS22255 at 331.565 kb on - strand, within RR42_RS22255 at 331.631 kb on - strand, within RR42_RS22255 at 331.678 kb on + strand, within RR42_RS22255 at 331.678 kb on + strand, within RR42_RS22255 at 331.679 kb on - strand, within RR42_RS22255 at 331.681 kb on + strand, within RR42_RS22255 at 331.682 kb on - strand, within RR42_RS22255 at 331.785 kb on + strand, within RR42_RS22255 at 331.785 kb on + strand, within RR42_RS22255 at 331.933 kb on + strand, within RR42_RS22255 at 332.068 kb on - strand at 332.212 kb on + strand at 332.257 kb on + strand, within RR42_RS22260 at 332.257 kb on + strand, within RR42_RS22260 at 332.257 kb on + strand, within RR42_RS22260 at 332.258 kb on - strand, within RR42_RS22260 at 332.258 kb on - strand, within RR42_RS22260 at 332.345 kb on - strand, within RR42_RS22260 at 332.932 kb on + strand, within RR42_RS22260 at 333.015 kb on + strand, within RR42_RS22260 at 333.015 kb on + strand, within RR42_RS22260 at 333.016 kb on - strand, within RR42_RS22260 at 333.047 kb on - strand, within RR42_RS22260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 329,834 + RR42_RS22245 0.49 -0.6 329,834 + RR42_RS22245 0.49 -0.1 329,983 - RR42_RS22245 0.64 -0.5 330,284 + -0.6 330,284 + +0.4 330,341 + -0.7 330,342 - +1.4 330,609 - RR42_RS22250 0.89 -2.0 330,718 + -0.5 330,718 + +0.7 330,718 + -1.8 330,718 + +0.3 330,719 - -1.6 330,719 - -1.3 330,719 - +0.6 330,947 - RR42_RS22255 0.20 +1.0 331,332 + RR42_RS22255 0.47 +0.0 331,332 + RR42_RS22255 0.47 +0.4 331,332 + RR42_RS22255 0.47 +0.5 331,333 - RR42_RS22255 0.47 +0.7 331,333 - RR42_RS22255 0.47 +0.3 331,544 - RR42_RS22255 0.61 +0.2 331,544 - RR42_RS22255 0.61 +0.8 331,564 + RR42_RS22255 0.63 -0.1 331,565 - RR42_RS22255 0.63 -0.8 331,565 - RR42_RS22255 0.63 -0.4 331,631 - RR42_RS22255 0.67 -0.2 331,678 + RR42_RS22255 0.71 -1.3 331,678 + RR42_RS22255 0.71 -0.5 331,679 - RR42_RS22255 0.71 -0.0 331,681 + RR42_RS22255 0.71 +1.1 331,682 - RR42_RS22255 0.71 -2.1 331,785 + RR42_RS22255 0.78 -0.8 331,785 + RR42_RS22255 0.78 +0.5 331,933 + RR42_RS22255 0.89 -0.9 332,068 - -0.4 332,212 + +0.2 332,257 + RR42_RS22260 0.10 -0.9 332,257 + RR42_RS22260 0.10 +0.1 332,257 + RR42_RS22260 0.10 +1.0 332,258 - RR42_RS22260 0.10 -0.5 332,258 - RR42_RS22260 0.10 -1.5 332,345 - RR42_RS22260 0.17 -0.7 332,932 + RR42_RS22260 0.61 +1.1 333,015 + RR42_RS22260 0.67 -0.5 333,015 + RR42_RS22260 0.67 -0.1 333,016 - RR42_RS22260 0.67 -2.0 333,047 - RR42_RS22260 0.69 -0.3
Or see this region's nucleotide sequence