Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22010 and RR42_RS22015 are separated by 25 nucleotides RR42_RS22015 and RR42_RS22020 are separated by 15 nucleotides RR42_RS22020 and RR42_RS22025 are separated by 256 nucleotides
RR42_RS22010: RR42_RS22010 - histidine kinase, at 269,587 to 271,233
_RS22010
RR42_RS22015: RR42_RS22015 - histidine kinase, at 271,259 to 273,655
_RS22015
RR42_RS22020: RR42_RS22020 - hypothetical protein, at 273,671 to 273,856
_RS22020
RR42_RS22025: RR42_RS22025 - AraC family transcriptional regulator, at 274,113 to 275,090
_RS22025
Position (kb) 271
272
273
274 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 270.275 kb on + strand, within RR42_RS22010 at 270.535 kb on + strand, within RR42_RS22010 at 270.535 kb on + strand, within RR42_RS22010 at 270.535 kb on + strand, within RR42_RS22010 at 270.628 kb on - strand, within RR42_RS22010 at 270.628 kb on - strand, within RR42_RS22010 at 270.710 kb on - strand, within RR42_RS22010 at 270.988 kb on + strand, within RR42_RS22010 at 271.020 kb on + strand, within RR42_RS22010 at 271.090 kb on + strand at 271.091 kb on - strand at 271.168 kb on + strand at 271.168 kb on + strand at 271.168 kb on + strand at 271.231 kb on + strand at 272.790 kb on - strand, within RR42_RS22015 at 272.802 kb on + strand, within RR42_RS22015 at 272.803 kb on - strand, within RR42_RS22015 at 272.803 kb on - strand, within RR42_RS22015 at 272.960 kb on - strand, within RR42_RS22015 at 273.244 kb on - strand, within RR42_RS22015 at 273.244 kb on - strand, within RR42_RS22015 at 273.414 kb on - strand, within RR42_RS22015 at 273.846 kb on + strand at 273.847 kb on - strand at 273.902 kb on + strand at 273.916 kb on - strand at 273.936 kb on - strand at 274.017 kb on + strand at 274.018 kb on - strand at 274.018 kb on - strand at 274.162 kb on + strand at 274.275 kb on - strand, within RR42_RS22025 at 274.275 kb on - strand, within RR42_RS22025 at 274.279 kb on - strand, within RR42_RS22025 at 274.654 kb on + strand, within RR42_RS22025 at 274.654 kb on + strand, within RR42_RS22025 at 274.655 kb on - strand, within RR42_RS22025 at 274.655 kb on - strand, within RR42_RS22025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 270,275 + RR42_RS22010 0.42 -1.3 270,535 + RR42_RS22010 0.58 +0.4 270,535 + RR42_RS22010 0.58 +1.5 270,535 + RR42_RS22010 0.58 +0.5 270,628 - RR42_RS22010 0.63 -0.4 270,628 - RR42_RS22010 0.63 -0.5 270,710 - RR42_RS22010 0.68 +1.1 270,988 + RR42_RS22010 0.85 +0.2 271,020 + RR42_RS22010 0.87 -1.8 271,090 + +0.2 271,091 - -3.2 271,168 + +0.3 271,168 + +2.3 271,168 + +0.2 271,231 + -0.5 272,790 - RR42_RS22015 0.64 +1.2 272,802 + RR42_RS22015 0.64 +0.3 272,803 - RR42_RS22015 0.64 -1.9 272,803 - RR42_RS22015 0.64 +0.9 272,960 - RR42_RS22015 0.71 -2.4 273,244 - RR42_RS22015 0.83 +1.1 273,244 - RR42_RS22015 0.83 +1.6 273,414 - RR42_RS22015 0.90 -2.2 273,846 + +1.0 273,847 - +0.7 273,902 + +0.0 273,916 - +0.8 273,936 - +0.1 274,017 + +0.9 274,018 - +1.2 274,018 - -2.8 274,162 + -0.5 274,275 - RR42_RS22025 0.17 +1.2 274,275 - RR42_RS22025 0.17 -0.6 274,279 - RR42_RS22025 0.17 +0.8 274,654 + RR42_RS22025 0.55 +1.0 274,654 + RR42_RS22025 0.55 -0.5 274,655 - RR42_RS22025 0.55 -0.2 274,655 - RR42_RS22025 0.55 +0.1
Or see this region's nucleotide sequence