Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21580 and RR42_RS21585 are separated by 45 nucleotides RR42_RS21585 and RR42_RS21590 are separated by 457 nucleotides
RR42_RS21580: RR42_RS21580 - cysteine desulfurase, at 167,020 to 168,204
_RS21580
RR42_RS21585: RR42_RS21585 - membrane protein, at 168,250 to 169,857
_RS21585
RR42_RS21590: RR42_RS21590 - hypothetical protein, at 170,315 to 173,065
_RS21590
Position (kb)
168
169
170 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 167.264 kb on + strand, within RR42_RS21580 at 167.468 kb on + strand, within RR42_RS21580 at 167.468 kb on + strand, within RR42_RS21580 at 167.469 kb on - strand, within RR42_RS21580 at 167.469 kb on - strand, within RR42_RS21580 at 167.531 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.944 kb on - strand, within RR42_RS21580 at 167.944 kb on - strand, within RR42_RS21580 at 168.035 kb on + strand, within RR42_RS21580 at 168.035 kb on + strand, within RR42_RS21580 at 168.036 kb on - strand, within RR42_RS21580 at 168.036 kb on - strand, within RR42_RS21580 at 168.036 kb on - strand, within RR42_RS21580 at 168.062 kb on + strand, within RR42_RS21580 at 168.152 kb on + strand at 168.153 kb on - strand at 168.153 kb on - strand at 168.153 kb on - strand at 168.153 kb on - strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.394 kb on + strand at 168.561 kb on - strand, within RR42_RS21585 at 168.722 kb on + strand, within RR42_RS21585 at 168.740 kb on + strand, within RR42_RS21585 at 168.915 kb on - strand, within RR42_RS21585 at 168.932 kb on + strand, within RR42_RS21585 at 168.966 kb on + strand, within RR42_RS21585 at 168.967 kb on - strand, within RR42_RS21585 at 169.017 kb on - strand, within RR42_RS21585 at 169.173 kb on + strand, within RR42_RS21585 at 169.175 kb on + strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.455 kb on - strand, within RR42_RS21585 at 169.535 kb on + strand, within RR42_RS21585 at 169.863 kb on + strand at 169.864 kb on - strand at 169.984 kb on + strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 170.141 kb on + strand at 170.142 kb on - strand at 170.236 kb on + strand at 170.237 kb on - strand at 170.237 kb on - strand at 170.238 kb on + strand at 170.238 kb on + strand at 170.238 kb on + strand at 170.238 kb on + strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.464 kb on + strand at 170.464 kb on + strand at 170.579 kb on + strand at 170.606 kb on + strand, within RR42_RS21590 at 170.690 kb on + strand, within RR42_RS21590 at 170.742 kb on - strand, within RR42_RS21590 at 170.829 kb on - strand, within RR42_RS21590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 167,264 + RR42_RS21580 0.21 +0.9 167,468 + RR42_RS21580 0.38 -0.1 167,468 + RR42_RS21580 0.38 -0.7 167,469 - RR42_RS21580 0.38 +0.3 167,469 - RR42_RS21580 0.38 -2.0 167,531 + RR42_RS21580 0.43 +0.0 167,943 + RR42_RS21580 0.78 -1.3 167,943 + RR42_RS21580 0.78 -1.0 167,943 + RR42_RS21580 0.78 -0.2 167,944 - RR42_RS21580 0.78 -0.1 167,944 - RR42_RS21580 0.78 -1.0 168,035 + RR42_RS21580 0.86 -0.5 168,035 + RR42_RS21580 0.86 +0.3 168,036 - RR42_RS21580 0.86 +0.5 168,036 - RR42_RS21580 0.86 +0.2 168,036 - RR42_RS21580 0.86 -0.5 168,062 + RR42_RS21580 0.88 -1.3 168,152 + +0.3 168,153 - -1.3 168,153 - -0.1 168,153 - +0.9 168,153 - +1.8 168,251 + +0.0 168,251 + +0.1 168,251 + -2.5 168,251 + +1.0 168,251 + -1.3 168,251 + -0.5 168,394 + +0.4 168,561 - RR42_RS21585 0.19 -2.4 168,722 + RR42_RS21585 0.29 +0.3 168,740 + RR42_RS21585 0.30 -3.0 168,915 - RR42_RS21585 0.41 -0.6 168,932 + RR42_RS21585 0.42 -0.4 168,966 + RR42_RS21585 0.45 +0.9 168,967 - RR42_RS21585 0.45 +1.0 169,017 - RR42_RS21585 0.48 +0.1 169,173 + RR42_RS21585 0.57 +0.1 169,175 + RR42_RS21585 0.58 -2.0 169,176 - RR42_RS21585 0.58 -0.1 169,176 - RR42_RS21585 0.58 -1.7 169,176 - RR42_RS21585 0.58 -0.7 169,176 - RR42_RS21585 0.58 -3.3 169,176 - RR42_RS21585 0.58 -0.2 169,454 + RR42_RS21585 0.75 +1.6 169,454 + RR42_RS21585 0.75 -1.9 169,454 + RR42_RS21585 0.75 +1.5 169,454 + RR42_RS21585 0.75 -0.7 169,454 + RR42_RS21585 0.75 -1.5 169,454 + RR42_RS21585 0.75 +0.3 169,455 - RR42_RS21585 0.75 +0.9 169,535 + RR42_RS21585 0.80 -1.2 169,863 + -2.2 169,864 - -0.3 169,984 + -0.2 169,985 - -2.7 169,985 - -1.9 169,985 - +0.3 169,985 - -1.0 169,985 - -0.3 169,985 - -0.7 169,985 - -1.3 169,985 - -1.1 169,985 - -1.1 169,985 - +0.1 169,985 - -0.0 170,141 + -0.3 170,142 - +0.2 170,236 + -1.7 170,237 - +0.5 170,237 - -0.5 170,238 + -0.0 170,238 + -2.4 170,238 + +1.9 170,238 + +0.3 170,239 - -0.2 170,239 - -0.7 170,239 - -0.6 170,239 - -1.0 170,239 - -1.9 170,239 - +0.5 170,239 - +2.3 170,239 - -3.0 170,239 - -0.1 170,239 - +1.3 170,239 - -2.0 170,239 - -1.5 170,464 + +0.3 170,464 + -0.2 170,579 + -0.5 170,606 + RR42_RS21590 0.11 +3.3 170,690 + RR42_RS21590 0.14 -1.6 170,742 - RR42_RS21590 0.16 -1.0 170,829 - RR42_RS21590 0.19 -0.1
Or see this region's nucleotide sequence