Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21555 and RR42_RS21560 are separated by 11 nucleotides RR42_RS21560 and RR42_RS21565 are separated by 120 nucleotides
RR42_RS21555: RR42_RS21555 - membrane protein, at 161,530 to 162,519
_RS21555
RR42_RS21560: RR42_RS21560 - hypothetical protein, at 162,531 to 163,229
_RS21560
RR42_RS21565: RR42_RS21565 - hypothetical protein, at 163,350 to 164,309
_RS21565
Position (kb) 162
163
164 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 161.734 kb on + strand, within RR42_RS21555 at 161.735 kb on - strand, within RR42_RS21555 at 161.831 kb on - strand, within RR42_RS21555 at 161.831 kb on - strand, within RR42_RS21555 at 161.944 kb on + strand, within RR42_RS21555 at 161.944 kb on + strand, within RR42_RS21555 at 162.043 kb on + strand, within RR42_RS21555 at 162.043 kb on + strand, within RR42_RS21555 at 162.043 kb on + strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.278 kb on + strand, within RR42_RS21555 at 162.316 kb on + strand, within RR42_RS21555 at 162.338 kb on + strand, within RR42_RS21555 at 162.387 kb on + strand, within RR42_RS21555 at 162.548 kb on - strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.616 kb on + strand, within RR42_RS21560 at 162.617 kb on - strand, within RR42_RS21560 at 162.678 kb on + strand, within RR42_RS21560 at 162.761 kb on + strand, within RR42_RS21560 at 162.871 kb on + strand, within RR42_RS21560 at 162.872 kb on - strand, within RR42_RS21560 at 163.240 kb on + strand at 163.278 kb on + strand at 163.278 kb on + strand at 163.278 kb on + strand at 163.279 kb on - strand at 163.279 kb on - strand at 163.279 kb on - strand at 163.279 kb on - strand at 163.393 kb on + strand at 163.729 kb on + strand, within RR42_RS21565 at 163.729 kb on + strand, within RR42_RS21565 at 163.730 kb on - strand, within RR42_RS21565 at 163.730 kb on - strand, within RR42_RS21565 at 163.730 kb on - strand, within RR42_RS21565 at 163.730 kb on - strand, within RR42_RS21565 at 163.837 kb on + strand, within RR42_RS21565 at 163.838 kb on - strand, within RR42_RS21565 at 163.838 kb on - strand, within RR42_RS21565 at 164.034 kb on + strand, within RR42_RS21565 at 164.221 kb on + strand at 164.222 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 161,734 + RR42_RS21555 0.21 -1.3 161,735 - RR42_RS21555 0.21 +0.4 161,831 - RR42_RS21555 0.30 +0.2 161,831 - RR42_RS21555 0.30 +0.8 161,944 + RR42_RS21555 0.42 +0.7 161,944 + RR42_RS21555 0.42 -1.0 162,043 + RR42_RS21555 0.52 -1.4 162,043 + RR42_RS21555 0.52 +0.6 162,043 + RR42_RS21555 0.52 +0.8 162,044 - RR42_RS21555 0.52 -2.5 162,044 - RR42_RS21555 0.52 +0.6 162,044 - RR42_RS21555 0.52 -0.9 162,044 - RR42_RS21555 0.52 +0.7 162,044 - RR42_RS21555 0.52 +1.4 162,278 + RR42_RS21555 0.76 +0.6 162,316 + RR42_RS21555 0.79 +0.8 162,338 + RR42_RS21555 0.82 -0.3 162,387 + RR42_RS21555 0.87 +0.4 162,548 - +0.2 162,580 + +0.9 162,580 + +0.1 162,580 + +0.7 162,580 + +1.3 162,580 + -1.0 162,581 - +1.4 162,581 - -2.3 162,581 - +0.4 162,581 - +0.3 162,581 - +1.2 162,616 + RR42_RS21560 0.12 +0.5 162,617 - RR42_RS21560 0.12 -0.1 162,678 + RR42_RS21560 0.21 -1.1 162,761 + RR42_RS21560 0.33 +0.9 162,871 + RR42_RS21560 0.49 +1.3 162,872 - RR42_RS21560 0.49 +1.0 163,240 + -0.0 163,278 + -3.4 163,278 + -0.6 163,278 + -0.2 163,279 - +0.5 163,279 - -0.7 163,279 - -0.6 163,279 - +0.7 163,393 + -1.1 163,729 + RR42_RS21565 0.39 -0.0 163,729 + RR42_RS21565 0.39 +0.5 163,730 - RR42_RS21565 0.40 -3.9 163,730 - RR42_RS21565 0.40 -1.3 163,730 - RR42_RS21565 0.40 +1.1 163,730 - RR42_RS21565 0.40 +0.1 163,837 + RR42_RS21565 0.51 -2.6 163,838 - RR42_RS21565 0.51 -0.9 163,838 - RR42_RS21565 0.51 -1.7 164,034 + RR42_RS21565 0.71 -0.4 164,221 + +0.8 164,222 - +0.1
Or see this region's nucleotide sequence