Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21350 and RR42_RS21355 are separated by 79 nucleotides RR42_RS21355 and RR42_RS21360 overlap by 4 nucleotides RR42_RS21360 and RR42_RS21365 are separated by 19 nucleotides
RR42_RS21350: RR42_RS21350 - peptide ABC transporter substrate-binding protein, at 118,746 to 120,308
_RS21350
RR42_RS21355: RR42_RS21355 - ABC transporter permease, at 120,388 to 121,392
_RS21355
RR42_RS21360: RR42_RS21360 - ABC transporter permease, at 121,389 to 122,228
_RS21360
RR42_RS21365: RR42_RS21365 - ABC transporter ATP-binding protein, at 122,248 to 123,885
_RS21365
Position (kb) 120
121
122 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 119.413 kb on - strand, within RR42_RS21350 at 119.413 kb on - strand, within RR42_RS21350 at 119.811 kb on + strand, within RR42_RS21350 at 119.839 kb on - strand, within RR42_RS21350 at 119.869 kb on - strand, within RR42_RS21350 at 119.979 kb on + strand, within RR42_RS21350 at 119.980 kb on - strand, within RR42_RS21350 at 119.985 kb on + strand, within RR42_RS21350 at 119.986 kb on - strand, within RR42_RS21350 at 120.025 kb on - strand, within RR42_RS21350 at 120.025 kb on - strand, within RR42_RS21350 at 120.150 kb on + strand, within RR42_RS21350 at 120.151 kb on - strand, within RR42_RS21350 at 120.311 kb on - strand at 120.311 kb on - strand at 120.326 kb on + strand at 120.327 kb on - strand at 120.640 kb on + strand, within RR42_RS21355 at 120.830 kb on - strand, within RR42_RS21355 at 121.016 kb on - strand, within RR42_RS21355 at 121.016 kb on - strand, within RR42_RS21355 at 121.150 kb on - strand, within RR42_RS21355 at 121.411 kb on - strand at 121.803 kb on + strand, within RR42_RS21360 at 121.804 kb on - strand, within RR42_RS21360 at 122.268 kb on - strand at 122.348 kb on - strand at 122.348 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 119,413 - RR42_RS21350 0.43 -0.5 119,413 - RR42_RS21350 0.43 +0.6 119,811 + RR42_RS21350 0.68 +0.9 119,839 - RR42_RS21350 0.70 +0.9 119,869 - RR42_RS21350 0.72 -0.7 119,979 + RR42_RS21350 0.79 -2.0 119,980 - RR42_RS21350 0.79 -1.2 119,985 + RR42_RS21350 0.79 -2.6 119,986 - RR42_RS21350 0.79 -0.4 120,025 - RR42_RS21350 0.82 -0.8 120,025 - RR42_RS21350 0.82 -0.7 120,150 + RR42_RS21350 0.90 -3.0 120,151 - RR42_RS21350 0.90 +0.8 120,311 - -0.2 120,311 - -0.5 120,326 + -0.7 120,327 - +0.3 120,640 + RR42_RS21355 0.25 +0.6 120,830 - RR42_RS21355 0.44 +0.3 121,016 - RR42_RS21355 0.62 -2.3 121,016 - RR42_RS21355 0.62 -0.2 121,150 - RR42_RS21355 0.76 -1.9 121,411 - +0.1 121,803 + RR42_RS21360 0.49 +0.9 121,804 - RR42_RS21360 0.49 +0.5 122,268 - -2.0 122,348 - +0.2 122,348 - +1.2
Or see this region's nucleotide sequence