Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21285 and RR42_RS21290 are separated by 35 nucleotides RR42_RS21290 and RR42_RS21295 overlap by 4 nucleotides RR42_RS21295 and RR42_RS21300 are separated by 138 nucleotides
RR42_RS21285: RR42_RS21285 - L-lactate dehydrogenase, LutB subunit <small>(from data)</small>, at 102,111 to 103,541
_RS21285
RR42_RS21290: RR42_RS21290 - L-lactate dehydrogenase, LutC subunit <small>(from data)</small>, at 103,577 to 104,281
_RS21290
RR42_RS21295: RR42_RS21295 - L-lactate dehydrogenase, LutA subunit <small>(from data)</small>, at 104,278 to 105,066
_RS21295
RR42_RS21300: RR42_RS21300 - GntR family transcriptional regulator, at 105,205 to 105,930
_RS21300
Position (kb) 104
105
106 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 103.574 kb on + strand at 103.574 kb on + strand at 103.575 kb on - strand at 103.575 kb on - strand at 103.616 kb on - strand at 103.695 kb on - strand, within RR42_RS21290 at 103.695 kb on - strand, within RR42_RS21290 at 103.779 kb on + strand, within RR42_RS21290 at 103.889 kb on - strand, within RR42_RS21290 at 104.175 kb on + strand, within RR42_RS21290 at 104.175 kb on + strand, within RR42_RS21290 at 104.175 kb on + strand, within RR42_RS21290 at 104.176 kb on - strand, within RR42_RS21290 at 104.240 kb on + strand at 104.711 kb on + strand, within RR42_RS21295 at 104.895 kb on + strand, within RR42_RS21295 at 104.896 kb on - strand, within RR42_RS21295 at 105.026 kb on + strand at 105.130 kb on + strand at 105.331 kb on + strand, within RR42_RS21300 at 105.331 kb on + strand, within RR42_RS21300 at 105.332 kb on - strand, within RR42_RS21300 at 105.711 kb on + strand, within RR42_RS21300 at 105.711 kb on + strand, within RR42_RS21300 at 105.712 kb on - strand, within RR42_RS21300 at 105.794 kb on + strand, within RR42_RS21300 at 105.795 kb on - strand, within RR42_RS21300 at 105.795 kb on - strand, within RR42_RS21300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 103,574 + +0.3 103,574 + -0.7 103,575 - -1.3 103,575 - -1.8 103,616 - -1.3 103,695 - RR42_RS21290 0.17 +0.2 103,695 - RR42_RS21290 0.17 -1.4 103,779 + RR42_RS21290 0.29 -2.2 103,889 - RR42_RS21290 0.44 -1.3 104,175 + RR42_RS21290 0.85 +0.3 104,175 + RR42_RS21290 0.85 -1.5 104,175 + RR42_RS21290 0.85 -0.3 104,176 - RR42_RS21290 0.85 -0.5 104,240 + +1.1 104,711 + RR42_RS21295 0.55 -2.3 104,895 + RR42_RS21295 0.78 -0.5 104,896 - RR42_RS21295 0.78 -2.1 105,026 + +0.1 105,130 + +0.5 105,331 + RR42_RS21300 0.17 -0.6 105,331 + RR42_RS21300 0.17 -2.8 105,332 - RR42_RS21300 0.17 +0.0 105,711 + RR42_RS21300 0.70 -1.0 105,711 + RR42_RS21300 0.70 +1.3 105,712 - RR42_RS21300 0.70 +0.2 105,794 + RR42_RS21300 0.81 -2.2 105,795 - RR42_RS21300 0.81 -0.2 105,795 - RR42_RS21300 0.81 +0.5
Or see this region's nucleotide sequence