Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21230 and RR42_RS21235 are separated by 70 nucleotides RR42_RS21235 and RR42_RS21240 are separated by 571 nucleotides
RR42_RS21230: RR42_RS21230 - ABC transporter substrate-binding protein, at 88,047 to 89,039
_RS21230
RR42_RS21235: RR42_RS21235 - thymidine phosphorylase, at 89,110 to 91,149
_RS21235
RR42_RS21240: RR42_RS21240 - MarR family transcriptional regulator, at 91,721 to 92,266
_RS21240
Position (kb) 89
90
91
92 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 88.282 kb on + strand, within RR42_RS21230 at 88.283 kb on - strand, within RR42_RS21230 at 88.384 kb on + strand, within RR42_RS21230 at 88.384 kb on + strand, within RR42_RS21230 at 88.384 kb on + strand, within RR42_RS21230 at 88.385 kb on - strand, within RR42_RS21230 at 88.468 kb on + strand, within RR42_RS21230 at 88.468 kb on + strand, within RR42_RS21230 at 88.555 kb on + strand, within RR42_RS21230 at 88.556 kb on - strand, within RR42_RS21230 at 88.556 kb on - strand, within RR42_RS21230 at 88.556 kb on - strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.752 kb on - strand, within RR42_RS21230 at 88.752 kb on - strand, within RR42_RS21230 at 88.752 kb on - strand, within RR42_RS21230 at 88.752 kb on - strand, within RR42_RS21230 at 88.753 kb on + strand, within RR42_RS21230 at 88.754 kb on - strand, within RR42_RS21230 at 88.780 kb on + strand, within RR42_RS21230 at 88.780 kb on + strand, within RR42_RS21230 at 88.781 kb on - strand, within RR42_RS21230 at 88.794 kb on - strand, within RR42_RS21230 at 89.019 kb on + strand at 89.019 kb on + strand at 89.068 kb on + strand at 89.068 kb on + strand at 89.068 kb on + strand at 89.069 kb on - strand at 89.069 kb on - strand at 89.111 kb on + strand at 89.112 kb on - strand at 89.584 kb on + strand, within RR42_RS21235 at 89.584 kb on + strand, within RR42_RS21235 at 89.585 kb on - strand, within RR42_RS21235 at 89.585 kb on - strand, within RR42_RS21235 at 90.249 kb on + strand, within RR42_RS21235 at 90.250 kb on - strand, within RR42_RS21235 at 90.250 kb on - strand, within RR42_RS21235 at 90.288 kb on - strand, within RR42_RS21235 at 90.376 kb on - strand, within RR42_RS21235 at 90.542 kb on + strand, within RR42_RS21235 at 90.542 kb on + strand, within RR42_RS21235 at 90.542 kb on + strand, within RR42_RS21235 at 90.542 kb on + strand, within RR42_RS21235 at 90.542 kb on + strand, within RR42_RS21235 at 90.543 kb on - strand, within RR42_RS21235 at 90.543 kb on - strand, within RR42_RS21235 at 90.543 kb on - strand, within RR42_RS21235 at 90.909 kb on - strand, within RR42_RS21235 at 91.042 kb on + strand at 91.042 kb on + strand at 91.042 kb on + strand at 91.043 kb on - strand at 91.046 kb on + strand at 91.046 kb on + strand at 91.046 kb on + strand at 91.047 kb on - strand at 91.336 kb on + strand at 91.383 kb on + strand at 91.635 kb on + strand at 91.636 kb on - strand at 91.636 kb on - strand at 91.636 kb on - strand at 91.720 kb on + strand at 91.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 88,282 + RR42_RS21230 0.24 +1.1 88,283 - RR42_RS21230 0.24 -2.4 88,384 + RR42_RS21230 0.34 +0.5 88,384 + RR42_RS21230 0.34 -1.2 88,384 + RR42_RS21230 0.34 +0.5 88,385 - RR42_RS21230 0.34 +0.1 88,468 + RR42_RS21230 0.42 +1.0 88,468 + RR42_RS21230 0.42 +0.4 88,555 + RR42_RS21230 0.51 +0.5 88,556 - RR42_RS21230 0.51 +0.4 88,556 - RR42_RS21230 0.51 -0.2 88,556 - RR42_RS21230 0.51 +2.0 88,751 + RR42_RS21230 0.71 -2.8 88,751 + RR42_RS21230 0.71 -1.9 88,751 + RR42_RS21230 0.71 -1.3 88,751 + RR42_RS21230 0.71 +0.2 88,751 + RR42_RS21230 0.71 -0.6 88,751 + RR42_RS21230 0.71 -1.5 88,752 - RR42_RS21230 0.71 -2.5 88,752 - RR42_RS21230 0.71 -0.4 88,752 - RR42_RS21230 0.71 +0.2 88,752 - RR42_RS21230 0.71 +0.0 88,753 + RR42_RS21230 0.71 -1.1 88,754 - RR42_RS21230 0.71 +1.1 88,780 + RR42_RS21230 0.74 +0.1 88,780 + RR42_RS21230 0.74 +0.5 88,781 - RR42_RS21230 0.74 +0.5 88,794 - RR42_RS21230 0.75 -1.1 89,019 + +2.3 89,019 + +0.3 89,068 + +0.9 89,068 + -0.6 89,068 + +0.6 89,069 - -0.1 89,069 - +3.0 89,111 + -1.7 89,112 - -2.9 89,584 + RR42_RS21235 0.23 -0.5 89,584 + RR42_RS21235 0.23 -1.0 89,585 - RR42_RS21235 0.23 +0.2 89,585 - RR42_RS21235 0.23 +0.1 90,249 + RR42_RS21235 0.56 +0.1 90,250 - RR42_RS21235 0.56 +0.9 90,250 - RR42_RS21235 0.56 -2.2 90,288 - RR42_RS21235 0.58 +0.1 90,376 - RR42_RS21235 0.62 +0.1 90,542 + RR42_RS21235 0.70 +0.9 90,542 + RR42_RS21235 0.70 -0.9 90,542 + RR42_RS21235 0.70 -0.3 90,542 + RR42_RS21235 0.70 -2.0 90,542 + RR42_RS21235 0.70 +0.8 90,543 - RR42_RS21235 0.70 +1.2 90,543 - RR42_RS21235 0.70 -0.5 90,543 - RR42_RS21235 0.70 -3.0 90,909 - RR42_RS21235 0.88 +0.2 91,042 + -2.6 91,042 + +0.7 91,042 + +0.5 91,043 - +0.3 91,046 + -3.5 91,046 + +0.1 91,046 + -0.3 91,047 - +0.5 91,336 + -0.1 91,383 + -0.0 91,635 + +0.4 91,636 - +0.7 91,636 - -0.2 91,636 - -0.3 91,720 + -1.2 91,720 + +0.5
Or see this region's nucleotide sequence