Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21220 and RR42_RS21225 are separated by 19 nucleotides RR42_RS21225 and RR42_RS21230 are separated by 513 nucleotides RR42_RS21230 and RR42_RS21235 are separated by 70 nucleotides
RR42_RS21220: RR42_RS21220 - iron transporter FeoB, at 85,337 to 87,208
_RS21220
RR42_RS21225: RR42_RS21225 - iron transporter FeoA, at 87,228 to 87,533
_RS21225
RR42_RS21230: RR42_RS21230 - ABC transporter substrate-binding protein, at 88,047 to 89,039
_RS21230
RR42_RS21235: RR42_RS21235 - thymidine phosphorylase, at 89,110 to 91,149
_RS21235
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 87.079 kb on - strand at 87.209 kb on - strand at 87.209 kb on - strand at 87.209 kb on - strand at 87.597 kb on - strand at 87.619 kb on - strand at 87.989 kb on - strand at 88.048 kb on + strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.282 kb on + strand, within RR42_RS21230 at 88.283 kb on - strand, within RR42_RS21230 at 88.384 kb on + strand, within RR42_RS21230 at 88.384 kb on + strand, within RR42_RS21230 at 88.384 kb on + strand, within RR42_RS21230 at 88.385 kb on - strand, within RR42_RS21230 at 88.468 kb on + strand, within RR42_RS21230 at 88.468 kb on + strand, within RR42_RS21230 at 88.555 kb on + strand, within RR42_RS21230 at 88.556 kb on - strand, within RR42_RS21230 at 88.556 kb on - strand, within RR42_RS21230 at 88.556 kb on - strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.751 kb on + strand, within RR42_RS21230 at 88.752 kb on - strand, within RR42_RS21230 at 88.752 kb on - strand, within RR42_RS21230 at 88.752 kb on - strand, within RR42_RS21230 at 88.752 kb on - strand, within RR42_RS21230 at 88.753 kb on + strand, within RR42_RS21230 at 88.754 kb on - strand, within RR42_RS21230 at 88.780 kb on + strand, within RR42_RS21230 at 88.780 kb on + strand, within RR42_RS21230 at 88.781 kb on - strand, within RR42_RS21230 at 88.794 kb on - strand, within RR42_RS21230 at 89.019 kb on + strand at 89.019 kb on + strand at 89.068 kb on + strand at 89.068 kb on + strand at 89.068 kb on + strand at 89.069 kb on - strand at 89.069 kb on - strand at 89.111 kb on + strand at 89.112 kb on - strand at 89.584 kb on + strand, within RR42_RS21235 at 89.584 kb on + strand, within RR42_RS21235 at 89.585 kb on - strand, within RR42_RS21235 at 89.585 kb on - strand, within RR42_RS21235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 87,079 - +0.1 87,209 - +3.3 87,209 - +2.8 87,209 - +2.7 87,597 - +3.4 87,619 - +0.2 87,989 - +1.7 88,048 + +0.3 88,049 - -1.3 88,049 - -1.0 88,282 + RR42_RS21230 0.24 +1.1 88,283 - RR42_RS21230 0.24 -2.4 88,384 + RR42_RS21230 0.34 +0.5 88,384 + RR42_RS21230 0.34 -1.2 88,384 + RR42_RS21230 0.34 +0.5 88,385 - RR42_RS21230 0.34 +0.1 88,468 + RR42_RS21230 0.42 +1.0 88,468 + RR42_RS21230 0.42 +0.4 88,555 + RR42_RS21230 0.51 +0.5 88,556 - RR42_RS21230 0.51 +0.4 88,556 - RR42_RS21230 0.51 -0.2 88,556 - RR42_RS21230 0.51 +2.0 88,751 + RR42_RS21230 0.71 -2.8 88,751 + RR42_RS21230 0.71 -1.9 88,751 + RR42_RS21230 0.71 -1.3 88,751 + RR42_RS21230 0.71 +0.2 88,751 + RR42_RS21230 0.71 -0.6 88,751 + RR42_RS21230 0.71 -1.5 88,752 - RR42_RS21230 0.71 -2.5 88,752 - RR42_RS21230 0.71 -0.4 88,752 - RR42_RS21230 0.71 +0.2 88,752 - RR42_RS21230 0.71 +0.0 88,753 + RR42_RS21230 0.71 -1.1 88,754 - RR42_RS21230 0.71 +1.1 88,780 + RR42_RS21230 0.74 +0.1 88,780 + RR42_RS21230 0.74 +0.5 88,781 - RR42_RS21230 0.74 +0.5 88,794 - RR42_RS21230 0.75 -1.1 89,019 + +2.3 89,019 + +0.3 89,068 + +0.9 89,068 + -0.6 89,068 + +0.6 89,069 - -0.1 89,069 - +3.0 89,111 + -1.7 89,112 - -2.9 89,584 + RR42_RS21235 0.23 -0.5 89,584 + RR42_RS21235 0.23 -1.0 89,585 - RR42_RS21235 0.23 +0.2 89,585 - RR42_RS21235 0.23 +0.1
Or see this region's nucleotide sequence