Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20965 and RR42_RS20970 are separated by 63 nucleotides RR42_RS20970 and RR42_RS20975 overlap by 4 nucleotides
RR42_RS20965: RR42_RS20965 - transferase, at 30,936 to 32,123
_RS20965
RR42_RS20970: RR42_RS20970 - hypothetical protein, at 32,187 to 33,467
_RS20970
RR42_RS20975: RR42_RS20975 - hypothetical protein, at 33,464 to 34,282
_RS20975
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 31.438 kb on - strand, within RR42_RS20965 at 31.881 kb on - strand, within RR42_RS20965 at 31.881 kb on - strand, within RR42_RS20965 at 32.122 kb on - strand at 32.212 kb on - strand at 32.292 kb on + strand at 32.293 kb on - strand at 32.556 kb on + strand, within RR42_RS20970 at 32.676 kb on + strand, within RR42_RS20970 at 32.776 kb on + strand, within RR42_RS20970 at 32.826 kb on + strand, within RR42_RS20970 at 32.826 kb on + strand, within RR42_RS20970 at 32.827 kb on - strand, within RR42_RS20970 at 32.827 kb on - strand, within RR42_RS20970 at 32.827 kb on - strand, within RR42_RS20970 at 32.871 kb on + strand, within RR42_RS20970 at 32.872 kb on - strand, within RR42_RS20970 at 33.000 kb on + strand, within RR42_RS20970 at 33.122 kb on - strand, within RR42_RS20970 at 33.122 kb on - strand, within RR42_RS20970 at 33.147 kb on + strand, within RR42_RS20970 at 33.147 kb on + strand, within RR42_RS20970 at 33.147 kb on + strand, within RR42_RS20970 at 33.148 kb on - strand, within RR42_RS20970 at 33.628 kb on + strand, within RR42_RS20975 at 33.629 kb on - strand, within RR42_RS20975 at 33.687 kb on + strand, within RR42_RS20975 at 33.688 kb on - strand, within RR42_RS20975 at 34.151 kb on + strand, within RR42_RS20975 at 34.151 kb on + strand, within RR42_RS20975 at 34.166 kb on + strand, within RR42_RS20975 at 34.166 kb on + strand, within RR42_RS20975 at 34.167 kb on - strand, within RR42_RS20975 at 34.299 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 31,438 - RR42_RS20965 0.42 -1.2 31,881 - RR42_RS20965 0.80 -0.6 31,881 - RR42_RS20965 0.80 -1.3 32,122 - +1.2 32,212 - -1.5 32,292 + +0.7 32,293 - +0.2 32,556 + RR42_RS20970 0.29 +0.7 32,676 + RR42_RS20970 0.38 -0.7 32,776 + RR42_RS20970 0.46 -0.7 32,826 + RR42_RS20970 0.50 +0.8 32,826 + RR42_RS20970 0.50 +0.3 32,827 - RR42_RS20970 0.50 +0.2 32,827 - RR42_RS20970 0.50 +1.9 32,827 - RR42_RS20970 0.50 -2.0 32,871 + RR42_RS20970 0.53 -0.3 32,872 - RR42_RS20970 0.53 -0.7 33,000 + RR42_RS20970 0.63 +0.5 33,122 - RR42_RS20970 0.73 +0.3 33,122 - RR42_RS20970 0.73 +0.4 33,147 + RR42_RS20970 0.75 +0.6 33,147 + RR42_RS20970 0.75 -1.1 33,147 + RR42_RS20970 0.75 +0.6 33,148 - RR42_RS20970 0.75 -0.6 33,628 + RR42_RS20975 0.20 -0.5 33,629 - RR42_RS20975 0.20 -0.0 33,687 + RR42_RS20975 0.27 -0.3 33,688 - RR42_RS20975 0.27 +1.1 34,151 + RR42_RS20975 0.84 +1.1 34,151 + RR42_RS20975 0.84 -0.6 34,166 + RR42_RS20975 0.86 -1.7 34,166 + RR42_RS20975 0.86 -0.3 34,167 - RR42_RS20975 0.86 +0.2 34,299 - +1.3
Or see this region's nucleotide sequence