Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20870 and RR42_RS20875 are separated by 499 nucleotides RR42_RS20875 and RR42_RS20880 are separated by 337 nucleotides RR42_RS20880 and RR42_RS20885 are separated by 99 nucleotides
RR42_RS20870: RR42_RS20870 - integrase, at 5,147 to 6,730
_RS20870
RR42_RS20875: RR42_RS20875 - LuxR family transcriptional regulator, at 7,230 to 8,090
_RS20875
RR42_RS20880: RR42_RS20880 - hypothetical protein, at 8,428 to 8,877
_RS20880
RR42_RS20885: RR42_RS20885 - hypothetical protein, at 8,977 to 9,243
_RS20885
Position (kb) 7
8
9 Strain fitness (log2 ratio) -2
-1
0
1
2 at 6.787 kb on + strand at 6.803 kb on + strand at 6.939 kb on + strand at 6.939 kb on + strand at 6.940 kb on - strand at 6.972 kb on + strand at 7.102 kb on + strand at 7.102 kb on + strand at 7.136 kb on - strand at 7.157 kb on - strand at 7.209 kb on - strand at 7.342 kb on + strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.348 kb on - strand, within RR42_RS20875 at 7.387 kb on - strand, within RR42_RS20875 at 7.691 kb on + strand, within RR42_RS20875 at 7.691 kb on + strand, within RR42_RS20875 at 7.692 kb on - strand, within RR42_RS20875 at 7.692 kb on - strand, within RR42_RS20875 at 7.811 kb on + strand, within RR42_RS20875 at 7.812 kb on - strand, within RR42_RS20875 at 7.812 kb on - strand, within RR42_RS20875 at 7.812 kb on - strand, within RR42_RS20875 at 7.814 kb on + strand, within RR42_RS20875 at 7.814 kb on + strand, within RR42_RS20875 at 7.814 kb on + strand, within RR42_RS20875 at 7.817 kb on + strand, within RR42_RS20875 at 7.831 kb on - strand, within RR42_RS20875 at 7.972 kb on - strand, within RR42_RS20875 at 8.096 kb on - strand at 8.211 kb on + strand at 8.211 kb on + strand at 8.310 kb on - strand at 8.325 kb on - strand at 8.325 kb on - strand at 8.383 kb on - strand at 8.561 kb on + strand, within RR42_RS20880 at 8.562 kb on - strand, within RR42_RS20880 at 8.707 kb on + strand, within RR42_RS20880 at 8.707 kb on + strand, within RR42_RS20880 at 8.864 kb on - strand at 8.900 kb on + strand at 8.901 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 6,787 + +0.8 6,803 + +1.1 6,939 + -0.2 6,939 + -0.9 6,940 - +0.3 6,972 + -0.6 7,102 + +1.1 7,102 + -1.9 7,136 - -2.4 7,157 - +0.1 7,209 - +0.7 7,342 + RR42_RS20875 0.13 +0.7 7,343 - RR42_RS20875 0.13 -1.1 7,343 - RR42_RS20875 0.13 +1.2 7,343 - RR42_RS20875 0.13 -1.7 7,343 - RR42_RS20875 0.13 +1.0 7,343 - RR42_RS20875 0.13 -0.7 7,343 - RR42_RS20875 0.13 -2.0 7,348 - RR42_RS20875 0.14 -2.4 7,387 - RR42_RS20875 0.18 -0.3 7,691 + RR42_RS20875 0.54 -1.5 7,691 + RR42_RS20875 0.54 -1.9 7,692 - RR42_RS20875 0.54 -1.5 7,692 - RR42_RS20875 0.54 +0.3 7,811 + RR42_RS20875 0.67 +0.4 7,812 - RR42_RS20875 0.68 +0.7 7,812 - RR42_RS20875 0.68 +0.3 7,812 - RR42_RS20875 0.68 -1.3 7,814 + RR42_RS20875 0.68 -2.5 7,814 + RR42_RS20875 0.68 +2.6 7,814 + RR42_RS20875 0.68 -0.7 7,817 + RR42_RS20875 0.68 -1.3 7,831 - RR42_RS20875 0.70 -0.6 7,972 - RR42_RS20875 0.86 +1.9 8,096 - +0.7 8,211 + +0.6 8,211 + -0.7 8,310 - -0.6 8,325 - +1.0 8,325 - +1.7 8,383 - -0.6 8,561 + RR42_RS20880 0.30 -0.6 8,562 - RR42_RS20880 0.30 +0.2 8,707 + RR42_RS20880 0.62 -0.2 8,707 + RR42_RS20880 0.62 -1.4 8,864 - +0.6 8,900 + -0.7 8,901 - -0.5
Or see this region's nucleotide sequence