Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS20480

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS20475 and RR42_RS20480 overlap by 4 nucleotidesRR42_RS20480 and RR42_RS20485 are separated by 98 nucleotidesRR42_RS20485 and RR42_RS20490 are separated by 269 nucleotides RR42_RS20475: RR42_RS20475 - peptidoglycan-binding protein LysM, at 4,435,344 to 4,436,516 _RS20475 RR42_RS20480: RR42_RS20480 - DNA processing protein DprA, at 4,436,513 to 4,437,658 _RS20480 RR42_RS20485: RR42_RS20485 - thiol-disulfide isomerase, at 4,437,757 to 4,438,161 _RS20485 RR42_RS20490: RR42_RS20490 - DNA topoisomerase III, at 4,438,431 to 4,441,145 _RS20490 Position (kb) 4436 4437 4438Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 4435.560 kb on + strand, within RR42_RS20475at 4435.560 kb on + strand, within RR42_RS20475at 4435.560 kb on + strand, within RR42_RS20475at 4435.560 kb on + strand, within RR42_RS20475at 4435.560 kb on + strand, within RR42_RS20475at 4435.560 kb on + strand, within RR42_RS20475at 4435.560 kb on + strand, within RR42_RS20475at 4435.560 kb on + strand, within RR42_RS20475at 4435.561 kb on - strand, within RR42_RS20475at 4435.561 kb on - strand, within RR42_RS20475at 4435.561 kb on - strand, within RR42_RS20475at 4435.561 kb on - strand, within RR42_RS20475at 4435.561 kb on - strand, within RR42_RS20475at 4435.561 kb on - strand, within RR42_RS20475at 4435.678 kb on + strand, within RR42_RS20475at 4435.678 kb on + strand, within RR42_RS20475at 4435.678 kb on + strand, within RR42_RS20475at 4435.678 kb on + strand, within RR42_RS20475at 4435.678 kb on + strand, within RR42_RS20475at 4435.678 kb on + strand, within RR42_RS20475at 4435.678 kb on + strand, within RR42_RS20475at 4435.679 kb on - strand, within RR42_RS20475at 4435.679 kb on - strand, within RR42_RS20475at 4435.679 kb on - strand, within RR42_RS20475at 4435.679 kb on - strand, within RR42_RS20475at 4435.679 kb on - strand, within RR42_RS20475at 4435.679 kb on - strand, within RR42_RS20475at 4435.679 kb on - strand, within RR42_RS20475at 4435.679 kb on - strand, within RR42_RS20475at 4435.679 kb on - strand, within RR42_RS20475at 4435.680 kb on + strand, within RR42_RS20475at 4435.680 kb on + strand, within RR42_RS20475at 4435.681 kb on - strand, within RR42_RS20475at 4435.681 kb on - strand, within RR42_RS20475at 4435.681 kb on - strand, within RR42_RS20475at 4435.681 kb on - strand, within RR42_RS20475at 4435.681 kb on - strand, within RR42_RS20475at 4435.681 kb on - strand, within RR42_RS20475at 4435.681 kb on - strand, within RR42_RS20475at 4435.698 kb on + strand, within RR42_RS20475at 4435.699 kb on - strand, within RR42_RS20475at 4435.921 kb on - strand, within RR42_RS20475at 4435.945 kb on - strand, within RR42_RS20475at 4436.050 kb on - strand, within RR42_RS20475at 4436.110 kb on + strand, within RR42_RS20475at 4436.111 kb on - strand, within RR42_RS20475at 4436.111 kb on - strand, within RR42_RS20475at 4436.111 kb on - strand, within RR42_RS20475at 4436.111 kb on - strand, within RR42_RS20475at 4436.111 kb on - strand, within RR42_RS20475at 4436.111 kb on - strand, within RR42_RS20475at 4436.114 kb on + strand, within RR42_RS20475at 4436.114 kb on + strand, within RR42_RS20475at 4436.115 kb on - strand, within RR42_RS20475at 4436.115 kb on - strand, within RR42_RS20475at 4436.115 kb on - strand, within RR42_RS20475at 4436.196 kb on + strand, within RR42_RS20475at 4436.196 kb on + strand, within RR42_RS20475at 4436.197 kb on - strand, within RR42_RS20475at 4436.197 kb on - strand, within RR42_RS20475at 4436.197 kb on - strand, within RR42_RS20475at 4436.197 kb on - strand, within RR42_RS20475at 4436.247 kb on + strand, within RR42_RS20475at 4436.248 kb on - strand, within RR42_RS20475at 4436.409 kb on + strandat 4436.452 kb on - strandat 4436.835 kb on - strand, within RR42_RS20480at 4436.879 kb on + strand, within RR42_RS20480at 4436.879 kb on + strand, within RR42_RS20480at 4436.879 kb on + strand, within RR42_RS20480at 4436.880 kb on - strand, within RR42_RS20480at 4436.880 kb on - strand, within RR42_RS20480at 4436.880 kb on - strand, within RR42_RS20480at 4437.329 kb on + strand, within RR42_RS20480at 4437.329 kb on + strand, within RR42_RS20480at 4437.329 kb on + strand, within RR42_RS20480at 4437.329 kb on + strand, within RR42_RS20480at 4437.330 kb on - strand, within RR42_RS20480at 4437.330 kb on - strand, within RR42_RS20480at 4437.330 kb on - strand, within RR42_RS20480at 4437.330 kb on - strand, within RR42_RS20480at 4437.330 kb on - strand, within RR42_RS20480at 4437.330 kb on - strand, within RR42_RS20480at 4437.635 kb on - strandat 4437.641 kb on + strandat 4437.641 kb on + strandat 4437.651 kb on + strandat 4437.652 kb on - strandat 4437.652 kb on - strandat 4437.652 kb on - strandat 4437.652 kb on - strandat 4437.652 kb on - strandat 4437.652 kb on - strandat 4437.652 kb on - strandat 4437.652 kb on - strandat 4437.656 kb on + strandat 4437.656 kb on + strandat 4437.656 kb on + strandat 4437.657 kb on - strandat 4437.657 kb on - strandat 4437.687 kb on + strandat 4437.687 kb on + strandat 4437.687 kb on + strandat 4437.709 kb on - strandat 4437.766 kb on + strandat 4437.766 kb on + strandat 4437.766 kb on + strandat 4437.766 kb on + strandat 4437.766 kb on + strandat 4437.833 kb on + strand, within RR42_RS20485at 4437.834 kb on + strand, within RR42_RS20485at 4437.875 kb on - strand, within RR42_RS20485at 4437.875 kb on - strand, within RR42_RS20485at 4437.875 kb on - strand, within RR42_RS20485at 4437.973 kb on - strand, within RR42_RS20485at 4438.030 kb on + strand, within RR42_RS20485at 4438.050 kb on + strand, within RR42_RS20485at 4438.050 kb on + strand, within RR42_RS20485at 4438.051 kb on - strand, within RR42_RS20485at 4438.051 kb on - strand, within RR42_RS20485at 4438.324 kb on + strandat 4438.324 kb on + strandat 4438.327 kb on + strandat 4438.359 kb on + strandat 4438.359 kb on + strandat 4438.359 kb on + strandat 4438.359 kb on + strandat 4438.359 kb on + strandat 4438.363 kb on + strandat 4438.363 kb on + strandat 4438.363 kb on + strandat 4438.363 kb on + strandat 4438.363 kb on + strandat 4438.363 kb on + strandat 4438.363 kb on + strandat 4438.363 kb on + strandat 4438.364 kb on - strandat 4438.365 kb on + strandat 4438.365 kb on + strandat 4438.365 kb on + strandat 4438.365 kb on + strandat 4438.365 kb on + strandat 4438.365 kb on + strandat 4438.365 kb on + strandat 4438.379 kb on + strandat 4438.379 kb on + strandat 4438.379 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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4,435,560 + RR42_RS20475 0.18 +0.4
4,435,560 + RR42_RS20475 0.18 +2.0
4,435,560 + RR42_RS20475 0.18 -0.0
4,435,560 + RR42_RS20475 0.18 +0.1
4,435,560 + RR42_RS20475 0.18 +2.3
4,435,560 + RR42_RS20475 0.18 -2.4
4,435,560 + RR42_RS20475 0.18 +0.5
4,435,560 + RR42_RS20475 0.18 +0.1
4,435,561 - RR42_RS20475 0.18 -0.1
4,435,561 - RR42_RS20475 0.18 -0.4
4,435,561 - RR42_RS20475 0.18 -0.1
4,435,561 - RR42_RS20475 0.18 -2.2
4,435,561 - RR42_RS20475 0.18 -1.2
4,435,561 - RR42_RS20475 0.18 -2.6
4,435,678 + RR42_RS20475 0.28 -0.0
4,435,678 + RR42_RS20475 0.28 -0.2
4,435,678 + RR42_RS20475 0.28 -1.6
4,435,678 + RR42_RS20475 0.28 -2.5
4,435,678 + RR42_RS20475 0.28 -1.1
4,435,678 + RR42_RS20475 0.28 -2.0
4,435,678 + RR42_RS20475 0.28 -0.2
4,435,679 - RR42_RS20475 0.29 +1.7
4,435,679 - RR42_RS20475 0.29 +0.6
4,435,679 - RR42_RS20475 0.29 +0.4
4,435,679 - RR42_RS20475 0.29 +1.4
4,435,679 - RR42_RS20475 0.29 -0.1
4,435,679 - RR42_RS20475 0.29 +1.9
4,435,679 - RR42_RS20475 0.29 +1.4
4,435,679 - RR42_RS20475 0.29 -0.1
4,435,679 - RR42_RS20475 0.29 -0.6
4,435,680 + RR42_RS20475 0.29 -0.2
4,435,680 + RR42_RS20475 0.29 -0.1
4,435,681 - RR42_RS20475 0.29 +0.4
4,435,681 - RR42_RS20475 0.29 +0.7
4,435,681 - RR42_RS20475 0.29 -0.1
4,435,681 - RR42_RS20475 0.29 +0.0
4,435,681 - RR42_RS20475 0.29 -1.1
4,435,681 - RR42_RS20475 0.29 +0.4
4,435,681 - RR42_RS20475 0.29 +0.3
4,435,698 + RR42_RS20475 0.30 +2.3
4,435,699 - RR42_RS20475 0.30 +0.1
4,435,921 - RR42_RS20475 0.49 -1.2
4,435,945 - RR42_RS20475 0.51 -0.1
4,436,050 - RR42_RS20475 0.60 -0.6
4,436,110 + RR42_RS20475 0.65 +0.8
4,436,111 - RR42_RS20475 0.65 -0.2
4,436,111 - RR42_RS20475 0.65 +0.1
4,436,111 - RR42_RS20475 0.65 +0.7
4,436,111 - RR42_RS20475 0.65 +0.4
4,436,111 - RR42_RS20475 0.65 -0.5
4,436,111 - RR42_RS20475 0.65 -1.9
4,436,114 + RR42_RS20475 0.66 -1.1
4,436,114 + RR42_RS20475 0.66 +0.1
4,436,115 - RR42_RS20475 0.66 +0.2
4,436,115 - RR42_RS20475 0.66 -0.4
4,436,115 - RR42_RS20475 0.66 -0.9
4,436,196 + RR42_RS20475 0.73 -2.2
4,436,196 + RR42_RS20475 0.73 -0.1
4,436,197 - RR42_RS20475 0.73 +0.3
4,436,197 - RR42_RS20475 0.73 +0.1
4,436,197 - RR42_RS20475 0.73 +0.6
4,436,197 - RR42_RS20475 0.73 +0.9
4,436,247 + RR42_RS20475 0.77 +0.1
4,436,248 - RR42_RS20475 0.77 +0.3
4,436,409 + +2.1
4,436,452 - +0.6
4,436,835 - RR42_RS20480 0.28 -0.4
4,436,879 + RR42_RS20480 0.32 +1.1
4,436,879 + RR42_RS20480 0.32 +0.8
4,436,879 + RR42_RS20480 0.32 -1.2
4,436,880 - RR42_RS20480 0.32 +0.2
4,436,880 - RR42_RS20480 0.32 -2.9
4,436,880 - RR42_RS20480 0.32 +0.4
4,437,329 + RR42_RS20480 0.71 -0.8
4,437,329 + RR42_RS20480 0.71 -1.2
4,437,329 + RR42_RS20480 0.71 -0.8
4,437,329 + RR42_RS20480 0.71 -1.2
4,437,330 - RR42_RS20480 0.71 +1.0
4,437,330 - RR42_RS20480 0.71 +1.8
4,437,330 - RR42_RS20480 0.71 +0.1
4,437,330 - RR42_RS20480 0.71 -2.0
4,437,330 - RR42_RS20480 0.71 -1.2
4,437,330 - RR42_RS20480 0.71 -1.7
4,437,635 - -2.0
4,437,641 + +1.3
4,437,641 + +0.5
4,437,651 + -0.0
4,437,652 - -1.2
4,437,652 - -0.1
4,437,652 - +1.8
4,437,652 - -0.2
4,437,652 - -0.7
4,437,652 - +0.8
4,437,652 - -0.4
4,437,652 - +0.4
4,437,656 + +0.4
4,437,656 + -1.4
4,437,656 + -0.5
4,437,657 - +0.6
4,437,657 - +1.4
4,437,687 + +1.0
4,437,687 + +0.4
4,437,687 + +0.1
4,437,709 - +1.6
4,437,766 + -0.2
4,437,766 + +0.3
4,437,766 + +0.2
4,437,766 + -0.1
4,437,766 + -0.4
4,437,833 + RR42_RS20485 0.19 -0.5
4,437,834 + RR42_RS20485 0.19 -0.9
4,437,875 - RR42_RS20485 0.29 +1.8
4,437,875 - RR42_RS20485 0.29 +0.4
4,437,875 - RR42_RS20485 0.29 +0.2
4,437,973 - RR42_RS20485 0.53 +1.5
4,438,030 + RR42_RS20485 0.67 -0.7
4,438,050 + RR42_RS20485 0.72 -1.9
4,438,050 + RR42_RS20485 0.72 -0.6
4,438,051 - RR42_RS20485 0.73 -1.2
4,438,051 - RR42_RS20485 0.73 -2.5
4,438,324 + +0.1
4,438,324 + -0.7
4,438,327 + +1.3
4,438,359 + +4.0
4,438,359 + +1.2
4,438,359 + +0.7
4,438,359 + +0.4
4,438,359 + -1.8
4,438,363 + +0.1
4,438,363 + -1.8
4,438,363 + -1.2
4,438,363 + -0.4
4,438,363 + +0.6
4,438,363 + -1.5
4,438,363 + -0.2
4,438,363 + -0.6
4,438,364 - +0.3
4,438,365 + -1.8
4,438,365 + +0.6
4,438,365 + -0.2
4,438,365 + -0.2
4,438,365 + -1.2
4,438,365 + -0.7
4,438,365 + +1.1
4,438,379 + -2.3
4,438,379 + -2.4
4,438,379 + -0.2

Or see this region's nucleotide sequence