Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20455 and RR42_RS20460 are separated by 185 nucleotides RR42_RS20460 and RR42_RS20465 are separated by 93 nucleotides
RR42_RS20455: RR42_RS20455 - protease HtpX, at 4,431,759 to 4,432,619
_RS20455
RR42_RS20460: RR42_RS20460 - lysine transporter LysE, at 4,432,805 to 4,433,446
_RS20460
RR42_RS20465: RR42_RS20465 - methionyl-tRNA formyltransferase, at 4,433,540 to 4,434,553
_RS20465
Position (kb) 4432
4433
4434 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4431.935 kb on - strand, within RR42_RS20455 at 4432.354 kb on + strand, within RR42_RS20455 at 4432.408 kb on + strand, within RR42_RS20455 at 4432.450 kb on + strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.483 kb on + strand, within RR42_RS20455 at 4432.483 kb on + strand, within RR42_RS20455 at 4432.483 kb on + strand, within RR42_RS20455 at 4432.484 kb on - strand, within RR42_RS20455 at 4432.484 kb on - strand, within RR42_RS20455 at 4432.484 kb on - strand, within RR42_RS20455 at 4432.678 kb on + strand at 4432.704 kb on + strand at 4432.704 kb on + strand at 4432.704 kb on + strand at 4432.704 kb on + strand at 4432.705 kb on - strand at 4432.705 kb on - strand at 4432.705 kb on - strand at 4432.705 kb on - strand at 4432.708 kb on + strand at 4432.708 kb on + strand at 4432.709 kb on - strand at 4432.711 kb on + strand at 4432.806 kb on + strand at 4432.807 kb on - strand at 4433.199 kb on + strand, within RR42_RS20460 at 4433.227 kb on + strand, within RR42_RS20460 at 4433.228 kb on - strand, within RR42_RS20460 at 4433.365 kb on + strand, within RR42_RS20460 at 4433.366 kb on - strand, within RR42_RS20460 at 4433.366 kb on - strand, within RR42_RS20460 at 4433.367 kb on + strand, within RR42_RS20460 at 4433.367 kb on + strand, within RR42_RS20460 at 4433.367 kb on + strand, within RR42_RS20460 at 4433.367 kb on + strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.434 kb on + strand at 4433.434 kb on + strand at 4433.435 kb on - strand at 4433.435 kb on - strand at 4433.469 kb on + strand at 4433.470 kb on - strand at 4433.541 kb on + strand at 4433.541 kb on + strand at 4433.541 kb on + strand at 4433.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 4,431,935 - RR42_RS20455 0.20 -0.2 4,432,354 + RR42_RS20455 0.69 +0.6 4,432,408 + RR42_RS20455 0.75 +0.2 4,432,450 + RR42_RS20455 0.80 +1.4 4,432,451 - RR42_RS20455 0.80 -0.3 4,432,451 - RR42_RS20455 0.80 -0.9 4,432,451 - RR42_RS20455 0.80 -0.3 4,432,451 - RR42_RS20455 0.80 +0.3 4,432,451 - RR42_RS20455 0.80 +0.3 4,432,483 + RR42_RS20455 0.84 -1.1 4,432,483 + RR42_RS20455 0.84 -0.7 4,432,483 + RR42_RS20455 0.84 +0.8 4,432,484 - RR42_RS20455 0.84 +0.1 4,432,484 - RR42_RS20455 0.84 -1.2 4,432,484 - RR42_RS20455 0.84 +0.1 4,432,678 + -0.8 4,432,704 + +0.4 4,432,704 + +0.4 4,432,704 + -0.1 4,432,704 + +0.8 4,432,705 - +1.2 4,432,705 - +1.0 4,432,705 - +0.7 4,432,705 - -3.0 4,432,708 + -1.2 4,432,708 + +0.3 4,432,709 - +0.6 4,432,711 + -0.4 4,432,806 + -1.3 4,432,807 - +0.3 4,433,199 + RR42_RS20460 0.61 -0.2 4,433,227 + RR42_RS20460 0.66 -1.3 4,433,228 - RR42_RS20460 0.66 -2.5 4,433,365 + RR42_RS20460 0.87 -0.6 4,433,366 - RR42_RS20460 0.87 +1.6 4,433,366 - RR42_RS20460 0.87 -1.5 4,433,367 + RR42_RS20460 0.88 -1.0 4,433,367 + RR42_RS20460 0.88 +0.7 4,433,367 + RR42_RS20460 0.88 -0.9 4,433,367 + RR42_RS20460 0.88 -0.7 4,433,368 - RR42_RS20460 0.88 -0.3 4,433,368 - RR42_RS20460 0.88 +0.4 4,433,368 - RR42_RS20460 0.88 +0.8 4,433,368 - RR42_RS20460 0.88 +0.4 4,433,368 - RR42_RS20460 0.88 -0.5 4,433,434 + -1.5 4,433,434 + +0.3 4,433,435 - +0.1 4,433,435 - +0.0 4,433,469 + +1.4 4,433,470 - -0.4 4,433,541 + -0.7 4,433,541 + +0.1 4,433,541 + -0.6 4,433,542 - -0.1
Or see this region's nucleotide sequence