Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20345 and RR42_RS20350 overlap by 4 nucleotides RR42_RS20350 and RR42_RS20355 overlap by 4 nucleotides RR42_RS20355 and RR42_RS20360 are separated by 88 nucleotides RR42_RS20360 and RR42_RS20365 are separated by 306 nucleotides
RR42_RS20345: RR42_RS20345 - penicillin-binding protein, at 4,400,921 to 4,403,125
_RS20345
RR42_RS20350: RR42_RS20350 - hypothetical protein, at 4,403,122 to 4,403,823
_RS20350
RR42_RS20355: RR42_RS20355 - alpha-2-macroglobulin, at 4,403,820 to 4,409,840
_RS20355
RR42_RS20360: RR42_RS20360 - AsnC family transcriptional regulator, at 4,409,929 to 4,410,408
_RS20360
RR42_RS20365: RR42_RS20365 - phenylalanine-4-hydroxylase, at 4,410,715 to 4,411,647
_RS20365
Position (kb)
4403
4404
4405
4406
4407
4408
4409
4410 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4402.861 kb on - strand, within RR42_RS20345 at 4402.861 kb on - strand, within RR42_RS20345 at 4403.205 kb on + strand, within RR42_RS20350 at 4403.342 kb on + strand, within RR42_RS20350 at 4403.343 kb on - strand, within RR42_RS20350 at 4403.748 kb on - strand, within RR42_RS20350 at 4403.868 kb on + strand at 4403.868 kb on + strand at 4403.881 kb on + strand at 4403.881 kb on + strand at 4403.974 kb on + strand at 4403.974 kb on + strand at 4403.975 kb on - strand at 4403.980 kb on + strand at 4403.981 kb on - strand at 4403.981 kb on - strand at 4404.172 kb on + strand at 4404.172 kb on + strand at 4404.173 kb on - strand at 4404.909 kb on + strand, within RR42_RS20355 at 4405.078 kb on + strand, within RR42_RS20355 at 4405.157 kb on - strand, within RR42_RS20355 at 4405.172 kb on + strand, within RR42_RS20355 at 4405.173 kb on - strand, within RR42_RS20355 at 4405.250 kb on - strand, within RR42_RS20355 at 4405.368 kb on + strand, within RR42_RS20355 at 4405.710 kb on - strand, within RR42_RS20355 at 4405.710 kb on - strand, within RR42_RS20355 at 4405.938 kb on - strand, within RR42_RS20355 at 4406.002 kb on + strand, within RR42_RS20355 at 4406.053 kb on + strand, within RR42_RS20355 at 4406.053 kb on + strand, within RR42_RS20355 at 4406.054 kb on - strand, within RR42_RS20355 at 4406.218 kb on + strand, within RR42_RS20355 at 4406.258 kb on - strand, within RR42_RS20355 at 4406.342 kb on - strand, within RR42_RS20355 at 4406.413 kb on + strand, within RR42_RS20355 at 4406.414 kb on - strand, within RR42_RS20355 at 4406.461 kb on + strand, within RR42_RS20355 at 4406.461 kb on + strand, within RR42_RS20355 at 4406.461 kb on + strand, within RR42_RS20355 at 4406.461 kb on + strand, within RR42_RS20355 at 4406.461 kb on + strand, within RR42_RS20355 at 4406.461 kb on + strand, within RR42_RS20355 at 4406.461 kb on + strand, within RR42_RS20355 at 4406.461 kb on + strand, within RR42_RS20355 at 4406.462 kb on - strand, within RR42_RS20355 at 4406.462 kb on - strand, within RR42_RS20355 at 4406.462 kb on - strand, within RR42_RS20355 at 4406.462 kb on - strand, within RR42_RS20355 at 4406.488 kb on - strand, within RR42_RS20355 at 4406.488 kb on - strand, within RR42_RS20355 at 4406.730 kb on + strand, within RR42_RS20355 at 4406.881 kb on + strand, within RR42_RS20355 at 4407.249 kb on - strand, within RR42_RS20355 at 4407.249 kb on - strand, within RR42_RS20355 at 4407.319 kb on + strand, within RR42_RS20355 at 4407.319 kb on + strand, within RR42_RS20355 at 4407.319 kb on + strand, within RR42_RS20355 at 4407.319 kb on + strand, within RR42_RS20355 at 4407.320 kb on - strand, within RR42_RS20355 at 4407.346 kb on + strand, within RR42_RS20355 at 4407.347 kb on - strand, within RR42_RS20355 at 4407.409 kb on + strand, within RR42_RS20355 at 4407.409 kb on + strand, within RR42_RS20355 at 4407.410 kb on - strand, within RR42_RS20355 at 4407.410 kb on - strand, within RR42_RS20355 at 4407.410 kb on - strand, within RR42_RS20355 at 4407.566 kb on + strand, within RR42_RS20355 at 4407.566 kb on + strand, within RR42_RS20355 at 4407.567 kb on - strand, within RR42_RS20355 at 4407.567 kb on - strand, within RR42_RS20355 at 4407.569 kb on - strand, within RR42_RS20355 at 4407.569 kb on - strand, within RR42_RS20355 at 4407.800 kb on + strand, within RR42_RS20355 at 4407.801 kb on - strand, within RR42_RS20355 at 4407.968 kb on - strand, within RR42_RS20355 at 4408.222 kb on + strand, within RR42_RS20355 at 4408.223 kb on - strand, within RR42_RS20355 at 4408.223 kb on - strand, within RR42_RS20355 at 4408.223 kb on - strand, within RR42_RS20355 at 4408.955 kb on + strand, within RR42_RS20355 at 4408.955 kb on + strand, within RR42_RS20355 at 4408.956 kb on - strand, within RR42_RS20355 at 4408.956 kb on - strand, within RR42_RS20355 at 4409.051 kb on + strand, within RR42_RS20355 at 4409.052 kb on - strand, within RR42_RS20355 at 4409.052 kb on - strand, within RR42_RS20355 at 4409.084 kb on - strand, within RR42_RS20355 at 4409.084 kb on - strand, within RR42_RS20355 at 4409.084 kb on - strand, within RR42_RS20355 at 4409.446 kb on + strand at 4409.447 kb on - strand at 4409.464 kb on + strand at 4409.465 kb on - strand at 4410.083 kb on + strand, within RR42_RS20360 at 4410.107 kb on + strand, within RR42_RS20360 at 4410.512 kb on + strand at 4410.513 kb on - strand at 4410.521 kb on + strand at 4410.592 kb on + strand at 4410.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 4,402,861 - RR42_RS20345 0.88 -2.4 4,402,861 - RR42_RS20345 0.88 -1.1 4,403,205 + RR42_RS20350 0.12 +0.7 4,403,342 + RR42_RS20350 0.31 -2.0 4,403,343 - RR42_RS20350 0.31 -1.2 4,403,748 - RR42_RS20350 0.89 +1.0 4,403,868 + +0.1 4,403,868 + -0.2 4,403,881 + -1.1 4,403,881 + +0.0 4,403,974 + -2.5 4,403,974 + +0.3 4,403,975 - +0.7 4,403,980 + +1.2 4,403,981 - -0.1 4,403,981 - +1.1 4,404,172 + +2.0 4,404,172 + -0.7 4,404,173 - +0.4 4,404,909 + RR42_RS20355 0.18 +1.8 4,405,078 + RR42_RS20355 0.21 -1.6 4,405,157 - RR42_RS20355 0.22 +0.2 4,405,172 + RR42_RS20355 0.22 +0.6 4,405,173 - RR42_RS20355 0.22 +2.4 4,405,250 - RR42_RS20355 0.24 +1.1 4,405,368 + RR42_RS20355 0.26 +0.7 4,405,710 - RR42_RS20355 0.31 -1.2 4,405,710 - RR42_RS20355 0.31 -0.3 4,405,938 - RR42_RS20355 0.35 -0.9 4,406,002 + RR42_RS20355 0.36 +0.3 4,406,053 + RR42_RS20355 0.37 +0.2 4,406,053 + RR42_RS20355 0.37 -0.6 4,406,054 - RR42_RS20355 0.37 +0.6 4,406,218 + RR42_RS20355 0.40 -1.4 4,406,258 - RR42_RS20355 0.40 -0.1 4,406,342 - RR42_RS20355 0.42 +0.1 4,406,413 + RR42_RS20355 0.43 -0.9 4,406,414 - RR42_RS20355 0.43 +0.6 4,406,461 + RR42_RS20355 0.44 -0.7 4,406,461 + RR42_RS20355 0.44 -2.3 4,406,461 + RR42_RS20355 0.44 -1.6 4,406,461 + RR42_RS20355 0.44 +0.8 4,406,461 + RR42_RS20355 0.44 -3.6 4,406,461 + RR42_RS20355 0.44 -1.9 4,406,461 + RR42_RS20355 0.44 +0.7 4,406,461 + RR42_RS20355 0.44 +1.6 4,406,462 - RR42_RS20355 0.44 +1.2 4,406,462 - RR42_RS20355 0.44 -1.4 4,406,462 - RR42_RS20355 0.44 +0.5 4,406,462 - RR42_RS20355 0.44 +0.2 4,406,488 - RR42_RS20355 0.44 -0.2 4,406,488 - RR42_RS20355 0.44 -0.3 4,406,730 + RR42_RS20355 0.48 -0.4 4,406,881 + RR42_RS20355 0.51 -2.5 4,407,249 - RR42_RS20355 0.57 +0.4 4,407,249 - RR42_RS20355 0.57 -1.8 4,407,319 + RR42_RS20355 0.58 +0.2 4,407,319 + RR42_RS20355 0.58 +1.6 4,407,319 + RR42_RS20355 0.58 +0.7 4,407,319 + RR42_RS20355 0.58 +0.0 4,407,320 - RR42_RS20355 0.58 -0.0 4,407,346 + RR42_RS20355 0.59 +0.5 4,407,347 - RR42_RS20355 0.59 +0.3 4,407,409 + RR42_RS20355 0.60 -0.6 4,407,409 + RR42_RS20355 0.60 -1.1 4,407,410 - RR42_RS20355 0.60 -1.0 4,407,410 - RR42_RS20355 0.60 -1.7 4,407,410 - RR42_RS20355 0.60 +1.4 4,407,566 + RR42_RS20355 0.62 +0.4 4,407,566 + RR42_RS20355 0.62 -0.2 4,407,567 - RR42_RS20355 0.62 +1.3 4,407,567 - RR42_RS20355 0.62 -0.2 4,407,569 - RR42_RS20355 0.62 +0.1 4,407,569 - RR42_RS20355 0.62 +0.6 4,407,800 + RR42_RS20355 0.66 +0.2 4,407,801 - RR42_RS20355 0.66 +1.0 4,407,968 - RR42_RS20355 0.69 +1.1 4,408,222 + RR42_RS20355 0.73 +0.8 4,408,223 - RR42_RS20355 0.73 +1.4 4,408,223 - RR42_RS20355 0.73 -2.3 4,408,223 - RR42_RS20355 0.73 +0.2 4,408,955 + RR42_RS20355 0.85 +1.2 4,408,955 + RR42_RS20355 0.85 -2.1 4,408,956 - RR42_RS20355 0.85 +1.4 4,408,956 - RR42_RS20355 0.85 +0.9 4,409,051 + RR42_RS20355 0.87 -1.2 4,409,052 - RR42_RS20355 0.87 +1.2 4,409,052 - RR42_RS20355 0.87 +2.5 4,409,084 - RR42_RS20355 0.87 -0.4 4,409,084 - RR42_RS20355 0.87 -1.6 4,409,084 - RR42_RS20355 0.87 +0.1 4,409,446 + -0.8 4,409,447 - -0.4 4,409,464 + +0.1 4,409,465 - -0.3 4,410,083 + RR42_RS20360 0.32 -0.3 4,410,107 + RR42_RS20360 0.37 -0.4 4,410,512 + +0.5 4,410,513 - -0.3 4,410,521 + +1.7 4,410,592 + -0.4 4,410,720 + +0.7
Or see this region's nucleotide sequence