Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20275 and RR42_RS20280 are separated by 95 nucleotides RR42_RS20280 and RR42_RS20285 are separated by 9 nucleotides RR42_RS20285 and RR42_RS20290 are separated by 381 nucleotides
RR42_RS20275: RR42_RS20275 - ABC transporter permease, at 4,385,888 to 4,387,096
_RS20275
RR42_RS20280: RR42_RS20280 - histidinol dehydrogenase, at 4,387,192 to 4,387,917
_RS20280
RR42_RS20285: RR42_RS20285 - amino acid ABC transporter ATP-binding protein, at 4,387,927 to 4,388,703
_RS20285
RR42_RS20290: RR42_RS20290 - choline dehydrogenase, at 4,389,085 to 4,390,785
_RS20290
Position (kb)
4387
4388
4389 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4386.969 kb on + strand, within RR42_RS20275 at 4386.993 kb on + strand at 4386.994 kb on - strand at 4387.109 kb on + strand at 4387.110 kb on - strand at 4387.146 kb on + strand at 4387.147 kb on - strand at 4387.284 kb on - strand, within RR42_RS20280 at 4387.284 kb on - strand, within RR42_RS20280 at 4387.344 kb on - strand, within RR42_RS20280 at 4387.545 kb on - strand, within RR42_RS20280 at 4387.545 kb on - strand, within RR42_RS20280 at 4387.665 kb on - strand, within RR42_RS20280 at 4387.965 kb on + strand at 4387.966 kb on - strand at 4387.966 kb on - strand at 4387.967 kb on + strand at 4387.967 kb on + strand at 4387.967 kb on + strand at 4387.967 kb on + strand at 4387.968 kb on - strand at 4387.996 kb on + strand at 4387.997 kb on - strand at 4388.007 kb on - strand, within RR42_RS20285 at 4388.042 kb on - strand, within RR42_RS20285 at 4388.360 kb on + strand, within RR42_RS20285 at 4388.361 kb on - strand, within RR42_RS20285 at 4388.526 kb on + strand, within RR42_RS20285 at 4388.526 kb on + strand, within RR42_RS20285 at 4388.527 kb on - strand, within RR42_RS20285 at 4388.852 kb on + strand at 4388.852 kb on + strand at 4388.853 kb on - strand at 4388.856 kb on + strand at 4388.856 kb on + strand at 4388.857 kb on - strand at 4388.857 kb on - strand at 4388.869 kb on + strand at 4388.870 kb on - strand at 4388.972 kb on + strand at 4388.972 kb on + strand at 4388.972 kb on + strand at 4388.972 kb on + strand at 4388.973 kb on - strand at 4389.082 kb on + strand at 4389.082 kb on + strand at 4389.082 kb on + strand at 4389.558 kb on - strand, within RR42_RS20290 at 4389.558 kb on - strand, within RR42_RS20290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 4,386,969 + RR42_RS20275 0.89 +0.9 4,386,993 + -0.4 4,386,994 - -1.7 4,387,109 + +0.7 4,387,110 - +0.8 4,387,146 + +1.1 4,387,147 - +0.7 4,387,284 - RR42_RS20280 0.13 -0.7 4,387,284 - RR42_RS20280 0.13 +0.1 4,387,344 - RR42_RS20280 0.21 -1.8 4,387,545 - RR42_RS20280 0.49 +0.2 4,387,545 - RR42_RS20280 0.49 +1.2 4,387,665 - RR42_RS20280 0.65 -0.6 4,387,965 + +0.2 4,387,966 - -0.3 4,387,966 - -0.7 4,387,967 + -0.6 4,387,967 + +0.3 4,387,967 + +0.5 4,387,967 + -3.4 4,387,968 - +0.1 4,387,996 + -0.0 4,387,997 - +0.8 4,388,007 - RR42_RS20285 0.10 -1.5 4,388,042 - RR42_RS20285 0.15 +1.7 4,388,360 + RR42_RS20285 0.56 -1.6 4,388,361 - RR42_RS20285 0.56 -0.5 4,388,526 + RR42_RS20285 0.77 -1.2 4,388,526 + RR42_RS20285 0.77 +0.4 4,388,527 - RR42_RS20285 0.77 -0.7 4,388,852 + +0.2 4,388,852 + -1.6 4,388,853 - -1.2 4,388,856 + +2.2 4,388,856 + +0.1 4,388,857 - +0.7 4,388,857 - +0.2 4,388,869 + -0.1 4,388,870 - -0.6 4,388,972 + -1.2 4,388,972 + +0.6 4,388,972 + +0.7 4,388,972 + -1.0 4,388,973 - -1.1 4,389,082 + -0.2 4,389,082 + +0.3 4,389,082 + +0.5 4,389,558 - RR42_RS20290 0.28 +1.1 4,389,558 - RR42_RS20290 0.28 -0.9
Or see this region's nucleotide sequence