Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19810 and RR42_RS19815 overlap by 4 nucleotides RR42_RS19815 and RR42_RS19820 are separated by 67 nucleotides RR42_RS19820 and RR42_RS19825 overlap by 4 nucleotides
RR42_RS19810: RR42_RS19810 - long-chain fatty acid--CoA ligase, at 4,275,526 to 4,277,232
_RS19810
RR42_RS19815: RR42_RS19815 - enoyl-CoA hydratase, at 4,277,229 to 4,278,047
_RS19815
RR42_RS19820: RR42_RS19820 - membrane protein, at 4,278,115 to 4,278,498
_RS19820
RR42_RS19825: RR42_RS19825 - LysR family transcriptional regulator, at 4,278,495 to 4,279,412
_RS19825
Position (kb)
4278
4279 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4277.179 kb on + strand at 4277.179 kb on + strand at 4277.179 kb on + strand at 4277.179 kb on + strand at 4277.180 kb on - strand at 4277.180 kb on - strand at 4277.228 kb on + strand at 4277.228 kb on + strand at 4277.228 kb on + strand at 4277.229 kb on - strand at 4277.229 kb on - strand at 4277.229 kb on - strand at 4278.046 kb on - strand at 4278.046 kb on - strand at 4278.155 kb on + strand, within RR42_RS19820 at 4278.240 kb on - strand, within RR42_RS19820 at 4278.258 kb on - strand, within RR42_RS19820 at 4278.281 kb on + strand, within RR42_RS19820 at 4278.281 kb on + strand, within RR42_RS19820 at 4278.447 kb on + strand, within RR42_RS19820 at 4278.448 kb on - strand, within RR42_RS19820 at 4278.558 kb on - strand at 4278.805 kb on + strand, within RR42_RS19825 at 4278.997 kb on + strand, within RR42_RS19825 at 4278.997 kb on + strand, within RR42_RS19825 at 4279.383 kb on - strand at 4279.401 kb on + strand at 4279.402 kb on - strand at 4279.475 kb on - strand at 4279.497 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 4,277,179 + -0.3 4,277,179 + +0.3 4,277,179 + -2.4 4,277,179 + +0.2 4,277,180 - +1.2 4,277,180 - +0.8 4,277,228 + -0.1 4,277,228 + -0.7 4,277,228 + +2.6 4,277,229 - +0.4 4,277,229 - +0.9 4,277,229 - -2.1 4,278,046 - -0.5 4,278,046 - +1.2 4,278,155 + RR42_RS19820 0.10 +0.4 4,278,240 - RR42_RS19820 0.33 -4.4 4,278,258 - RR42_RS19820 0.37 -2.9 4,278,281 + RR42_RS19820 0.43 +0.0 4,278,281 + RR42_RS19820 0.43 -0.4 4,278,447 + RR42_RS19820 0.86 -2.2 4,278,448 - RR42_RS19820 0.87 -1.4 4,278,558 - +0.9 4,278,805 + RR42_RS19825 0.34 +0.0 4,278,997 + RR42_RS19825 0.55 +0.1 4,278,997 + RR42_RS19825 0.55 +2.1 4,279,383 - -1.2 4,279,401 + +1.0 4,279,402 - -0.2 4,279,475 - +0.6 4,279,497 + -0.9
Or see this region's nucleotide sequence