Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18825 and RR42_RS18830 are separated by 212 nucleotides RR42_RS18830 and RR42_RS18835 are separated by 60 nucleotides RR42_RS18835 and RR42_RS18840 are separated by 19 nucleotides
RR42_RS18825: RR42_RS18825 - ubiquinol-cytochrome C reductase, at 4,082,250 to 4,082,867
_RS18825
RR42_RS18830: RR42_RS18830 - large-conductance mechanosensitive channel, at 4,083,080 to 4,083,511
_RS18830
RR42_RS18835: RR42_RS18835 - Nif3-like dinuclear metal center hexameric protein, at 4,083,572 to 4,084,318
_RS18835
RR42_RS18840: RR42_RS18840 - 2-alkenal reductase, at 4,084,338 to 4,085,531
_RS18840
Position (kb)
4083
4084
4085 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4082.709 kb on + strand, within RR42_RS18825 at 4082.754 kb on - strand, within RR42_RS18825 at 4082.892 kb on + strand at 4082.893 kb on - strand at 4082.911 kb on - strand at 4082.918 kb on - strand at 4082.918 kb on - strand at 4082.923 kb on - strand at 4082.923 kb on - strand at 4082.923 kb on - strand at 4082.923 kb on - strand at 4083.001 kb on + strand at 4083.002 kb on - strand at 4083.002 kb on - strand at 4083.002 kb on - strand at 4083.002 kb on - strand at 4083.002 kb on - strand at 4083.002 kb on - strand at 4083.002 kb on - strand at 4083.037 kb on + strand at 4083.038 kb on - strand at 4083.038 kb on - strand at 4083.038 kb on - strand at 4083.223 kb on + strand, within RR42_RS18830 at 4083.304 kb on + strand, within RR42_RS18830 at 4083.305 kb on - strand, within RR42_RS18830 at 4083.305 kb on - strand, within RR42_RS18830 at 4083.349 kb on + strand, within RR42_RS18830 at 4083.349 kb on + strand, within RR42_RS18830 at 4083.349 kb on + strand, within RR42_RS18830 at 4083.350 kb on - strand, within RR42_RS18830 at 4083.350 kb on - strand, within RR42_RS18830 at 4083.350 kb on - strand, within RR42_RS18830 at 4083.590 kb on - strand at 4083.602 kb on - strand at 4083.602 kb on - strand at 4083.747 kb on + strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.783 kb on + strand, within RR42_RS18835 at 4084.032 kb on + strand, within RR42_RS18835 at 4084.033 kb on - strand, within RR42_RS18835 at 4084.033 kb on - strand, within RR42_RS18835 at 4084.033 kb on - strand, within RR42_RS18835 at 4084.214 kb on + strand, within RR42_RS18835 at 4084.215 kb on - strand, within RR42_RS18835 at 4084.215 kb on - strand, within RR42_RS18835 at 4084.254 kb on + strand at 4084.254 kb on + strand at 4084.254 kb on + strand at 4084.255 kb on - strand at 4084.255 kb on - strand at 4084.263 kb on + strand at 4084.264 kb on - strand at 4084.294 kb on - strand at 4084.294 kb on - strand at 4084.294 kb on - strand at 4084.319 kb on - strand at 4084.319 kb on - strand at 4084.337 kb on + strand at 4084.337 kb on + strand at 4084.524 kb on + strand, within RR42_RS18840 at 4084.713 kb on + strand, within RR42_RS18840 at 4084.720 kb on + strand, within RR42_RS18840 at 4084.877 kb on + strand, within RR42_RS18840 at 4085.101 kb on - strand, within RR42_RS18840 at 4085.101 kb on - strand, within RR42_RS18840 at 4085.101 kb on - strand, within RR42_RS18840
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 4,082,709 + RR42_RS18825 0.74 +0.7 4,082,754 - RR42_RS18825 0.82 +0.2 4,082,892 + -2.1 4,082,893 - +1.7 4,082,911 - -0.4 4,082,918 - +1.7 4,082,918 - -0.2 4,082,923 - +0.9 4,082,923 - +0.7 4,082,923 - -0.9 4,082,923 - +0.8 4,083,001 + +0.3 4,083,002 - +0.0 4,083,002 - -0.1 4,083,002 - +0.3 4,083,002 - +0.3 4,083,002 - +1.0 4,083,002 - -1.9 4,083,002 - +0.8 4,083,037 + -0.5 4,083,038 - -1.4 4,083,038 - -0.5 4,083,038 - -0.8 4,083,223 + RR42_RS18830 0.33 -1.5 4,083,304 + RR42_RS18830 0.52 +0.3 4,083,305 - RR42_RS18830 0.52 -1.4 4,083,305 - RR42_RS18830 0.52 -0.3 4,083,349 + RR42_RS18830 0.62 -1.3 4,083,349 + RR42_RS18830 0.62 -0.4 4,083,349 + RR42_RS18830 0.62 -3.1 4,083,350 - RR42_RS18830 0.62 -1.2 4,083,350 - RR42_RS18830 0.62 -3.0 4,083,350 - RR42_RS18830 0.62 +0.0 4,083,590 - +0.8 4,083,602 - -1.7 4,083,602 - -0.7 4,083,747 + RR42_RS18835 0.23 +0.1 4,083,748 - RR42_RS18835 0.24 -2.0 4,083,748 - RR42_RS18835 0.24 -1.9 4,083,748 - RR42_RS18835 0.24 -2.1 4,083,748 - RR42_RS18835 0.24 -1.7 4,083,748 - RR42_RS18835 0.24 -1.7 4,083,783 + RR42_RS18835 0.28 -1.2 4,084,032 + RR42_RS18835 0.62 -2.1 4,084,033 - RR42_RS18835 0.62 -1.0 4,084,033 - RR42_RS18835 0.62 -0.5 4,084,033 - RR42_RS18835 0.62 +0.5 4,084,214 + RR42_RS18835 0.86 +2.2 4,084,215 - RR42_RS18835 0.86 -2.7 4,084,215 - RR42_RS18835 0.86 -0.1 4,084,254 + -0.2 4,084,254 + -1.1 4,084,254 + +0.1 4,084,255 - +1.3 4,084,255 - +0.7 4,084,263 + +0.7 4,084,264 - -1.7 4,084,294 - +1.5 4,084,294 - -0.6 4,084,294 - -1.1 4,084,319 - +0.7 4,084,319 - -0.7 4,084,337 + -2.0 4,084,337 + +1.5 4,084,524 + RR42_RS18840 0.16 -1.2 4,084,713 + RR42_RS18840 0.31 -1.5 4,084,720 + RR42_RS18840 0.32 -1.8 4,084,877 + RR42_RS18840 0.45 -0.2 4,085,101 - RR42_RS18840 0.64 -0.3 4,085,101 - RR42_RS18840 0.64 +0.7 4,085,101 - RR42_RS18840 0.64 -1.4
Or see this region's nucleotide sequence