Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18450 and RR42_RS18455 are separated by 157 nucleotides RR42_RS18455 and RR42_RS18460 are separated by 68 nucleotides RR42_RS18460 and RR42_RS18465 are separated by 253 nucleotides
RR42_RS18450: RR42_RS18450 - hypothetical protein, at 3,995,047 to 3,996,102
_RS18450
RR42_RS18455: RR42_RS18455 - S-adenosylhomocysteine nucleosidase, at 3,996,260 to 3,997,027
_RS18455
RR42_RS18460: RR42_RS18460 - membrane protein, at 3,997,096 to 3,997,332
_RS18460
RR42_RS18465: RR42_RS18465 - glutamyl-tRNA reductase, at 3,997,586 to 3,998,890
_RS18465
Position (kb)
3997
3998 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3996.122 kb on + strand at 3996.122 kb on + strand at 3996.185 kb on + strand at 3996.223 kb on + strand at 3996.223 kb on + strand at 3996.272 kb on - strand at 3996.369 kb on - strand, within RR42_RS18455 at 3996.369 kb on - strand, within RR42_RS18455 at 3996.369 kb on - strand, within RR42_RS18455 at 3996.386 kb on + strand, within RR42_RS18455 at 3996.646 kb on + strand, within RR42_RS18455 at 3996.646 kb on + strand, within RR42_RS18455 at 3996.646 kb on + strand, within RR42_RS18455 at 3996.678 kb on - strand, within RR42_RS18455 at 3996.884 kb on + strand, within RR42_RS18455 at 3996.885 kb on - strand, within RR42_RS18455 at 3996.885 kb on - strand, within RR42_RS18455 at 3996.983 kb on + strand at 3996.984 kb on - strand at 3997.055 kb on + strand at 3997.056 kb on - strand at 3997.208 kb on + strand, within RR42_RS18460 at 3997.209 kb on - strand, within RR42_RS18460 at 3997.355 kb on + strand at 3997.356 kb on - strand at 3997.356 kb on - strand at 3997.408 kb on - strand at 3997.408 kb on - strand at 3997.451 kb on + strand at 3997.451 kb on + strand at 3997.451 kb on + strand at 3997.453 kb on + strand at 3997.453 kb on + strand at 3997.453 kb on + strand at 3997.453 kb on + strand at 3997.458 kb on + strand at 3997.458 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,996,122 + +0.0 3,996,122 + +0.9 3,996,185 + +0.3 3,996,223 + +0.0 3,996,223 + +0.3 3,996,272 - -1.7 3,996,369 - RR42_RS18455 0.14 -2.7 3,996,369 - RR42_RS18455 0.14 -0.7 3,996,369 - RR42_RS18455 0.14 +1.6 3,996,386 + RR42_RS18455 0.16 +1.6 3,996,646 + RR42_RS18455 0.50 -1.2 3,996,646 + RR42_RS18455 0.50 -1.9 3,996,646 + RR42_RS18455 0.50 -0.5 3,996,678 - RR42_RS18455 0.54 +0.3 3,996,884 + RR42_RS18455 0.81 +4.1 3,996,885 - RR42_RS18455 0.81 -0.3 3,996,885 - RR42_RS18455 0.81 +0.3 3,996,983 + -0.4 3,996,984 - -1.6 3,997,055 + -0.3 3,997,056 - +0.3 3,997,208 + RR42_RS18460 0.47 -1.3 3,997,209 - RR42_RS18460 0.48 -0.3 3,997,355 + -0.3 3,997,356 - +1.3 3,997,356 - +0.6 3,997,408 - -0.1 3,997,408 - -0.1 3,997,451 + -1.4 3,997,451 + +4.0 3,997,451 + -0.7 3,997,453 + +0.8 3,997,453 + -0.5 3,997,453 + -1.0 3,997,453 + -1.5 3,997,458 + +0.6 3,997,458 + +0.1
Or see this region's nucleotide sequence