Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18305 and RR42_RS18310 are separated by 35 nucleotides RR42_RS18310 and RR42_RS18315 are separated by 26 nucleotides RR42_RS18315 and RR42_RS18320 are separated by 54 nucleotides
RR42_RS18305: RR42_RS18305 - anthranilate phosphoribosyltransferase, at 3,965,362 to 3,966,390
_RS18305
RR42_RS18310: RR42_RS18310 - indole-3-glycerol-phosphate synthase, at 3,966,426 to 3,967,229
_RS18310
RR42_RS18315: RR42_RS18315 - toxin, at 3,967,256 to 3,967,897
_RS18315
RR42_RS18320: RR42_RS18320 - uracil-DNA glycosylase, at 3,967,952 to 3,968,755
_RS18320
Position (kb)
3966
3967
3968 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3965.901 kb on + strand, within RR42_RS18305 at 3965.902 kb on - strand, within RR42_RS18305 at 3965.902 kb on - strand, within RR42_RS18305 at 3966.028 kb on + strand, within RR42_RS18305 at 3966.388 kb on + strand at 3966.420 kb on + strand at 3966.687 kb on + strand, within RR42_RS18310 at 3966.831 kb on + strand, within RR42_RS18310 at 3966.832 kb on - strand, within RR42_RS18310 at 3967.240 kb on + strand at 3967.241 kb on - strand at 3967.241 kb on - strand at 3967.241 kb on - strand at 3967.354 kb on + strand, within RR42_RS18315 at 3967.354 kb on + strand, within RR42_RS18315 at 3967.354 kb on + strand, within RR42_RS18315 at 3967.355 kb on - strand, within RR42_RS18315 at 3967.355 kb on - strand, within RR42_RS18315 at 3967.355 kb on - strand, within RR42_RS18315 at 3967.355 kb on - strand, within RR42_RS18315 at 3967.371 kb on - strand, within RR42_RS18315 at 3967.371 kb on - strand, within RR42_RS18315 at 3967.941 kb on - strand at 3967.941 kb on - strand at 3967.941 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,965,901 + RR42_RS18305 0.52 -1.5 3,965,902 - RR42_RS18305 0.52 -3.2 3,965,902 - RR42_RS18305 0.52 -1.1 3,966,028 + RR42_RS18305 0.65 -0.5 3,966,388 + +0.2 3,966,420 + -0.1 3,966,687 + RR42_RS18310 0.32 -1.3 3,966,831 + RR42_RS18310 0.50 -2.5 3,966,832 - RR42_RS18310 0.50 -0.3 3,967,240 + -0.0 3,967,241 - -0.1 3,967,241 - +0.0 3,967,241 - +0.9 3,967,354 + RR42_RS18315 0.15 -2.3 3,967,354 + RR42_RS18315 0.15 -0.7 3,967,354 + RR42_RS18315 0.15 -0.5 3,967,355 - RR42_RS18315 0.15 +2.6 3,967,355 - RR42_RS18315 0.15 +0.0 3,967,355 - RR42_RS18315 0.15 +0.3 3,967,355 - RR42_RS18315 0.15 +0.3 3,967,371 - RR42_RS18315 0.18 +0.6 3,967,371 - RR42_RS18315 0.18 -1.0 3,967,941 - +0.8 3,967,941 - +0.7 3,967,941 - +0.8
Or see this region's nucleotide sequence