Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18290 and RR42_RS18295 are separated by 461 nucleotides RR42_RS18295 and RR42_RS18300 are separated by 24 nucleotides RR42_RS18300 and RR42_RS18305 are separated by 68 nucleotides
RR42_RS18290: RR42_RS18290 - phosphoglycolate phosphatase, at 3,961,989 to 3,962,720
_RS18290
RR42_RS18295: RR42_RS18295 - anthranilate synthase component I, at 3,963,182 to 3,964,699
_RS18295
RR42_RS18300: RR42_RS18300 - anthranilate synthase subunit II, at 3,964,724 to 3,965,293
_RS18300
RR42_RS18305: RR42_RS18305 - anthranilate phosphoribosyltransferase, at 3,965,362 to 3,966,390
_RS18305
Position (kb)
3963
3964
3965 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3962.277 kb on + strand, within RR42_RS18290 at 3962.277 kb on + strand, within RR42_RS18290 at 3962.277 kb on + strand, within RR42_RS18290 at 3962.278 kb on - strand, within RR42_RS18290 at 3962.278 kb on - strand, within RR42_RS18290 at 3962.289 kb on + strand, within RR42_RS18290 at 3962.289 kb on + strand, within RR42_RS18290 at 3962.290 kb on - strand, within RR42_RS18290 at 3962.290 kb on - strand, within RR42_RS18290 at 3962.322 kb on + strand, within RR42_RS18290 at 3962.457 kb on + strand, within RR42_RS18290 at 3962.457 kb on + strand, within RR42_RS18290 at 3962.457 kb on + strand, within RR42_RS18290 at 3962.458 kb on - strand, within RR42_RS18290 at 3962.458 kb on - strand, within RR42_RS18290 at 3962.619 kb on + strand, within RR42_RS18290 at 3962.626 kb on - strand, within RR42_RS18290 at 3962.626 kb on - strand, within RR42_RS18290 at 3962.626 kb on - strand, within RR42_RS18290 at 3962.644 kb on + strand, within RR42_RS18290 at 3962.644 kb on + strand, within RR42_RS18290 at 3962.645 kb on - strand, within RR42_RS18290 at 3962.645 kb on - strand, within RR42_RS18290 at 3962.645 kb on - strand, within RR42_RS18290 at 3962.666 kb on + strand at 3962.666 kb on + strand at 3962.666 kb on + strand at 3962.666 kb on + strand at 3962.667 kb on - strand at 3962.667 kb on - strand at 3962.667 kb on - strand at 3962.668 kb on + strand at 3962.669 kb on - strand at 3962.881 kb on - strand at 3963.144 kb on + strand at 3963.144 kb on + strand at 3963.365 kb on + strand, within RR42_RS18295 at 3963.555 kb on - strand, within RR42_RS18295 at 3963.578 kb on + strand, within RR42_RS18295 at 3963.579 kb on - strand, within RR42_RS18295 at 3963.579 kb on - strand, within RR42_RS18295 at 3963.579 kb on - strand, within RR42_RS18295 at 3963.579 kb on - strand, within RR42_RS18295 at 3963.579 kb on - strand, within RR42_RS18295 at 3963.580 kb on + strand, within RR42_RS18295 at 3963.580 kb on + strand, within RR42_RS18295 at 3963.580 kb on + strand, within RR42_RS18295 at 3963.581 kb on - strand, within RR42_RS18295 at 3963.581 kb on - strand, within RR42_RS18295 at 3963.692 kb on + strand, within RR42_RS18295 at 3963.692 kb on + strand, within RR42_RS18295 at 3963.827 kb on + strand, within RR42_RS18295 at 3963.851 kb on + strand, within RR42_RS18295 at 3963.881 kb on + strand, within RR42_RS18295 at 3963.881 kb on + strand, within RR42_RS18295 at 3963.882 kb on - strand, within RR42_RS18295 at 3963.882 kb on - strand, within RR42_RS18295 at 3963.882 kb on - strand, within RR42_RS18295 at 3963.962 kb on + strand, within RR42_RS18295 at 3963.995 kb on + strand, within RR42_RS18295 at 3963.995 kb on + strand, within RR42_RS18295 at 3963.995 kb on + strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3964.001 kb on + strand, within RR42_RS18295 at 3964.001 kb on + strand, within RR42_RS18295 at 3964.001 kb on + strand, within RR42_RS18295 at 3964.001 kb on + strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.007 kb on + strand, within RR42_RS18295 at 3964.310 kb on + strand, within RR42_RS18295 at 3964.310 kb on + strand, within RR42_RS18295 at 3964.310 kb on + strand, within RR42_RS18295 at 3964.310 kb on + strand, within RR42_RS18295 at 3964.311 kb on - strand, within RR42_RS18295 at 3964.479 kb on - strand, within RR42_RS18295 at 3964.496 kb on + strand, within RR42_RS18295 at 3964.497 kb on - strand, within RR42_RS18295 at 3964.568 kb on + strand at 3964.568 kb on + strand at 3964.569 kb on - strand at 3964.569 kb on - strand at 3964.569 kb on - strand at 3964.569 kb on - strand at 3964.694 kb on - strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.698 kb on - strand at 3964.698 kb on - strand at 3964.698 kb on - strand at 3964.698 kb on - strand at 3964.703 kb on - strand at 3964.745 kb on + strand at 3964.746 kb on - strand at 3964.746 kb on - strand at 3964.761 kb on - strand at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.862 kb on - strand, within RR42_RS18300 at 3964.862 kb on - strand, within RR42_RS18300 at 3965.103 kb on - strand, within RR42_RS18300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,962,277 + RR42_RS18290 0.39 +0.2 3,962,277 + RR42_RS18290 0.39 -0.2 3,962,277 + RR42_RS18290 0.39 -0.0 3,962,278 - RR42_RS18290 0.39 -0.3 3,962,278 - RR42_RS18290 0.39 +2.3 3,962,289 + RR42_RS18290 0.41 -0.3 3,962,289 + RR42_RS18290 0.41 -0.8 3,962,290 - RR42_RS18290 0.41 +0.3 3,962,290 - RR42_RS18290 0.41 +1.5 3,962,322 + RR42_RS18290 0.45 +1.0 3,962,457 + RR42_RS18290 0.64 -2.5 3,962,457 + RR42_RS18290 0.64 -1.7 3,962,457 + RR42_RS18290 0.64 -0.2 3,962,458 - RR42_RS18290 0.64 +1.3 3,962,458 - RR42_RS18290 0.64 +0.6 3,962,619 + RR42_RS18290 0.86 -1.0 3,962,626 - RR42_RS18290 0.87 -1.5 3,962,626 - RR42_RS18290 0.87 -1.3 3,962,626 - RR42_RS18290 0.87 +0.9 3,962,644 + RR42_RS18290 0.89 -0.0 3,962,644 + RR42_RS18290 0.89 -0.9 3,962,645 - RR42_RS18290 0.90 +1.1 3,962,645 - RR42_RS18290 0.90 +0.0 3,962,645 - RR42_RS18290 0.90 -1.7 3,962,666 + -0.5 3,962,666 + -0.0 3,962,666 + -0.2 3,962,666 + -0.9 3,962,667 - -0.5 3,962,667 - +0.3 3,962,667 - -0.8 3,962,668 + -0.7 3,962,669 - -1.5 3,962,881 - -1.5 3,963,144 + -0.1 3,963,144 + -0.9 3,963,365 + RR42_RS18295 0.12 -0.2 3,963,555 - RR42_RS18295 0.25 -3.3 3,963,578 + RR42_RS18295 0.26 -2.2 3,963,579 - RR42_RS18295 0.26 -3.0 3,963,579 - RR42_RS18295 0.26 -2.2 3,963,579 - RR42_RS18295 0.26 -1.0 3,963,579 - RR42_RS18295 0.26 -2.5 3,963,579 - RR42_RS18295 0.26 -1.9 3,963,580 + RR42_RS18295 0.26 -2.0 3,963,580 + RR42_RS18295 0.26 +0.6 3,963,580 + RR42_RS18295 0.26 +1.6 3,963,581 - RR42_RS18295 0.26 -1.0 3,963,581 - RR42_RS18295 0.26 -0.5 3,963,692 + RR42_RS18295 0.34 +0.2 3,963,692 + RR42_RS18295 0.34 -1.7 3,963,827 + RR42_RS18295 0.42 -1.0 3,963,851 + RR42_RS18295 0.44 +0.3 3,963,881 + RR42_RS18295 0.46 +1.3 3,963,881 + RR42_RS18295 0.46 -1.8 3,963,882 - RR42_RS18295 0.46 -2.4 3,963,882 - RR42_RS18295 0.46 -1.9 3,963,882 - RR42_RS18295 0.46 -2.2 3,963,962 + RR42_RS18295 0.51 -3.5 3,963,995 + RR42_RS18295 0.54 -3.0 3,963,995 + RR42_RS18295 0.54 -1.0 3,963,995 + RR42_RS18295 0.54 +0.4 3,963,996 - RR42_RS18295 0.54 -2.5 3,963,996 - RR42_RS18295 0.54 -2.5 3,963,996 - RR42_RS18295 0.54 -3.0 3,963,996 - RR42_RS18295 0.54 -0.6 3,963,996 - RR42_RS18295 0.54 -2.2 3,964,001 + RR42_RS18295 0.54 +1.3 3,964,001 + RR42_RS18295 0.54 -3.7 3,964,001 + RR42_RS18295 0.54 +0.2 3,964,001 + RR42_RS18295 0.54 -2.3 3,964,002 - RR42_RS18295 0.54 -3.6 3,964,002 - RR42_RS18295 0.54 +0.1 3,964,002 - RR42_RS18295 0.54 -1.9 3,964,002 - RR42_RS18295 0.54 -0.7 3,964,002 - RR42_RS18295 0.54 -1.8 3,964,007 + RR42_RS18295 0.54 -1.6 3,964,310 + RR42_RS18295 0.74 +0.3 3,964,310 + RR42_RS18295 0.74 -1.5 3,964,310 + RR42_RS18295 0.74 -3.1 3,964,310 + RR42_RS18295 0.74 -3.4 3,964,311 - RR42_RS18295 0.74 -0.6 3,964,479 - RR42_RS18295 0.85 -1.3 3,964,496 + RR42_RS18295 0.87 -2.6 3,964,497 - RR42_RS18295 0.87 -0.6 3,964,568 + -0.3 3,964,568 + -0.2 3,964,569 - +0.3 3,964,569 - -1.2 3,964,569 - -1.2 3,964,569 - -2.3 3,964,694 - -2.1 3,964,697 + +0.6 3,964,697 + +1.8 3,964,697 + +1.3 3,964,697 + -1.7 3,964,697 + -0.1 3,964,697 + -1.8 3,964,697 + +0.1 3,964,698 - -1.8 3,964,698 - +0.8 3,964,698 - -2.5 3,964,698 - -0.7 3,964,703 - -3.4 3,964,745 + -2.2 3,964,746 - -2.0 3,964,746 - -1.1 3,964,761 - -1.8 3,964,861 + RR42_RS18300 0.24 -1.1 3,964,861 + RR42_RS18300 0.24 -2.5 3,964,861 + RR42_RS18300 0.24 -3.0 3,964,861 + RR42_RS18300 0.24 -1.3 3,964,861 + RR42_RS18300 0.24 -1.0 3,964,862 - RR42_RS18300 0.24 -1.2 3,964,862 - RR42_RS18300 0.24 -0.4 3,965,103 - RR42_RS18300 0.66 -2.4
Or see this region's nucleotide sequence