Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18255 and RR42_RS18260 are separated by 59 nucleotides RR42_RS18260 and RR42_RS18265 are separated by 57 nucleotides RR42_RS18265 and RR42_RS18270 are separated by 63 nucleotides
RR42_RS18255: RR42_RS18255 - enoyl-CoA hydratase, at 3,956,531 to 3,957,301
_RS18255
RR42_RS18260: RR42_RS18260 - phenylacetic acid degradation protein, at 3,957,361 to 3,957,807
_RS18260
RR42_RS18265: RR42_RS18265 - phenylacetate--CoA ligase, at 3,957,865 to 3,959,166
_RS18265
RR42_RS18270: RR42_RS18270 - murein transglycosylase, at 3,959,230 to 3,960,399
_RS18270
Position (kb)
3957
3958
3959
3960 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3957.161 kb on + strand, within RR42_RS18255 at 3957.161 kb on + strand, within RR42_RS18255 at 3957.162 kb on - strand, within RR42_RS18255 at 3957.162 kb on - strand, within RR42_RS18255 at 3957.409 kb on + strand, within RR42_RS18260 at 3957.409 kb on + strand, within RR42_RS18260 at 3957.409 kb on + strand, within RR42_RS18260 at 3957.410 kb on - strand, within RR42_RS18260 at 3957.410 kb on - strand, within RR42_RS18260 at 3957.410 kb on - strand, within RR42_RS18260 at 3957.410 kb on - strand, within RR42_RS18260 at 3957.410 kb on - strand, within RR42_RS18260 at 3957.588 kb on + strand, within RR42_RS18260 at 3957.589 kb on - strand, within RR42_RS18260 at 3957.589 kb on - strand, within RR42_RS18260 at 3957.589 kb on - strand, within RR42_RS18260 at 3957.594 kb on + strand, within RR42_RS18260 at 3957.594 kb on + strand, within RR42_RS18260 at 3957.595 kb on - strand, within RR42_RS18260 at 3957.700 kb on + strand, within RR42_RS18260 at 3957.700 kb on + strand, within RR42_RS18260 at 3957.700 kb on + strand, within RR42_RS18260 at 3957.700 kb on + strand, within RR42_RS18260 at 3957.700 kb on + strand, within RR42_RS18260 at 3957.700 kb on + strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.701 kb on - strand, within RR42_RS18260 at 3957.825 kb on + strand at 3957.825 kb on + strand at 3957.825 kb on + strand at 3957.826 kb on - strand at 3957.826 kb on - strand at 3957.831 kb on + strand at 3957.831 kb on + strand at 3957.831 kb on + strand at 3957.831 kb on + strand at 3957.831 kb on + strand at 3957.832 kb on - strand at 3957.832 kb on - strand at 3957.832 kb on - strand at 3957.843 kb on + strand at 3957.844 kb on - strand at 3957.844 kb on - strand at 3957.844 kb on - strand at 3957.844 kb on - strand at 3957.844 kb on - strand at 3957.970 kb on + strand at 3957.971 kb on - strand at 3957.971 kb on - strand at 3957.988 kb on + strand at 3957.988 kb on + strand at 3957.989 kb on - strand at 3958.089 kb on + strand, within RR42_RS18265 at 3958.089 kb on + strand, within RR42_RS18265 at 3958.089 kb on + strand, within RR42_RS18265 at 3958.089 kb on + strand, within RR42_RS18265 at 3958.089 kb on + strand, within RR42_RS18265 at 3958.090 kb on - strand, within RR42_RS18265 at 3958.090 kb on - strand, within RR42_RS18265 at 3958.093 kb on + strand, within RR42_RS18265 at 3958.183 kb on + strand, within RR42_RS18265 at 3958.184 kb on - strand, within RR42_RS18265 at 3958.269 kb on + strand, within RR42_RS18265 at 3958.269 kb on + strand, within RR42_RS18265 at 3958.270 kb on - strand, within RR42_RS18265 at 3958.270 kb on - strand, within RR42_RS18265 at 3958.276 kb on + strand, within RR42_RS18265 at 3958.282 kb on + strand, within RR42_RS18265 at 3958.293 kb on + strand, within RR42_RS18265 at 3958.294 kb on - strand, within RR42_RS18265 at 3958.294 kb on - strand, within RR42_RS18265 at 3958.315 kb on + strand, within RR42_RS18265 at 3958.316 kb on - strand, within RR42_RS18265 at 3958.316 kb on - strand, within RR42_RS18265 at 3958.316 kb on - strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.429 kb on + strand, within RR42_RS18265 at 3958.430 kb on - strand, within RR42_RS18265 at 3958.430 kb on - strand, within RR42_RS18265 at 3958.430 kb on - strand, within RR42_RS18265 at 3958.430 kb on - strand, within RR42_RS18265 at 3958.430 kb on - strand, within RR42_RS18265 at 3958.430 kb on - strand, within RR42_RS18265 at 3958.430 kb on - strand, within RR42_RS18265 at 3958.430 kb on - strand, within RR42_RS18265 at 3958.430 kb on - strand, within RR42_RS18265 at 3958.576 kb on - strand, within RR42_RS18265 at 3958.579 kb on + strand, within RR42_RS18265 at 3958.667 kb on - strand, within RR42_RS18265 at 3958.734 kb on + strand, within RR42_RS18265 at 3958.942 kb on + strand, within RR42_RS18265 at 3958.942 kb on + strand, within RR42_RS18265 at 3958.943 kb on - strand, within RR42_RS18265 at 3959.053 kb on + strand at 3959.053 kb on + strand at 3959.054 kb on - strand at 3959.103 kb on + strand at 3959.511 kb on + strand, within RR42_RS18270 at 3959.511 kb on + strand, within RR42_RS18270 at 3959.511 kb on + strand, within RR42_RS18270 at 3959.515 kb on - strand, within RR42_RS18270 at 3959.876 kb on + strand, within RR42_RS18270 at 3959.877 kb on - strand, within RR42_RS18270 at 3959.933 kb on + strand, within RR42_RS18270 at 3960.008 kb on + strand, within RR42_RS18270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,957,161 + RR42_RS18255 0.82 -0.6 3,957,161 + RR42_RS18255 0.82 -0.3 3,957,162 - RR42_RS18255 0.82 -0.3 3,957,162 - RR42_RS18255 0.82 -0.8 3,957,409 + RR42_RS18260 0.11 -0.3 3,957,409 + RR42_RS18260 0.11 -0.1 3,957,409 + RR42_RS18260 0.11 +1.3 3,957,410 - RR42_RS18260 0.11 +0.2 3,957,410 - RR42_RS18260 0.11 +0.3 3,957,410 - RR42_RS18260 0.11 +0.6 3,957,410 - RR42_RS18260 0.11 +3.3 3,957,410 - RR42_RS18260 0.11 -1.4 3,957,588 + RR42_RS18260 0.51 +0.4 3,957,589 - RR42_RS18260 0.51 +0.7 3,957,589 - RR42_RS18260 0.51 -2.0 3,957,589 - RR42_RS18260 0.51 +2.2 3,957,594 + RR42_RS18260 0.52 -0.0 3,957,594 + RR42_RS18260 0.52 -0.1 3,957,595 - RR42_RS18260 0.52 +1.7 3,957,700 + RR42_RS18260 0.76 -0.0 3,957,700 + RR42_RS18260 0.76 +1.7 3,957,700 + RR42_RS18260 0.76 -1.7 3,957,700 + RR42_RS18260 0.76 +1.5 3,957,700 + RR42_RS18260 0.76 -1.3 3,957,700 + RR42_RS18260 0.76 -0.9 3,957,701 - RR42_RS18260 0.76 +0.0 3,957,701 - RR42_RS18260 0.76 -0.2 3,957,701 - RR42_RS18260 0.76 -0.3 3,957,701 - RR42_RS18260 0.76 -2.3 3,957,701 - RR42_RS18260 0.76 -1.5 3,957,701 - RR42_RS18260 0.76 -2.2 3,957,701 - RR42_RS18260 0.76 -0.9 3,957,701 - RR42_RS18260 0.76 +0.2 3,957,701 - RR42_RS18260 0.76 +0.3 3,957,701 - RR42_RS18260 0.76 +0.0 3,957,825 + +1.0 3,957,825 + +1.1 3,957,825 + -0.4 3,957,826 - -1.2 3,957,826 - -0.3 3,957,831 + -0.3 3,957,831 + -1.0 3,957,831 + +0.3 3,957,831 + -0.6 3,957,831 + +0.5 3,957,832 - -0.7 3,957,832 - +0.3 3,957,832 - +0.5 3,957,843 + -2.2 3,957,844 - +1.6 3,957,844 - -1.1 3,957,844 - -0.5 3,957,844 - -0.5 3,957,844 - +0.9 3,957,970 + -0.3 3,957,971 - -0.3 3,957,971 - +0.5 3,957,988 + +2.3 3,957,988 + +0.6 3,957,989 - -0.5 3,958,089 + RR42_RS18265 0.17 -1.5 3,958,089 + RR42_RS18265 0.17 +0.7 3,958,089 + RR42_RS18265 0.17 +0.7 3,958,089 + RR42_RS18265 0.17 +0.1 3,958,089 + RR42_RS18265 0.17 -0.7 3,958,090 - RR42_RS18265 0.17 +0.7 3,958,090 - RR42_RS18265 0.17 -1.7 3,958,093 + RR42_RS18265 0.18 -3.3 3,958,183 + RR42_RS18265 0.24 -0.4 3,958,184 - RR42_RS18265 0.25 +0.8 3,958,269 + RR42_RS18265 0.31 -1.4 3,958,269 + RR42_RS18265 0.31 -0.4 3,958,270 - RR42_RS18265 0.31 +0.8 3,958,270 - RR42_RS18265 0.31 -0.3 3,958,276 + RR42_RS18265 0.32 -2.5 3,958,282 + RR42_RS18265 0.32 -0.6 3,958,293 + RR42_RS18265 0.33 -0.9 3,958,294 - RR42_RS18265 0.33 +0.1 3,958,294 - RR42_RS18265 0.33 -0.6 3,958,315 + RR42_RS18265 0.35 -1.6 3,958,316 - RR42_RS18265 0.35 -0.7 3,958,316 - RR42_RS18265 0.35 -0.5 3,958,316 - RR42_RS18265 0.35 +0.6 3,958,429 + RR42_RS18265 0.43 -0.7 3,958,429 + RR42_RS18265 0.43 +0.7 3,958,429 + RR42_RS18265 0.43 +0.3 3,958,429 + RR42_RS18265 0.43 -0.7 3,958,429 + RR42_RS18265 0.43 -1.8 3,958,429 + RR42_RS18265 0.43 +0.5 3,958,429 + RR42_RS18265 0.43 -0.4 3,958,429 + RR42_RS18265 0.43 +2.2 3,958,429 + RR42_RS18265 0.43 +1.3 3,958,429 + RR42_RS18265 0.43 +3.1 3,958,429 + RR42_RS18265 0.43 -2.2 3,958,430 - RR42_RS18265 0.43 +0.2 3,958,430 - RR42_RS18265 0.43 -0.5 3,958,430 - RR42_RS18265 0.43 -1.1 3,958,430 - RR42_RS18265 0.43 -0.7 3,958,430 - RR42_RS18265 0.43 -1.7 3,958,430 - RR42_RS18265 0.43 +0.7 3,958,430 - RR42_RS18265 0.43 +0.3 3,958,430 - RR42_RS18265 0.43 -1.0 3,958,430 - RR42_RS18265 0.43 +2.1 3,958,576 - RR42_RS18265 0.55 -3.1 3,958,579 + RR42_RS18265 0.55 -1.0 3,958,667 - RR42_RS18265 0.62 +0.0 3,958,734 + RR42_RS18265 0.67 -0.0 3,958,942 + RR42_RS18265 0.83 -0.6 3,958,942 + RR42_RS18265 0.83 +0.6 3,958,943 - RR42_RS18265 0.83 -1.5 3,959,053 + +1.5 3,959,053 + -0.2 3,959,054 - +0.4 3,959,103 + -0.7 3,959,511 + RR42_RS18270 0.24 +0.6 3,959,511 + RR42_RS18270 0.24 +2.4 3,959,511 + RR42_RS18270 0.24 -0.4 3,959,515 - RR42_RS18270 0.24 +1.4 3,959,876 + RR42_RS18270 0.55 +1.8 3,959,877 - RR42_RS18270 0.55 -0.7 3,959,933 + RR42_RS18270 0.60 -2.4 3,960,008 + RR42_RS18270 0.66 +1.6
Or see this region's nucleotide sequence