Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17290 and RR42_RS17295 are separated by 44 nucleotides RR42_RS17295 and RR42_RS17300 are separated by 253 nucleotides RR42_RS17300 and RR42_RS17305 are separated by 2 nucleotides
RR42_RS17290: RR42_RS17290 - LysR family transcriptional regulator, at 3,744,245 to 3,745,135
_RS17290
RR42_RS17295: RR42_RS17295 - acetyltransferase, at 3,745,180 to 3,745,707
_RS17295
RR42_RS17300: RR42_RS17300 - D-lactate dehydrogenase, FAD-linked subunit GlcD (EC 1.1.99.6) (from data) , at 3,745,961 to 3,747,454
_RS17300
RR42_RS17305: RR42_RS17305 - ion transporter, at 3,747,457 to 3,748,428
_RS17305
Position (kb)
3745
3746
3747
3748 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3745.053 kb on - strand at 3745.055 kb on + strand at 3745.055 kb on + strand at 3745.055 kb on + strand at 3745.055 kb on + strand at 3745.055 kb on + strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.100 kb on + strand at 3745.134 kb on - strand at 3745.134 kb on - strand at 3745.403 kb on + strand, within RR42_RS17295 at 3745.443 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.594 kb on + strand, within RR42_RS17295 at 3745.595 kb on - strand, within RR42_RS17295 at 3745.642 kb on + strand, within RR42_RS17295 at 3745.642 kb on + strand, within RR42_RS17295 at 3745.643 kb on - strand, within RR42_RS17295 at 3745.643 kb on - strand, within RR42_RS17295 at 3745.643 kb on - strand, within RR42_RS17295 at 3745.658 kb on - strand at 3745.705 kb on + strand at 3745.705 kb on + strand at 3745.705 kb on + strand at 3745.706 kb on - strand at 3745.742 kb on + strand at 3745.894 kb on + strand at 3745.894 kb on + strand at 3745.894 kb on + strand at 3745.894 kb on + strand at 3745.894 kb on + strand at 3745.895 kb on - strand at 3745.948 kb on - strand at 3746.085 kb on - strand at 3746.372 kb on + strand, within RR42_RS17300 at 3746.372 kb on + strand, within RR42_RS17300 at 3746.373 kb on - strand, within RR42_RS17300 at 3746.373 kb on - strand, within RR42_RS17300 at 3746.381 kb on + strand, within RR42_RS17300 at 3746.381 kb on + strand, within RR42_RS17300 at 3746.384 kb on + strand, within RR42_RS17300 at 3746.384 kb on + strand, within RR42_RS17300 at 3746.385 kb on - strand, within RR42_RS17300 at 3746.469 kb on - strand, within RR42_RS17300 at 3746.469 kb on - strand, within RR42_RS17300 at 3747.005 kb on + strand, within RR42_RS17300 at 3747.006 kb on - strand, within RR42_RS17300 at 3747.008 kb on + strand, within RR42_RS17300 at 3747.008 kb on + strand, within RR42_RS17300 at 3747.009 kb on - strand, within RR42_RS17300 at 3747.041 kb on - strand, within RR42_RS17300 at 3747.083 kb on + strand, within RR42_RS17300 at 3747.083 kb on + strand, within RR42_RS17300 at 3747.084 kb on - strand, within RR42_RS17300 at 3747.127 kb on + strand, within RR42_RS17300 at 3747.550 kb on + strand at 3747.550 kb on + strand at 3747.550 kb on + strand at 3747.550 kb on + strand at 3747.551 kb on - strand at 3747.663 kb on - strand, within RR42_RS17305 at 3747.720 kb on + strand, within RR42_RS17305 at 3747.913 kb on + strand, within RR42_RS17305 at 3747.913 kb on + strand, within RR42_RS17305 at 3747.913 kb on + strand, within RR42_RS17305 at 3748.024 kb on + strand, within RR42_RS17305 at 3748.024 kb on + strand, within RR42_RS17305 at 3748.024 kb on + strand, within RR42_RS17305 at 3748.024 kb on + strand, within RR42_RS17305 at 3748.024 kb on + strand, within RR42_RS17305 at 3748.024 kb on + strand, within RR42_RS17305 at 3748.024 kb on + strand, within RR42_RS17305 at 3748.024 kb on + strand, within RR42_RS17305 at 3748.024 kb on + strand, within RR42_RS17305 at 3748.025 kb on - strand, within RR42_RS17305 at 3748.076 kb on - strand, within RR42_RS17305 at 3748.175 kb on - strand, within RR42_RS17305 at 3748.301 kb on - strand, within RR42_RS17305 at 3748.333 kb on + strand at 3748.334 kb on - strand at 3748.431 kb on + strand at 3748.432 kb on - strand at 3748.432 kb on - strand at 3748.432 kb on - strand at 3748.432 kb on - strand at 3748.432 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,745,053 - -0.2 3,745,055 + -1.4 3,745,055 + +1.2 3,745,055 + -2.5 3,745,055 + -1.2 3,745,055 + -1.2 3,745,056 - +2.2 3,745,056 - +0.1 3,745,056 - +0.3 3,745,056 - +0.0 3,745,056 - -0.4 3,745,056 - +1.2 3,745,056 - -0.5 3,745,056 - -0.1 3,745,056 - +0.7 3,745,100 + -1.8 3,745,134 - +0.2 3,745,134 - -1.5 3,745,403 + RR42_RS17295 0.42 +1.9 3,745,443 + RR42_RS17295 0.50 +0.4 3,745,456 + RR42_RS17295 0.52 +0.5 3,745,456 + RR42_RS17295 0.52 +0.5 3,745,456 + RR42_RS17295 0.52 +0.3 3,745,456 + RR42_RS17295 0.52 -0.6 3,745,456 + RR42_RS17295 0.52 +0.1 3,745,456 + RR42_RS17295 0.52 +0.7 3,745,456 + RR42_RS17295 0.52 -0.5 3,745,456 + RR42_RS17295 0.52 -0.3 3,745,457 - RR42_RS17295 0.52 +0.7 3,745,457 - RR42_RS17295 0.52 -0.1 3,745,457 - RR42_RS17295 0.52 -0.3 3,745,457 - RR42_RS17295 0.52 +0.4 3,745,457 - RR42_RS17295 0.52 +0.4 3,745,457 - RR42_RS17295 0.52 -1.5 3,745,457 - RR42_RS17295 0.52 -0.6 3,745,594 + RR42_RS17295 0.78 +0.2 3,745,595 - RR42_RS17295 0.79 +0.7 3,745,642 + RR42_RS17295 0.88 +0.6 3,745,642 + RR42_RS17295 0.88 -1.1 3,745,643 - RR42_RS17295 0.88 -0.1 3,745,643 - RR42_RS17295 0.88 +0.7 3,745,643 - RR42_RS17295 0.88 -1.2 3,745,658 - -1.3 3,745,705 + -0.2 3,745,705 + +0.1 3,745,705 + +1.3 3,745,706 - +0.5 3,745,742 + +0.6 3,745,894 + -0.5 3,745,894 + -1.3 3,745,894 + -1.0 3,745,894 + +0.2 3,745,894 + -0.1 3,745,895 - -1.0 3,745,948 - +0.6 3,746,085 - -1.4 3,746,372 + RR42_RS17300 0.28 +0.1 3,746,372 + RR42_RS17300 0.28 +1.3 3,746,373 - RR42_RS17300 0.28 -1.7 3,746,373 - RR42_RS17300 0.28 -1.4 3,746,381 + RR42_RS17300 0.28 -0.4 3,746,381 + RR42_RS17300 0.28 -0.1 3,746,384 + RR42_RS17300 0.28 -0.3 3,746,384 + RR42_RS17300 0.28 +0.1 3,746,385 - RR42_RS17300 0.28 +0.6 3,746,469 - RR42_RS17300 0.34 +0.6 3,746,469 - RR42_RS17300 0.34 -1.2 3,747,005 + RR42_RS17300 0.70 +1.6 3,747,006 - RR42_RS17300 0.70 +0.6 3,747,008 + RR42_RS17300 0.70 +0.3 3,747,008 + RR42_RS17300 0.70 +0.2 3,747,009 - RR42_RS17300 0.70 +0.9 3,747,041 - RR42_RS17300 0.72 -0.4 3,747,083 + RR42_RS17300 0.75 +0.1 3,747,083 + RR42_RS17300 0.75 +2.2 3,747,084 - RR42_RS17300 0.75 +0.7 3,747,127 + RR42_RS17300 0.78 -2.4 3,747,550 + +0.8 3,747,550 + +0.6 3,747,550 + -1.6 3,747,550 + -0.3 3,747,551 - +0.2 3,747,663 - RR42_RS17305 0.21 +0.7 3,747,720 + RR42_RS17305 0.27 -0.0 3,747,913 + RR42_RS17305 0.47 +0.9 3,747,913 + RR42_RS17305 0.47 +0.2 3,747,913 + RR42_RS17305 0.47 +1.9 3,748,024 + RR42_RS17305 0.58 -0.1 3,748,024 + RR42_RS17305 0.58 -0.8 3,748,024 + RR42_RS17305 0.58 -1.2 3,748,024 + RR42_RS17305 0.58 -0.4 3,748,024 + RR42_RS17305 0.58 -0.1 3,748,024 + RR42_RS17305 0.58 +0.3 3,748,024 + RR42_RS17305 0.58 +0.9 3,748,024 + RR42_RS17305 0.58 +0.5 3,748,024 + RR42_RS17305 0.58 +0.6 3,748,025 - RR42_RS17305 0.58 +1.4 3,748,076 - RR42_RS17305 0.64 -2.8 3,748,175 - RR42_RS17305 0.74 -0.4 3,748,301 - RR42_RS17305 0.87 +0.3 3,748,333 + -0.5 3,748,334 - -2.8 3,748,431 + -0.2 3,748,432 - -1.0 3,748,432 - -1.4 3,748,432 - +1.2 3,748,432 - +2.2 3,748,432 - -0.1
Or see this region's nucleotide sequence