Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16540 and RR42_RS16545 are separated by 27 nucleotides RR42_RS16545 and RR42_RS16550 are separated by 283 nucleotides RR42_RS16550 and RR42_RS16555 are separated by 166 nucleotides RR42_RS16555 and RR42_RS16560 are separated by 211 nucleotides
RR42_RS16540: RR42_RS16540 - penicillin-binding protein, at 3,582,353 to 3,584,542
_RS16540
RR42_RS16545: RR42_RS16545 - prolyl-tRNA synthetase, at 3,584,570 to 3,585,034
_RS16545
RR42_RS16550: RR42_RS16550 - hydroperoxidase, at 3,585,318 to 3,587,519
_RS16550
RR42_RS16555: RR42_RS16555 - cysteine hydrolase, at 3,587,686 to 3,588,300
_RS16555
RR42_RS16560: RR42_RS16560 - acyl-CoA transferase, at 3,588,512 to 3,589,723
_RS16560
Position (kb)
3585
3586
3587
3588 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3584.371 kb on + strand at 3584.375 kb on + strand at 3584.424 kb on - strand at 3584.676 kb on + strand, within RR42_RS16545 at 3584.676 kb on + strand, within RR42_RS16545 at 3584.676 kb on + strand, within RR42_RS16545 at 3584.677 kb on - strand, within RR42_RS16545 at 3584.728 kb on - strand, within RR42_RS16545 at 3584.934 kb on + strand, within RR42_RS16545 at 3584.934 kb on + strand, within RR42_RS16545 at 3584.935 kb on - strand, within RR42_RS16545 at 3584.935 kb on - strand, within RR42_RS16545 at 3585.010 kb on + strand at 3585.071 kb on + strand at 3585.071 kb on + strand at 3585.072 kb on - strand at 3585.084 kb on + strand at 3585.085 kb on - strand at 3585.085 kb on - strand at 3585.086 kb on + strand at 3585.086 kb on + strand at 3585.087 kb on - strand at 3585.087 kb on - strand at 3585.119 kb on - strand at 3585.119 kb on - strand at 3585.157 kb on + strand at 3585.266 kb on + strand at 3585.267 kb on - strand at 3585.267 kb on - strand at 3585.269 kb on + strand at 3585.270 kb on - strand at 3585.282 kb on - strand at 3585.297 kb on + strand at 3585.400 kb on + strand at 3585.452 kb on + strand at 3585.452 kb on + strand at 3585.456 kb on + strand at 3585.456 kb on + strand at 3585.559 kb on - strand, within RR42_RS16550 at 3585.559 kb on - strand, within RR42_RS16550 at 3585.603 kb on + strand, within RR42_RS16550 at 3585.604 kb on - strand, within RR42_RS16550 at 3585.604 kb on - strand, within RR42_RS16550 at 3585.858 kb on + strand, within RR42_RS16550 at 3585.858 kb on + strand, within RR42_RS16550 at 3585.858 kb on + strand, within RR42_RS16550 at 3585.859 kb on - strand, within RR42_RS16550 at 3585.859 kb on - strand, within RR42_RS16550 at 3585.859 kb on - strand, within RR42_RS16550 at 3585.901 kb on - strand, within RR42_RS16550 at 3585.987 kb on + strand, within RR42_RS16550 at 3586.153 kb on + strand, within RR42_RS16550 at 3586.153 kb on + strand, within RR42_RS16550 at 3586.323 kb on + strand, within RR42_RS16550 at 3586.323 kb on + strand, within RR42_RS16550 at 3586.990 kb on + strand, within RR42_RS16550 at 3586.991 kb on - strand, within RR42_RS16550 at 3587.086 kb on - strand, within RR42_RS16550 at 3587.424 kb on + strand at 3587.424 kb on + strand at 3587.424 kb on + strand at 3587.425 kb on - strand at 3587.517 kb on + strand at 3587.518 kb on - strand at 3587.523 kb on + strand at 3587.523 kb on + strand at 3587.523 kb on + strand at 3587.523 kb on + strand at 3587.524 kb on - strand at 3587.524 kb on - strand at 3587.524 kb on - strand at 3587.524 kb on - strand at 3587.524 kb on - strand at 3587.631 kb on + strand at 3588.131 kb on + strand, within RR42_RS16555 at 3588.132 kb on - strand, within RR42_RS16555 at 3588.176 kb on + strand, within RR42_RS16555 at 3588.177 kb on - strand, within RR42_RS16555 at 3588.298 kb on + strand at 3588.299 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,584,371 + -2.2 3,584,375 + -0.1 3,584,424 - -1.0 3,584,676 + RR42_RS16545 0.23 +1.5 3,584,676 + RR42_RS16545 0.23 +1.1 3,584,676 + RR42_RS16545 0.23 +1.2 3,584,677 - RR42_RS16545 0.23 +0.3 3,584,728 - RR42_RS16545 0.34 +0.3 3,584,934 + RR42_RS16545 0.78 -0.2 3,584,934 + RR42_RS16545 0.78 +2.0 3,584,935 - RR42_RS16545 0.78 -1.1 3,584,935 - RR42_RS16545 0.78 +0.7 3,585,010 + +1.1 3,585,071 + -2.3 3,585,071 + +2.7 3,585,072 - +0.9 3,585,084 + -0.7 3,585,085 - -0.6 3,585,085 - -2.1 3,585,086 + -1.9 3,585,086 + -0.6 3,585,087 - +0.1 3,585,087 - -0.5 3,585,119 - -0.5 3,585,119 - +0.2 3,585,157 + -0.9 3,585,266 + -0.5 3,585,267 - +2.8 3,585,267 - +3.1 3,585,269 + +1.1 3,585,270 - -1.0 3,585,282 - +0.8 3,585,297 + +1.4 3,585,400 + +0.2 3,585,452 + +0.1 3,585,452 + -1.7 3,585,456 + -0.4 3,585,456 + +0.6 3,585,559 - RR42_RS16550 0.11 -0.3 3,585,559 - RR42_RS16550 0.11 -1.7 3,585,603 + RR42_RS16550 0.13 -0.7 3,585,604 - RR42_RS16550 0.13 +1.1 3,585,604 - RR42_RS16550 0.13 -0.8 3,585,858 + RR42_RS16550 0.25 -0.3 3,585,858 + RR42_RS16550 0.25 +0.9 3,585,858 + RR42_RS16550 0.25 +3.1 3,585,859 - RR42_RS16550 0.25 -0.2 3,585,859 - RR42_RS16550 0.25 +1.0 3,585,859 - RR42_RS16550 0.25 -0.7 3,585,901 - RR42_RS16550 0.26 +0.4 3,585,987 + RR42_RS16550 0.30 +0.9 3,586,153 + RR42_RS16550 0.38 -0.7 3,586,153 + RR42_RS16550 0.38 -0.7 3,586,323 + RR42_RS16550 0.46 +0.2 3,586,323 + RR42_RS16550 0.46 +0.3 3,586,990 + RR42_RS16550 0.76 -0.2 3,586,991 - RR42_RS16550 0.76 +1.1 3,587,086 - RR42_RS16550 0.80 +0.7 3,587,424 + -0.2 3,587,424 + +0.4 3,587,424 + +0.9 3,587,425 - -1.0 3,587,517 + -1.8 3,587,518 - -0.2 3,587,523 + -1.1 3,587,523 + -0.9 3,587,523 + -0.1 3,587,523 + -3.4 3,587,524 - +0.3 3,587,524 - -2.3 3,587,524 - +1.7 3,587,524 - +0.0 3,587,524 - -0.3 3,587,631 + -1.5 3,588,131 + RR42_RS16555 0.72 -1.0 3,588,132 - RR42_RS16555 0.73 -0.5 3,588,176 + RR42_RS16555 0.80 -0.9 3,588,177 - RR42_RS16555 0.80 -0.1 3,588,298 + -0.3 3,588,299 - +1.3
Or see this region's nucleotide sequence