Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16355 and RR42_RS16360 are separated by 445 nucleotides RR42_RS16360 and RR42_RS16365 are separated by 204 nucleotides
RR42_RS16355: RR42_RS16355 - hypothetical protein, at 3,542,559 to 3,543,650
_RS16355
RR42_RS16360: RR42_RS16360 - transposase, at 3,544,096 to 3,544,368
_RS16360
RR42_RS16365: RR42_RS16365 - 3-oxoacyl-ACP reductase, at 3,544,573 to 3,545,358
_RS16365
Position (kb)
3544
3545 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3543.116 kb on - strand, within RR42_RS16355 at 3543.156 kb on - strand, within RR42_RS16355 at 3543.157 kb on + strand, within RR42_RS16355 at 3543.298 kb on + strand, within RR42_RS16355 at 3543.298 kb on + strand, within RR42_RS16355 at 3543.408 kb on - strand, within RR42_RS16355 at 3543.408 kb on - strand, within RR42_RS16355 at 3543.538 kb on + strand, within RR42_RS16355 at 3543.674 kb on + strand at 3543.675 kb on - strand at 3543.965 kb on - strand at 3544.010 kb on - strand at 3544.013 kb on + strand at 3544.013 kb on + strand at 3544.013 kb on + strand at 3544.014 kb on - strand at 3544.104 kb on - strand at 3544.120 kb on + strand at 3544.123 kb on + strand at 3544.124 kb on - strand, within RR42_RS16360 at 3544.199 kb on - strand, within RR42_RS16360 at 3544.517 kb on + strand at 3544.518 kb on - strand at 3544.589 kb on + strand at 3544.590 kb on - strand at 3544.653 kb on + strand, within RR42_RS16365 at 3544.653 kb on + strand, within RR42_RS16365 at 3544.653 kb on + strand, within RR42_RS16365 at 3544.654 kb on - strand, within RR42_RS16365 at 3544.657 kb on + strand, within RR42_RS16365 at 3544.657 kb on + strand, within RR42_RS16365 at 3544.657 kb on + strand, within RR42_RS16365 at 3544.658 kb on - strand, within RR42_RS16365 at 3544.658 kb on - strand, within RR42_RS16365 at 3544.896 kb on + strand, within RR42_RS16365 at 3544.972 kb on - strand, within RR42_RS16365 at 3545.085 kb on + strand, within RR42_RS16365 at 3545.184 kb on + strand, within RR42_RS16365 at 3545.185 kb on - strand, within RR42_RS16365 at 3545.242 kb on + strand, within RR42_RS16365 at 3545.242 kb on + strand, within RR42_RS16365 at 3545.242 kb on + strand, within RR42_RS16365 at 3545.242 kb on + strand, within RR42_RS16365 at 3545.243 kb on - strand, within RR42_RS16365 at 3545.292 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,543,116 - RR42_RS16355 0.51 +0.0 3,543,156 - RR42_RS16355 0.55 -0.4 3,543,157 + RR42_RS16355 0.55 -0.0 3,543,298 + RR42_RS16355 0.68 +0.8 3,543,298 + RR42_RS16355 0.68 -4.7 3,543,408 - RR42_RS16355 0.78 +0.6 3,543,408 - RR42_RS16355 0.78 -1.1 3,543,538 + RR42_RS16355 0.90 -1.4 3,543,674 + -2.1 3,543,675 - -1.0 3,543,965 - -3.0 3,544,010 - +1.6 3,544,013 + -2.8 3,544,013 + +0.9 3,544,013 + -0.3 3,544,014 - -2.2 3,544,104 - -0.8 3,544,120 + +1.0 3,544,123 + -1.2 3,544,124 - RR42_RS16360 0.10 -1.4 3,544,199 - RR42_RS16360 0.38 -0.9 3,544,517 + -0.0 3,544,518 - -2.5 3,544,589 + -0.7 3,544,590 - +0.8 3,544,653 + RR42_RS16365 0.10 -0.6 3,544,653 + RR42_RS16365 0.10 +0.1 3,544,653 + RR42_RS16365 0.10 -0.2 3,544,654 - RR42_RS16365 0.10 +0.3 3,544,657 + RR42_RS16365 0.11 +1.4 3,544,657 + RR42_RS16365 0.11 +1.4 3,544,657 + RR42_RS16365 0.11 +0.6 3,544,658 - RR42_RS16365 0.11 +0.3 3,544,658 - RR42_RS16365 0.11 -0.4 3,544,896 + RR42_RS16365 0.41 +0.6 3,544,972 - RR42_RS16365 0.51 -0.8 3,545,085 + RR42_RS16365 0.65 +0.2 3,545,184 + RR42_RS16365 0.78 -2.1 3,545,185 - RR42_RS16365 0.78 -0.0 3,545,242 + RR42_RS16365 0.85 +0.1 3,545,242 + RR42_RS16365 0.85 -0.2 3,545,242 + RR42_RS16365 0.85 +0.8 3,545,242 + RR42_RS16365 0.85 -0.5 3,545,243 - RR42_RS16365 0.85 +0.0 3,545,292 + +0.2
Or see this region's nucleotide sequence