Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16210 and RR42_RS16215 are separated by 97 nucleotides RR42_RS16215 and RR42_RS16220 overlap by 4 nucleotides RR42_RS16220 and RR42_RS16225 are separated by 105 nucleotides
RR42_RS16210: RR42_RS16210 - dihydroxy-acid dehydratase, at 3,509,091 to 3,510,764
_RS16210
RR42_RS16215: RR42_RS16215 - hypothetical protein, at 3,510,862 to 3,511,455
_RS16215
RR42_RS16220: RR42_RS16220 - DNA polymerase III subunit chi, at 3,511,452 to 3,511,922
_RS16220
RR42_RS16225: RR42_RS16225 - cytosol aminopeptidase, at 3,512,028 to 3,513,596
_RS16225
Position (kb) 3511
3512 Strain fitness (log2 ratio) -2
-1
0
1 at 3511.137 kb on - strand, within RR42_RS16215 at 3511.849 kb on + strand, within RR42_RS16220 at 3511.953 kb on - strand at 3512.143 kb on + strand at 3512.143 kb on + strand at 3512.143 kb on + strand at 3512.143 kb on + strand at 3512.155 kb on + strand at 3512.248 kb on + strand, within RR42_RS16225 at 3512.249 kb on - strand, within RR42_RS16225 at 3512.431 kb on + strand, within RR42_RS16225 at 3512.431 kb on + strand, within RR42_RS16225 at 3512.432 kb on - strand, within RR42_RS16225 at 3512.432 kb on - strand, within RR42_RS16225 at 3512.432 kb on - strand, within RR42_RS16225 at 3512.432 kb on - strand, within RR42_RS16225 at 3512.650 kb on + strand, within RR42_RS16225 at 3512.650 kb on + strand, within RR42_RS16225 at 3512.651 kb on - strand, within RR42_RS16225 at 3512.651 kb on - strand, within RR42_RS16225 at 3512.762 kb on - strand, within RR42_RS16225 at 3512.912 kb on - strand, within RR42_RS16225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,511,137 - RR42_RS16215 0.46 -1.3 3,511,849 + RR42_RS16220 0.84 -1.0 3,511,953 - +0.8 3,512,143 + -0.0 3,512,143 + +0.4 3,512,143 + +1.6 3,512,143 + -0.5 3,512,155 + +0.8 3,512,248 + RR42_RS16225 0.14 +0.5 3,512,249 - RR42_RS16225 0.14 -2.0 3,512,431 + RR42_RS16225 0.26 +0.6 3,512,431 + RR42_RS16225 0.26 -0.8 3,512,432 - RR42_RS16225 0.26 +0.7 3,512,432 - RR42_RS16225 0.26 +1.0 3,512,432 - RR42_RS16225 0.26 +0.9 3,512,432 - RR42_RS16225 0.26 +0.6 3,512,650 + RR42_RS16225 0.40 +0.8 3,512,650 + RR42_RS16225 0.40 +0.7 3,512,651 - RR42_RS16225 0.40 +1.0 3,512,651 - RR42_RS16225 0.40 +0.9 3,512,762 - RR42_RS16225 0.47 +1.1 3,512,912 - RR42_RS16225 0.56 +0.1
Or see this region's nucleotide sequence