Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15520 and RR42_RS15525 are separated by 186 nucleotides RR42_RS15525 and RR42_RS15530 overlap by 4 nucleotides RR42_RS15530 and RR42_RS15535 are separated by 74 nucleotides RR42_RS15535 and RR42_RS15540 are separated by 95 nucleotides
RR42_RS15520: RR42_RS15520 - hypothetical protein, at 3,354,208 to 3,354,639
_RS15520
RR42_RS15525: RR42_RS15525 - hypothetical protein, at 3,354,826 to 3,355,266
_RS15525
RR42_RS15530: RR42_RS15530 - membrane protein, at 3,355,263 to 3,356,195
_RS15530
RR42_RS15535: RR42_RS15535 - hypothetical protein, at 3,356,270 to 3,356,818
_RS15535
RR42_RS15540: RR42_RS15540 - hypothetical protein, at 3,356,914 to 3,358,347
_RS15540
Position (kb)
3355
3356
3357 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3354.325 kb on + strand, within RR42_RS15520 at 3354.325 kb on + strand, within RR42_RS15520 at 3354.326 kb on - strand, within RR42_RS15520 at 3354.326 kb on - strand, within RR42_RS15520 at 3354.397 kb on + strand, within RR42_RS15520 at 3354.415 kb on + strand, within RR42_RS15520 at 3354.415 kb on + strand, within RR42_RS15520 at 3354.481 kb on + strand, within RR42_RS15520 at 3354.482 kb on - strand, within RR42_RS15520 at 3354.526 kb on + strand, within RR42_RS15520 at 3354.526 kb on + strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.715 kb on + strand at 3354.716 kb on - strand at 3354.756 kb on - strand at 3354.790 kb on + strand at 3354.998 kb on - strand, within RR42_RS15525 at 3355.378 kb on - strand, within RR42_RS15530 at 3355.458 kb on - strand, within RR42_RS15530 at 3355.556 kb on + strand, within RR42_RS15530 at 3355.557 kb on - strand, within RR42_RS15530 at 3355.726 kb on - strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.748 kb on + strand, within RR42_RS15530 at 3355.749 kb on - strand, within RR42_RS15530 at 3355.791 kb on + strand, within RR42_RS15530 at 3355.792 kb on - strand, within RR42_RS15530 at 3355.970 kb on - strand, within RR42_RS15530 at 3355.970 kb on - strand, within RR42_RS15530 at 3356.042 kb on + strand, within RR42_RS15530 at 3356.043 kb on - strand, within RR42_RS15530 at 3356.043 kb on - strand, within RR42_RS15530 at 3356.165 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.189 kb on - strand at 3356.189 kb on - strand at 3356.189 kb on - strand at 3356.342 kb on + strand, within RR42_RS15535 at 3356.342 kb on + strand, within RR42_RS15535 at 3356.443 kb on - strand, within RR42_RS15535 at 3356.446 kb on + strand, within RR42_RS15535 at 3356.661 kb on - strand, within RR42_RS15535 at 3356.715 kb on - strand, within RR42_RS15535 at 3356.715 kb on - strand, within RR42_RS15535 at 3356.796 kb on + strand at 3356.796 kb on + strand at 3356.797 kb on - strand at 3356.797 kb on - strand at 3357.057 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,354,325 + RR42_RS15520 0.27 +0.2 3,354,325 + RR42_RS15520 0.27 +0.2 3,354,326 - RR42_RS15520 0.27 +0.1 3,354,326 - RR42_RS15520 0.27 -0.5 3,354,397 + RR42_RS15520 0.44 -0.6 3,354,415 + RR42_RS15520 0.48 -0.7 3,354,415 + RR42_RS15520 0.48 +0.8 3,354,481 + RR42_RS15520 0.63 -0.1 3,354,482 - RR42_RS15520 0.63 +1.0 3,354,526 + RR42_RS15520 0.74 -1.0 3,354,526 + RR42_RS15520 0.74 +0.8 3,354,527 - RR42_RS15520 0.74 +0.7 3,354,527 - RR42_RS15520 0.74 +0.5 3,354,527 - RR42_RS15520 0.74 -0.1 3,354,715 + -0.5 3,354,716 - -2.5 3,354,756 - +1.5 3,354,790 + -0.4 3,354,998 - RR42_RS15525 0.39 -1.4 3,355,378 - RR42_RS15530 0.12 +0.4 3,355,458 - RR42_RS15530 0.21 -1.3 3,355,556 + RR42_RS15530 0.31 +0.7 3,355,557 - RR42_RS15530 0.32 -0.5 3,355,726 - RR42_RS15530 0.50 -1.1 3,355,746 + RR42_RS15530 0.52 -1.0 3,355,746 + RR42_RS15530 0.52 -0.5 3,355,746 + RR42_RS15530 0.52 -0.4 3,355,746 + RR42_RS15530 0.52 -1.7 3,355,746 + RR42_RS15530 0.52 +0.1 3,355,746 + RR42_RS15530 0.52 +0.6 3,355,746 + RR42_RS15530 0.52 -1.0 3,355,746 + RR42_RS15530 0.52 +1.7 3,355,746 + RR42_RS15530 0.52 -0.2 3,355,746 + RR42_RS15530 0.52 -1.7 3,355,747 - RR42_RS15530 0.52 -0.2 3,355,747 - RR42_RS15530 0.52 +0.1 3,355,747 - RR42_RS15530 0.52 -0.4 3,355,747 - RR42_RS15530 0.52 -2.0 3,355,747 - RR42_RS15530 0.52 +0.2 3,355,748 + RR42_RS15530 0.52 -1.2 3,355,749 - RR42_RS15530 0.52 -1.2 3,355,791 + RR42_RS15530 0.57 -0.1 3,355,792 - RR42_RS15530 0.57 +0.5 3,355,970 - RR42_RS15530 0.76 -3.1 3,355,970 - RR42_RS15530 0.76 +0.8 3,356,042 + RR42_RS15530 0.83 +1.6 3,356,043 - RR42_RS15530 0.84 -1.5 3,356,043 - RR42_RS15530 0.84 -0.3 3,356,165 + -4.2 3,356,188 + +0.5 3,356,188 + -2.0 3,356,188 + +0.0 3,356,188 + -1.2 3,356,188 + +1.0 3,356,188 + +0.6 3,356,188 + -0.5 3,356,188 + -0.1 3,356,189 - +0.6 3,356,189 - -0.8 3,356,189 - -2.0 3,356,342 + RR42_RS15535 0.13 -1.7 3,356,342 + RR42_RS15535 0.13 -0.3 3,356,443 - RR42_RS15535 0.32 -1.1 3,356,446 + RR42_RS15535 0.32 +0.5 3,356,661 - RR42_RS15535 0.71 -2.5 3,356,715 - RR42_RS15535 0.81 +0.2 3,356,715 - RR42_RS15535 0.81 +0.7 3,356,796 + +0.4 3,356,796 + -0.9 3,356,797 - -0.7 3,356,797 - -0.3 3,357,057 - +0.1
Or see this region's nucleotide sequence