Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15425 and RR42_RS15430 are separated by 45 nucleotides RR42_RS15430 and RR42_RS15435 are separated by 88 nucleotides RR42_RS15435 and RR42_RS15440 are separated by 71 nucleotides
RR42_RS15425: RR42_RS15425 - membrane protein, at 3,333,362 to 3,334,519
_RS15425
RR42_RS15430: RR42_RS15430 - peptidase, at 3,334,565 to 3,336,460
_RS15430
RR42_RS15435: RR42_RS15435 - tRNA-Lys, at 3,336,549 to 3,336,624
_RS15435
RR42_RS15440: RR42_RS15440 - transporter, at 3,336,696 to 3,337,811
_RS15440
Position (kb)
3334
3335
3336
3337 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3333.620 kb on + strand, within RR42_RS15425 at 3333.621 kb on - strand, within RR42_RS15425 at 3333.659 kb on + strand, within RR42_RS15425 at 3334.142 kb on + strand, within RR42_RS15425 at 3334.143 kb on - strand, within RR42_RS15425 at 3334.143 kb on - strand, within RR42_RS15425 at 3334.355 kb on - strand, within RR42_RS15425 at 3334.619 kb on + strand at 3334.620 kb on - strand at 3334.841 kb on + strand, within RR42_RS15430 at 3334.978 kb on + strand, within RR42_RS15430 at 3335.013 kb on + strand, within RR42_RS15430 at 3335.013 kb on + strand, within RR42_RS15430 at 3335.013 kb on + strand, within RR42_RS15430 at 3335.236 kb on + strand, within RR42_RS15430 at 3335.237 kb on - strand, within RR42_RS15430 at 3335.258 kb on + strand, within RR42_RS15430 at 3335.258 kb on + strand, within RR42_RS15430 at 3335.259 kb on - strand, within RR42_RS15430 at 3335.259 kb on - strand, within RR42_RS15430 at 3335.259 kb on - strand, within RR42_RS15430 at 3335.295 kb on + strand, within RR42_RS15430 at 3335.355 kb on + strand, within RR42_RS15430 at 3335.355 kb on + strand, within RR42_RS15430 at 3335.355 kb on + strand, within RR42_RS15430 at 3335.355 kb on + strand, within RR42_RS15430 at 3335.355 kb on + strand, within RR42_RS15430 at 3335.356 kb on - strand, within RR42_RS15430 at 3335.356 kb on - strand, within RR42_RS15430 at 3335.356 kb on - strand, within RR42_RS15430 at 3335.356 kb on - strand, within RR42_RS15430 at 3335.356 kb on - strand, within RR42_RS15430 at 3335.356 kb on - strand, within RR42_RS15430 at 3335.376 kb on - strand, within RR42_RS15430 at 3335.376 kb on - strand at 3335.411 kb on + strand, within RR42_RS15430 at 3335.411 kb on + strand, within RR42_RS15430 at 3335.411 kb on + strand, within RR42_RS15430 at 3335.412 kb on - strand, within RR42_RS15430 at 3335.803 kb on - strand, within RR42_RS15430 at 3335.803 kb on - strand, within RR42_RS15430 at 3335.803 kb on - strand, within RR42_RS15430 at 3335.803 kb on - strand, within RR42_RS15430 at 3336.008 kb on + strand, within RR42_RS15430 at 3336.020 kb on + strand, within RR42_RS15430 at 3336.086 kb on + strand, within RR42_RS15430 at 3336.350 kb on + strand at 3336.351 kb on - strand at 3336.411 kb on - strand at 3336.517 kb on + strand at 3336.518 kb on - strand at 3336.518 kb on - strand at 3336.518 kb on - strand at 3336.689 kb on - strand at 3336.698 kb on - strand at 3336.799 kb on - strand at 3336.940 kb on + strand, within RR42_RS15440 at 3337.036 kb on + strand, within RR42_RS15440 at 3337.037 kb on - strand, within RR42_RS15440 at 3337.037 kb on - strand, within RR42_RS15440 at 3337.037 kb on - strand, within RR42_RS15440 at 3337.037 kb on - strand, within RR42_RS15440 at 3337.043 kb on - strand, within RR42_RS15440 at 3337.150 kb on + strand, within RR42_RS15440 at 3337.150 kb on + strand, within RR42_RS15440 at 3337.151 kb on - strand, within RR42_RS15440 at 3337.151 kb on - strand, within RR42_RS15440 at 3337.407 kb on - strand, within RR42_RS15440 at 3337.409 kb on - strand, within RR42_RS15440 at 3337.409 kb on - strand, within RR42_RS15440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,333,620 + RR42_RS15425 0.22 -1.1 3,333,621 - RR42_RS15425 0.22 +1.0 3,333,659 + RR42_RS15425 0.26 -1.1 3,334,142 + RR42_RS15425 0.67 -0.5 3,334,143 - RR42_RS15425 0.67 -0.5 3,334,143 - RR42_RS15425 0.67 -0.5 3,334,355 - RR42_RS15425 0.86 -1.3 3,334,619 + -0.6 3,334,620 - +0.6 3,334,841 + RR42_RS15430 0.15 +1.0 3,334,978 + RR42_RS15430 0.22 +0.0 3,335,013 + RR42_RS15430 0.24 -0.2 3,335,013 + RR42_RS15430 0.24 -2.3 3,335,013 + RR42_RS15430 0.24 +1.6 3,335,236 + RR42_RS15430 0.35 -0.2 3,335,237 - RR42_RS15430 0.35 +0.1 3,335,258 + RR42_RS15430 0.37 +0.3 3,335,258 + RR42_RS15430 0.37 -3.1 3,335,259 - RR42_RS15430 0.37 -0.6 3,335,259 - RR42_RS15430 0.37 +0.8 3,335,259 - RR42_RS15430 0.37 +0.7 3,335,295 + RR42_RS15430 0.39 +0.5 3,335,355 + RR42_RS15430 0.42 -4.3 3,335,355 + RR42_RS15430 0.42 +0.8 3,335,355 + RR42_RS15430 0.42 +0.8 3,335,355 + RR42_RS15430 0.42 -0.7 3,335,355 + RR42_RS15430 0.42 -0.1 3,335,356 - RR42_RS15430 0.42 +0.7 3,335,356 - RR42_RS15430 0.42 +1.0 3,335,356 - RR42_RS15430 0.42 -0.2 3,335,356 - RR42_RS15430 0.42 +0.6 3,335,356 - RR42_RS15430 0.42 -0.6 3,335,356 - RR42_RS15430 0.42 -0.2 3,335,376 - RR42_RS15430 0.43 -1.2 3,335,376 - +0.0 3,335,411 + RR42_RS15430 0.45 +0.4 3,335,411 + RR42_RS15430 0.45 -0.5 3,335,411 + RR42_RS15430 0.45 -0.3 3,335,412 - RR42_RS15430 0.45 +0.4 3,335,803 - RR42_RS15430 0.65 -1.7 3,335,803 - RR42_RS15430 0.65 -1.6 3,335,803 - RR42_RS15430 0.65 -0.8 3,335,803 - RR42_RS15430 0.65 -1.1 3,336,008 + RR42_RS15430 0.76 -1.5 3,336,020 + RR42_RS15430 0.77 -0.4 3,336,086 + RR42_RS15430 0.80 +1.0 3,336,350 + -2.0 3,336,351 - -0.3 3,336,411 - -2.6 3,336,517 + +0.8 3,336,518 - +0.8 3,336,518 - -2.3 3,336,518 - -1.2 3,336,689 - -0.3 3,336,698 - +1.4 3,336,799 - -0.2 3,336,940 + RR42_RS15440 0.22 -2.0 3,337,036 + RR42_RS15440 0.30 +2.4 3,337,037 - RR42_RS15440 0.31 -2.0 3,337,037 - RR42_RS15440 0.31 -0.7 3,337,037 - RR42_RS15440 0.31 -1.5 3,337,037 - RR42_RS15440 0.31 -2.0 3,337,043 - RR42_RS15440 0.31 +2.0 3,337,150 + RR42_RS15440 0.41 +1.5 3,337,150 + RR42_RS15440 0.41 +0.2 3,337,151 - RR42_RS15440 0.41 +0.1 3,337,151 - RR42_RS15440 0.41 +1.0 3,337,407 - RR42_RS15440 0.64 +0.2 3,337,409 - RR42_RS15440 0.64 +0.4 3,337,409 - RR42_RS15440 0.64 +0.4
Or see this region's nucleotide sequence