Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15330 and RR42_RS15335 are separated by 16 nucleotides RR42_RS15335 and RR42_RS15340 are separated by 50 nucleotides RR42_RS15340 and RR42_RS15345 are separated by 92 nucleotides RR42_RS15345 and RR42_RS15350 overlap by 4 nucleotides
RR42_RS15330: RR42_RS15330 - hypothetical protein, at 3,316,896 to 3,317,981
_RS15330
RR42_RS15335: RR42_RS15335 - peptide methionine sulfoxide reductase, at 3,317,998 to 3,318,540
_RS15335
RR42_RS15340: RR42_RS15340 - hypothetical protein, at 3,318,591 to 3,318,866
_RS15340
RR42_RS15345: RR42_RS15345 - long-chain fatty acid--CoA ligase, at 3,318,959 to 3,320,512
_RS15345
RR42_RS15350: RR42_RS15350 - BEC protein, at 3,320,509 to 3,321,753
_RS15350
Position (kb)
3318
3319
3320
3321 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3318.011 kb on + strand at 3318.011 kb on + strand at 3318.012 kb on - strand at 3318.068 kb on + strand, within RR42_RS15335 at 3318.095 kb on + strand, within RR42_RS15335 at 3318.095 kb on + strand, within RR42_RS15335 at 3318.096 kb on - strand, within RR42_RS15335 at 3318.125 kb on + strand, within RR42_RS15335 at 3318.126 kb on - strand, within RR42_RS15335 at 3318.200 kb on + strand, within RR42_RS15335 at 3318.200 kb on + strand, within RR42_RS15335 at 3318.421 kb on + strand, within RR42_RS15335 at 3318.421 kb on + strand, within RR42_RS15335 at 3318.421 kb on + strand, within RR42_RS15335 at 3318.422 kb on - strand, within RR42_RS15335 at 3318.422 kb on - strand, within RR42_RS15335 at 3318.435 kb on + strand, within RR42_RS15335 at 3318.437 kb on + strand, within RR42_RS15335 at 3318.565 kb on + strand at 3318.565 kb on + strand at 3318.565 kb on + strand at 3318.565 kb on + strand at 3318.566 kb on - strand at 3318.569 kb on + strand at 3318.569 kb on + strand at 3318.569 kb on + strand at 3318.570 kb on - strand at 3318.570 kb on - strand at 3318.570 kb on - strand at 3318.570 kb on - strand at 3318.575 kb on - strand at 3318.575 kb on - strand at 3318.575 kb on - strand at 3318.702 kb on - strand, within RR42_RS15340 at 3318.716 kb on - strand, within RR42_RS15340 at 3318.716 kb on - strand, within RR42_RS15340 at 3318.788 kb on - strand, within RR42_RS15340 at 3318.788 kb on - strand, within RR42_RS15340 at 3318.791 kb on + strand, within RR42_RS15340 at 3318.792 kb on - strand, within RR42_RS15340 at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.869 kb on - strand at 3318.869 kb on - strand at 3318.869 kb on - strand at 3318.870 kb on + strand at 3318.870 kb on + strand at 3318.870 kb on + strand at 3318.870 kb on + strand at 3318.871 kb on - strand at 3318.871 kb on - strand at 3318.871 kb on - strand at 3318.878 kb on - strand at 3318.941 kb on + strand at 3318.941 kb on + strand at 3318.942 kb on - strand at 3319.252 kb on - strand, within RR42_RS15345 at 3319.389 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.992 kb on + strand, within RR42_RS15345 at 3319.992 kb on + strand, within RR42_RS15345 at 3319.992 kb on + strand, within RR42_RS15345 at 3319.992 kb on + strand, within RR42_RS15345 at 3319.993 kb on - strand, within RR42_RS15345 at 3319.993 kb on - strand, within RR42_RS15345 at 3319.993 kb on - strand, within RR42_RS15345 at 3319.993 kb on - strand, within RR42_RS15345 at 3320.382 kb on + strand at 3320.382 kb on + strand at 3320.383 kb on - strand at 3320.392 kb on + strand at 3320.392 kb on + strand at 3320.393 kb on - strand at 3320.404 kb on + strand at 3320.404 kb on + strand at 3320.404 kb on + strand at 3320.641 kb on + strand, within RR42_RS15350 at 3320.711 kb on + strand, within RR42_RS15350 at 3320.711 kb on + strand, within RR42_RS15350 at 3320.711 kb on - strand, within RR42_RS15350 at 3320.847 kb on - strand, within RR42_RS15350 at 3320.912 kb on + strand, within RR42_RS15350 at 3320.912 kb on + strand, within RR42_RS15350 at 3320.937 kb on - strand, within RR42_RS15350 at 3320.987 kb on + strand, within RR42_RS15350 at 3320.987 kb on + strand, within RR42_RS15350 at 3320.988 kb on - strand, within RR42_RS15350 at 3320.988 kb on - strand, within RR42_RS15350 at 3320.991 kb on - strand, within RR42_RS15350 at 3320.991 kb on - strand, within RR42_RS15350 at 3321.134 kb on + strand, within RR42_RS15350 at 3321.134 kb on + strand, within RR42_RS15350 at 3321.134 kb on + strand, within RR42_RS15350 at 3321.135 kb on - strand, within RR42_RS15350 at 3321.194 kb on + strand, within RR42_RS15350 at 3321.195 kb on - strand, within RR42_RS15350 at 3321.198 kb on - strand, within RR42_RS15350 at 3321.332 kb on + strand, within RR42_RS15350 at 3321.332 kb on + strand, within RR42_RS15350 at 3321.333 kb on - strand, within RR42_RS15350 at 3321.333 kb on - strand, within RR42_RS15350 at 3321.333 kb on - strand, within RR42_RS15350
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,318,011 + -0.6 3,318,011 + -0.9 3,318,012 - -0.1 3,318,068 + RR42_RS15335 0.13 +0.2 3,318,095 + RR42_RS15335 0.18 +1.0 3,318,095 + RR42_RS15335 0.18 +0.0 3,318,096 - RR42_RS15335 0.18 +1.0 3,318,125 + RR42_RS15335 0.23 +0.8 3,318,126 - RR42_RS15335 0.24 +0.5 3,318,200 + RR42_RS15335 0.37 +0.0 3,318,200 + RR42_RS15335 0.37 -0.2 3,318,421 + RR42_RS15335 0.78 +1.0 3,318,421 + RR42_RS15335 0.78 +1.0 3,318,421 + RR42_RS15335 0.78 +0.8 3,318,422 - RR42_RS15335 0.78 +0.4 3,318,422 - RR42_RS15335 0.78 +0.8 3,318,435 + RR42_RS15335 0.80 -3.3 3,318,437 + RR42_RS15335 0.81 -0.6 3,318,565 + -0.3 3,318,565 + +1.0 3,318,565 + +1.0 3,318,565 + -0.1 3,318,566 - +4.0 3,318,569 + +0.9 3,318,569 + -2.0 3,318,569 + -1.5 3,318,570 - -2.3 3,318,570 - -2.5 3,318,570 - +1.3 3,318,570 - +1.0 3,318,575 - -2.1 3,318,575 - -2.9 3,318,575 - -0.3 3,318,702 - RR42_RS15340 0.40 -0.9 3,318,716 - RR42_RS15340 0.45 +0.4 3,318,716 - RR42_RS15340 0.45 -1.0 3,318,788 - RR42_RS15340 0.71 -2.3 3,318,788 - RR42_RS15340 0.71 +0.2 3,318,791 + RR42_RS15340 0.72 -0.5 3,318,792 - RR42_RS15340 0.73 +0.0 3,318,868 + +0.0 3,318,868 + -2.1 3,318,868 + +0.4 3,318,868 + +0.2 3,318,868 + -0.2 3,318,868 + -0.0 3,318,869 - -3.2 3,318,869 - -0.9 3,318,869 - -1.0 3,318,870 + +0.0 3,318,870 + +0.4 3,318,870 + -0.2 3,318,870 + +0.2 3,318,871 - +0.2 3,318,871 - +0.5 3,318,871 - +1.0 3,318,878 - -0.2 3,318,941 + -0.4 3,318,941 + -1.5 3,318,942 - +0.4 3,319,252 - RR42_RS15345 0.19 -2.3 3,319,389 + RR42_RS15345 0.28 -0.6 3,319,620 + RR42_RS15345 0.43 +0.7 3,319,620 + RR42_RS15345 0.43 -0.4 3,319,620 + RR42_RS15345 0.43 -2.3 3,319,620 + RR42_RS15345 0.43 +0.9 3,319,621 - RR42_RS15345 0.43 +0.2 3,319,621 - RR42_RS15345 0.43 +1.6 3,319,621 - RR42_RS15345 0.43 -0.3 3,319,621 - RR42_RS15345 0.43 -0.3 3,319,621 - RR42_RS15345 0.43 +0.8 3,319,992 + RR42_RS15345 0.66 -0.1 3,319,992 + RR42_RS15345 0.66 -0.7 3,319,992 + RR42_RS15345 0.66 +3.0 3,319,992 + RR42_RS15345 0.66 +1.0 3,319,993 - RR42_RS15345 0.67 -0.7 3,319,993 - RR42_RS15345 0.67 +0.4 3,319,993 - RR42_RS15345 0.67 +1.3 3,319,993 - RR42_RS15345 0.67 -1.0 3,320,382 + -0.5 3,320,382 + +0.3 3,320,383 - -0.0 3,320,392 + -0.7 3,320,392 + +4.0 3,320,393 - -1.5 3,320,404 + -1.6 3,320,404 + +1.2 3,320,404 + -1.5 3,320,641 + RR42_RS15350 0.11 -0.9 3,320,711 + RR42_RS15350 0.16 +0.7 3,320,711 + RR42_RS15350 0.16 -1.8 3,320,711 - RR42_RS15350 0.16 +0.7 3,320,847 - RR42_RS15350 0.27 +1.1 3,320,912 + RR42_RS15350 0.32 -0.6 3,320,912 + RR42_RS15350 0.32 +0.9 3,320,937 - RR42_RS15350 0.34 -0.0 3,320,987 + RR42_RS15350 0.38 -0.1 3,320,987 + RR42_RS15350 0.38 +1.5 3,320,988 - RR42_RS15350 0.38 -1.5 3,320,988 - RR42_RS15350 0.38 +1.1 3,320,991 - RR42_RS15350 0.39 -1.1 3,320,991 - RR42_RS15350 0.39 -0.1 3,321,134 + RR42_RS15350 0.50 -0.8 3,321,134 + RR42_RS15350 0.50 +0.8 3,321,134 + RR42_RS15350 0.50 +1.1 3,321,135 - RR42_RS15350 0.50 -0.8 3,321,194 + RR42_RS15350 0.55 -0.1 3,321,195 - RR42_RS15350 0.55 -0.7 3,321,198 - RR42_RS15350 0.55 -0.0 3,321,332 + RR42_RS15350 0.66 -0.2 3,321,332 + RR42_RS15350 0.66 -0.4 3,321,333 - RR42_RS15350 0.66 +1.3 3,321,333 - RR42_RS15350 0.66 +0.9 3,321,333 - RR42_RS15350 0.66 +0.6
Or see this region's nucleotide sequence