Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15330 and RR42_RS15335 are separated by 16 nucleotides RR42_RS15335 and RR42_RS15340 are separated by 50 nucleotides RR42_RS15340 and RR42_RS15345 are separated by 92 nucleotides
RR42_RS15330: RR42_RS15330 - hypothetical protein, at 3,316,896 to 3,317,981
_RS15330
RR42_RS15335: RR42_RS15335 - peptide methionine sulfoxide reductase, at 3,317,998 to 3,318,540
_RS15335
RR42_RS15340: RR42_RS15340 - hypothetical protein, at 3,318,591 to 3,318,866
_RS15340
RR42_RS15345: RR42_RS15345 - long-chain fatty acid--CoA ligase, at 3,318,959 to 3,320,512
_RS15345
Position (kb)
3318
3319 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3317.618 kb on + strand, within RR42_RS15330 at 3317.618 kb on + strand, within RR42_RS15330 at 3317.619 kb on - strand, within RR42_RS15330 at 3317.772 kb on + strand, within RR42_RS15330 at 3317.793 kb on + strand, within RR42_RS15330 at 3317.889 kb on + strand at 3317.889 kb on + strand at 3317.889 kb on + strand at 3317.890 kb on - strand at 3318.011 kb on + strand at 3318.011 kb on + strand at 3318.012 kb on - strand at 3318.068 kb on + strand, within RR42_RS15335 at 3318.095 kb on + strand, within RR42_RS15335 at 3318.095 kb on + strand, within RR42_RS15335 at 3318.096 kb on - strand, within RR42_RS15335 at 3318.125 kb on + strand, within RR42_RS15335 at 3318.126 kb on - strand, within RR42_RS15335 at 3318.200 kb on + strand, within RR42_RS15335 at 3318.200 kb on + strand, within RR42_RS15335 at 3318.421 kb on + strand, within RR42_RS15335 at 3318.421 kb on + strand, within RR42_RS15335 at 3318.421 kb on + strand, within RR42_RS15335 at 3318.422 kb on - strand, within RR42_RS15335 at 3318.422 kb on - strand, within RR42_RS15335 at 3318.435 kb on + strand, within RR42_RS15335 at 3318.437 kb on + strand, within RR42_RS15335 at 3318.565 kb on + strand at 3318.565 kb on + strand at 3318.565 kb on + strand at 3318.565 kb on + strand at 3318.566 kb on - strand at 3318.569 kb on + strand at 3318.569 kb on + strand at 3318.569 kb on + strand at 3318.570 kb on - strand at 3318.570 kb on - strand at 3318.570 kb on - strand at 3318.570 kb on - strand at 3318.575 kb on - strand at 3318.575 kb on - strand at 3318.575 kb on - strand at 3318.702 kb on - strand, within RR42_RS15340 at 3318.716 kb on - strand, within RR42_RS15340 at 3318.716 kb on - strand, within RR42_RS15340 at 3318.788 kb on - strand, within RR42_RS15340 at 3318.788 kb on - strand, within RR42_RS15340 at 3318.791 kb on + strand, within RR42_RS15340 at 3318.792 kb on - strand, within RR42_RS15340 at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.869 kb on - strand at 3318.869 kb on - strand at 3318.869 kb on - strand at 3318.870 kb on + strand at 3318.870 kb on + strand at 3318.870 kb on + strand at 3318.870 kb on + strand at 3318.871 kb on - strand at 3318.871 kb on - strand at 3318.871 kb on - strand at 3318.878 kb on - strand at 3318.941 kb on + strand at 3318.941 kb on + strand at 3318.942 kb on - strand at 3319.252 kb on - strand, within RR42_RS15345 at 3319.389 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,317,618 + RR42_RS15330 0.66 +0.2 3,317,618 + RR42_RS15330 0.66 -0.3 3,317,619 - RR42_RS15330 0.67 +0.0 3,317,772 + RR42_RS15330 0.81 -1.7 3,317,793 + RR42_RS15330 0.83 +1.0 3,317,889 + -3.1 3,317,889 + -0.5 3,317,889 + +0.4 3,317,890 - +0.7 3,318,011 + -0.6 3,318,011 + -0.9 3,318,012 - -0.1 3,318,068 + RR42_RS15335 0.13 +0.2 3,318,095 + RR42_RS15335 0.18 +1.0 3,318,095 + RR42_RS15335 0.18 +0.0 3,318,096 - RR42_RS15335 0.18 +1.0 3,318,125 + RR42_RS15335 0.23 +0.8 3,318,126 - RR42_RS15335 0.24 +0.5 3,318,200 + RR42_RS15335 0.37 +0.0 3,318,200 + RR42_RS15335 0.37 -0.2 3,318,421 + RR42_RS15335 0.78 +1.0 3,318,421 + RR42_RS15335 0.78 +1.0 3,318,421 + RR42_RS15335 0.78 +0.8 3,318,422 - RR42_RS15335 0.78 +0.4 3,318,422 - RR42_RS15335 0.78 +0.8 3,318,435 + RR42_RS15335 0.80 -3.3 3,318,437 + RR42_RS15335 0.81 -0.6 3,318,565 + -0.3 3,318,565 + +1.0 3,318,565 + +1.0 3,318,565 + -0.1 3,318,566 - +4.0 3,318,569 + +0.9 3,318,569 + -2.0 3,318,569 + -1.5 3,318,570 - -2.3 3,318,570 - -2.5 3,318,570 - +1.3 3,318,570 - +1.0 3,318,575 - -2.1 3,318,575 - -2.9 3,318,575 - -0.3 3,318,702 - RR42_RS15340 0.40 -0.9 3,318,716 - RR42_RS15340 0.45 +0.4 3,318,716 - RR42_RS15340 0.45 -1.0 3,318,788 - RR42_RS15340 0.71 -2.3 3,318,788 - RR42_RS15340 0.71 +0.2 3,318,791 + RR42_RS15340 0.72 -0.5 3,318,792 - RR42_RS15340 0.73 +0.0 3,318,868 + +0.0 3,318,868 + -2.1 3,318,868 + +0.4 3,318,868 + +0.2 3,318,868 + -0.2 3,318,868 + -0.0 3,318,869 - -3.2 3,318,869 - -0.9 3,318,869 - -1.0 3,318,870 + +0.0 3,318,870 + +0.4 3,318,870 + -0.2 3,318,870 + +0.2 3,318,871 - +0.2 3,318,871 - +0.5 3,318,871 - +1.0 3,318,878 - -0.2 3,318,941 + -0.4 3,318,941 + -1.5 3,318,942 - +0.4 3,319,252 - RR42_RS15345 0.19 -2.3 3,319,389 + RR42_RS15345 0.28 -0.6 3,319,620 + RR42_RS15345 0.43 +0.7 3,319,620 + RR42_RS15345 0.43 -0.4 3,319,620 + RR42_RS15345 0.43 -2.3 3,319,620 + RR42_RS15345 0.43 +0.9 3,319,621 - RR42_RS15345 0.43 +0.2 3,319,621 - RR42_RS15345 0.43 +1.6 3,319,621 - RR42_RS15345 0.43 -0.3 3,319,621 - RR42_RS15345 0.43 -0.3 3,319,621 - RR42_RS15345 0.43 +0.8
Or see this region's nucleotide sequence