Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15115 and RR42_RS15120 are separated by 108 nucleotides RR42_RS15120 and RR42_RS15125 are separated by 96 nucleotides
RR42_RS15115: RR42_RS15115 - threonine aldolase, at 3,271,209 to 3,272,240
_RS15115
RR42_RS15120: RR42_RS15120 - 4-oxalocrotonate tautomerase, at 3,272,349 to 3,272,537
_RS15120
RR42_RS15125: RR42_RS15125 - aldolase, at 3,272,634 to 3,273,407
_RS15125
Position (kb)
3272
3273 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3271.520 kb on + strand, within RR42_RS15115 at 3271.565 kb on - strand, within RR42_RS15115 at 3271.795 kb on + strand, within RR42_RS15115 at 3271.982 kb on + strand, within RR42_RS15115 at 3271.983 kb on - strand, within RR42_RS15115 at 3271.983 kb on - strand, within RR42_RS15115 at 3272.015 kb on - strand, within RR42_RS15115 at 3272.147 kb on - strand at 3272.215 kb on + strand at 3272.215 kb on + strand at 3272.216 kb on - strand at 3272.350 kb on + strand at 3272.350 kb on + strand at 3272.351 kb on - strand at 3272.351 kb on - strand at 3272.351 kb on - strand at 3272.417 kb on + strand, within RR42_RS15120 at 3272.520 kb on + strand at 3272.520 kb on + strand at 3272.521 kb on - strand at 3272.521 kb on - strand at 3272.521 kb on - strand at 3272.527 kb on + strand at 3272.661 kb on - strand at 3272.833 kb on + strand, within RR42_RS15125 at 3272.834 kb on - strand, within RR42_RS15125 at 3272.834 kb on - strand, within RR42_RS15125 at 3272.834 kb on - strand, within RR42_RS15125 at 3272.956 kb on + strand, within RR42_RS15125 at 3272.956 kb on + strand, within RR42_RS15125 at 3272.957 kb on - strand, within RR42_RS15125 at 3272.965 kb on + strand at 3272.980 kb on + strand, within RR42_RS15125 at 3273.054 kb on - strand, within RR42_RS15125 at 3273.122 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.266 kb on + strand, within RR42_RS15125 at 3273.267 kb on - strand, within RR42_RS15125 at 3273.274 kb on + strand, within RR42_RS15125 at 3273.274 kb on + strand, within RR42_RS15125 at 3273.274 kb on + strand, within RR42_RS15125 at 3273.274 kb on + strand, within RR42_RS15125 at 3273.275 kb on - strand, within RR42_RS15125 at 3273.275 kb on - strand, within RR42_RS15125 at 3273.480 kb on - strand at 3273.485 kb on - strand at 3273.485 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,271,520 + RR42_RS15115 0.30 -0.9 3,271,565 - RR42_RS15115 0.34 -0.8 3,271,795 + RR42_RS15115 0.57 +0.5 3,271,982 + RR42_RS15115 0.75 -3.7 3,271,983 - RR42_RS15115 0.75 +1.4 3,271,983 - RR42_RS15115 0.75 +1.4 3,272,015 - RR42_RS15115 0.78 -0.7 3,272,147 - -2.0 3,272,215 + +1.9 3,272,215 + +0.1 3,272,216 - -1.3 3,272,350 + -0.3 3,272,350 + +1.0 3,272,351 - -1.0 3,272,351 - -0.3 3,272,351 - -0.7 3,272,417 + RR42_RS15120 0.36 -2.2 3,272,520 + +0.9 3,272,520 + +1.4 3,272,521 - -1.3 3,272,521 - -0.2 3,272,521 - -1.5 3,272,527 + -1.0 3,272,661 - -0.2 3,272,833 + RR42_RS15125 0.26 +0.3 3,272,834 - RR42_RS15125 0.26 +0.7 3,272,834 - RR42_RS15125 0.26 +0.8 3,272,834 - RR42_RS15125 0.26 -2.8 3,272,956 + RR42_RS15125 0.42 -0.3 3,272,956 + RR42_RS15125 0.42 -1.2 3,272,957 - RR42_RS15125 0.42 +0.6 3,272,965 + -0.6 3,272,980 + RR42_RS15125 0.45 -0.6 3,273,054 - RR42_RS15125 0.54 -0.1 3,273,122 + RR42_RS15125 0.63 +1.6 3,273,266 + RR42_RS15125 0.82 +1.6 3,273,266 + RR42_RS15125 0.82 +0.9 3,273,266 + RR42_RS15125 0.82 +0.4 3,273,266 + RR42_RS15125 0.82 +0.6 3,273,266 + RR42_RS15125 0.82 -2.6 3,273,267 - RR42_RS15125 0.82 +0.4 3,273,274 + RR42_RS15125 0.83 -1.0 3,273,274 + RR42_RS15125 0.83 -3.1 3,273,274 + RR42_RS15125 0.83 -3.0 3,273,274 + RR42_RS15125 0.83 -1.2 3,273,275 - RR42_RS15125 0.83 -3.1 3,273,275 - RR42_RS15125 0.83 -0.0 3,273,480 - -1.3 3,273,485 - -0.2 3,273,485 - +0.3
Or see this region's nucleotide sequence