Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15045 and RR42_RS15050 are separated by 199 nucleotides RR42_RS15050 and RR42_RS15055 are separated by 223 nucleotides
RR42_RS15045: RR42_RS15045 - pyridine nucleotide-disulfide oxidoreductase, at 3,252,949 to 3,254,277
_RS15045
RR42_RS15050: RR42_RS15050 - DNA polymerase I, at 3,254,477 to 3,257,290
_RS15050
RR42_RS15055: RR42_RS15055 - hypothetical protein, at 3,257,514 to 3,258,233
_RS15055
Position (kb)
3254
3255
3256
3257
3258 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3253.662 kb on - strand, within RR42_RS15045 at 3253.686 kb on + strand, within RR42_RS15045 at 3253.687 kb on - strand, within RR42_RS15045 at 3253.687 kb on - strand, within RR42_RS15045 at 3253.687 kb on - strand, within RR42_RS15045 at 3254.069 kb on + strand, within RR42_RS15045 at 3254.070 kb on - strand, within RR42_RS15045 at 3254.070 kb on - strand, within RR42_RS15045 at 3254.070 kb on - strand, within RR42_RS15045 at 3254.070 kb on - strand, within RR42_RS15045 at 3254.136 kb on + strand, within RR42_RS15045 at 3254.136 kb on + strand, within RR42_RS15045 at 3254.137 kb on - strand, within RR42_RS15045 at 3254.137 kb on - strand, within RR42_RS15045 at 3254.137 kb on - strand, within RR42_RS15045 at 3254.137 kb on - strand, within RR42_RS15045 at 3254.161 kb on + strand at 3254.243 kb on - strand at 3254.243 kb on - strand at 3254.243 kb on - strand at 3254.283 kb on - strand at 3254.478 kb on + strand at 3254.478 kb on + strand at 3254.479 kb on - strand at 3254.905 kb on - strand, within RR42_RS15050 at 3254.905 kb on - strand, within RR42_RS15050 at 3254.968 kb on - strand, within RR42_RS15050 at 3254.997 kb on + strand, within RR42_RS15050 at 3255.116 kb on - strand, within RR42_RS15050 at 3255.346 kb on - strand, within RR42_RS15050 at 3255.346 kb on - strand, within RR42_RS15050 at 3255.688 kb on - strand, within RR42_RS15050 at 3255.694 kb on - strand, within RR42_RS15050 at 3255.694 kb on - strand, within RR42_RS15050 at 3255.727 kb on - strand, within RR42_RS15050 at 3255.921 kb on + strand, within RR42_RS15050 at 3255.922 kb on - strand, within RR42_RS15050 at 3255.993 kb on + strand, within RR42_RS15050 at 3255.993 kb on + strand, within RR42_RS15050 at 3256.084 kb on - strand, within RR42_RS15050 at 3256.131 kb on + strand, within RR42_RS15050 at 3256.131 kb on + strand, within RR42_RS15050 at 3256.132 kb on - strand, within RR42_RS15050 at 3256.132 kb on - strand, within RR42_RS15050 at 3257.125 kb on - strand at 3257.370 kb on - strand at 3257.474 kb on + strand at 3257.474 kb on + strand at 3257.474 kb on + strand at 3257.597 kb on + strand, within RR42_RS15055 at 3257.597 kb on + strand, within RR42_RS15055 at 3257.598 kb on - strand, within RR42_RS15055 at 3257.658 kb on + strand, within RR42_RS15055 at 3257.659 kb on - strand, within RR42_RS15055 at 3257.694 kb on + strand, within RR42_RS15055 at 3257.694 kb on + strand, within RR42_RS15055 at 3257.695 kb on - strand, within RR42_RS15055 at 3257.695 kb on - strand, within RR42_RS15055 at 3257.695 kb on - strand, within RR42_RS15055 at 3258.165 kb on + strand at 3258.165 kb on + strand at 3258.165 kb on + strand at 3258.165 kb on + strand at 3258.171 kb on + strand at 3258.174 kb on + strand at 3258.174 kb on + strand at 3258.174 kb on + strand at 3258.175 kb on - strand at 3258.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,253,662 - RR42_RS15045 0.54 -3.5 3,253,686 + RR42_RS15045 0.55 +0.4 3,253,687 - RR42_RS15045 0.56 -0.6 3,253,687 - RR42_RS15045 0.56 +0.1 3,253,687 - RR42_RS15045 0.56 -0.1 3,254,069 + RR42_RS15045 0.84 -1.3 3,254,070 - RR42_RS15045 0.84 -1.9 3,254,070 - RR42_RS15045 0.84 +0.1 3,254,070 - RR42_RS15045 0.84 -0.1 3,254,070 - RR42_RS15045 0.84 +0.6 3,254,136 + RR42_RS15045 0.89 -0.8 3,254,136 + RR42_RS15045 0.89 +0.4 3,254,137 - RR42_RS15045 0.89 +0.8 3,254,137 - RR42_RS15045 0.89 +0.4 3,254,137 - RR42_RS15045 0.89 +0.3 3,254,137 - RR42_RS15045 0.89 -0.6 3,254,161 + +0.4 3,254,243 - -0.9 3,254,243 - +1.0 3,254,243 - -0.3 3,254,283 - -0.7 3,254,478 + -0.9 3,254,478 + -0.4 3,254,479 - +0.8 3,254,905 - RR42_RS15050 0.15 -3.0 3,254,905 - RR42_RS15050 0.15 -2.6 3,254,968 - RR42_RS15050 0.17 -1.1 3,254,997 + RR42_RS15050 0.18 -2.5 3,255,116 - RR42_RS15050 0.23 -4.2 3,255,346 - RR42_RS15050 0.31 -3.2 3,255,346 - RR42_RS15050 0.31 -3.2 3,255,688 - RR42_RS15050 0.43 -0.0 3,255,694 - RR42_RS15050 0.43 -4.3 3,255,694 - RR42_RS15050 0.43 -1.6 3,255,727 - RR42_RS15050 0.44 -4.2 3,255,921 + RR42_RS15050 0.51 -3.9 3,255,922 - RR42_RS15050 0.51 -1.3 3,255,993 + RR42_RS15050 0.54 -1.3 3,255,993 + RR42_RS15050 0.54 -3.1 3,256,084 - RR42_RS15050 0.57 -2.6 3,256,131 + RR42_RS15050 0.59 -2.0 3,256,131 + RR42_RS15050 0.59 -1.9 3,256,132 - RR42_RS15050 0.59 -2.7 3,256,132 - RR42_RS15050 0.59 -0.6 3,257,125 - -1.8 3,257,370 - -1.7 3,257,474 + -0.9 3,257,474 + -1.0 3,257,474 + -0.4 3,257,597 + RR42_RS15055 0.12 -2.7 3,257,597 + RR42_RS15055 0.12 -0.1 3,257,598 - RR42_RS15055 0.12 +0.6 3,257,658 + RR42_RS15055 0.20 +1.0 3,257,659 - RR42_RS15055 0.20 -0.0 3,257,694 + RR42_RS15055 0.25 -0.0 3,257,694 + RR42_RS15055 0.25 +1.0 3,257,695 - RR42_RS15055 0.25 -0.2 3,257,695 - RR42_RS15055 0.25 -1.3 3,257,695 - RR42_RS15055 0.25 -0.2 3,258,165 + +0.3 3,258,165 + +0.6 3,258,165 + +0.2 3,258,165 + +0.1 3,258,171 + +0.1 3,258,174 + +0.5 3,258,174 + -1.8 3,258,174 + +0.6 3,258,175 - -0.5 3,258,226 - +1.6
Or see this region's nucleotide sequence