Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14755 and RR42_RS14760 are separated by 110 nucleotides RR42_RS14760 and RR42_RS14765 are separated by 19 nucleotides RR42_RS14765 and RR42_RS14770 are separated by 67 nucleotides RR42_RS14770 and RR42_RS14775 are separated by 195 nucleotides
RR42_RS14755: RR42_RS14755 - hypothetical protein, at 3,180,382 to 3,181,008
_RS14755
RR42_RS14760: RR42_RS14760 - molybdenum cofactor biosynthesis protein MogA, at 3,181,119 to 3,181,709
_RS14760
RR42_RS14765: RR42_RS14765 - transporter, at 3,181,729 to 3,182,610
_RS14765
RR42_RS14770: RR42_RS14770 - oligoribonuclease, at 3,182,678 to 3,183,328
_RS14770
RR42_RS14775: RR42_RS14775 - peptidase M48, at 3,183,524 to 3,184,771
_RS14775
Position (kb)
3181
3182
3183 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3180.934 kb on - strand, within RR42_RS14755 at 3181.123 kb on + strand at 3181.124 kb on - strand at 3181.164 kb on + strand at 3181.165 kb on - strand at 3181.165 kb on - strand at 3181.165 kb on - strand at 3181.214 kb on + strand, within RR42_RS14760 at 3181.215 kb on - strand, within RR42_RS14760 at 3181.609 kb on - strand, within RR42_RS14760 at 3181.609 kb on - strand, within RR42_RS14760 at 3181.635 kb on + strand, within RR42_RS14760 at 3181.635 kb on + strand, within RR42_RS14760 at 3181.745 kb on + strand at 3181.746 kb on - strand at 3181.746 kb on - strand at 3181.746 kb on - strand at 3182.547 kb on - strand at 3182.625 kb on + strand at 3183.413 kb on - strand at 3183.413 kb on - strand at 3183.413 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,180,934 - RR42_RS14755 0.88 -1.0 3,181,123 + +0.8 3,181,124 - +0.1 3,181,164 + +1.6 3,181,165 - +0.8 3,181,165 - -1.0 3,181,165 - +0.9 3,181,214 + RR42_RS14760 0.16 +0.6 3,181,215 - RR42_RS14760 0.16 +1.5 3,181,609 - RR42_RS14760 0.83 +0.1 3,181,609 - RR42_RS14760 0.83 -1.6 3,181,635 + RR42_RS14760 0.87 +0.5 3,181,635 + RR42_RS14760 0.87 +1.0 3,181,745 + +0.3 3,181,746 - +1.5 3,181,746 - +2.0 3,181,746 - -1.1 3,182,547 - -0.3 3,182,625 + -0.8 3,183,413 - +1.2 3,183,413 - -2.9 3,183,413 - +0.7
Or see this region's nucleotide sequence