Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14630 and RR42_RS14635 are separated by 182 nucleotides RR42_RS14635 and RR42_RS14640 are separated by 88 nucleotides
RR42_RS14630: RR42_RS14630 - MFS transporter, at 3,146,335 to 3,147,642
_RS14630
RR42_RS14635: RR42_RS14635 - proline-specific permease, at 3,147,825 to 3,149,225
_RS14635
RR42_RS14640: RR42_RS14640 - histidine ammonia-lyase, at 3,149,314 to 3,150,846
_RS14640
Position (kb)
3147
3148
3149
3150 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3146.831 kb on + strand, within RR42_RS14630 at 3146.831 kb on + strand, within RR42_RS14630 at 3146.831 kb on + strand, within RR42_RS14630 at 3146.832 kb on - strand, within RR42_RS14630 at 3146.843 kb on + strand, within RR42_RS14630 at 3146.844 kb on - strand, within RR42_RS14630 at 3146.844 kb on - strand, within RR42_RS14630 at 3146.846 kb on + strand, within RR42_RS14630 at 3146.847 kb on - strand, within RR42_RS14630 at 3146.847 kb on - strand, within RR42_RS14630 at 3146.856 kb on + strand, within RR42_RS14630 at 3146.856 kb on + strand, within RR42_RS14630 at 3146.857 kb on - strand, within RR42_RS14630 at 3146.857 kb on - strand, within RR42_RS14630 at 3146.857 kb on - strand, within RR42_RS14630 at 3146.857 kb on - strand, within RR42_RS14630 at 3146.858 kb on + strand, within RR42_RS14630 at 3146.859 kb on - strand, within RR42_RS14630 at 3146.859 kb on - strand, within RR42_RS14630 at 3147.153 kb on - strand, within RR42_RS14630 at 3147.153 kb on - strand, within RR42_RS14630 at 3147.153 kb on - strand, within RR42_RS14630 at 3147.180 kb on - strand, within RR42_RS14630 at 3147.287 kb on + strand, within RR42_RS14630 at 3147.288 kb on - strand, within RR42_RS14630 at 3147.377 kb on + strand, within RR42_RS14630 at 3147.377 kb on + strand, within RR42_RS14630 at 3147.377 kb on + strand, within RR42_RS14630 at 3147.377 kb on + strand, within RR42_RS14630 at 3147.479 kb on + strand, within RR42_RS14630 at 3147.479 kb on + strand, within RR42_RS14630 at 3147.480 kb on - strand, within RR42_RS14630 at 3147.480 kb on - strand, within RR42_RS14630 at 3147.512 kb on + strand at 3147.513 kb on - strand at 3147.513 kb on - strand at 3147.647 kb on + strand at 3147.647 kb on + strand at 3147.648 kb on - strand at 3147.663 kb on + strand at 3147.664 kb on - strand at 3147.722 kb on + strand at 3148.168 kb on + strand, within RR42_RS14635 at 3148.168 kb on + strand, within RR42_RS14635 at 3148.169 kb on - strand, within RR42_RS14635 at 3148.169 kb on - strand, within RR42_RS14635 at 3148.169 kb on - strand, within RR42_RS14635 at 3148.169 kb on - strand, within RR42_RS14635 at 3148.288 kb on + strand, within RR42_RS14635 at 3148.288 kb on + strand, within RR42_RS14635 at 3148.289 kb on - strand, within RR42_RS14635 at 3148.289 kb on - strand, within RR42_RS14635 at 3148.289 kb on - strand, within RR42_RS14635 at 3148.355 kb on + strand, within RR42_RS14635 at 3148.445 kb on - strand, within RR42_RS14635 at 3148.445 kb on - strand, within RR42_RS14635 at 3148.445 kb on - strand, within RR42_RS14635 at 3148.445 kb on - strand, within RR42_RS14635 at 3148.477 kb on + strand, within RR42_RS14635 at 3148.477 kb on + strand, within RR42_RS14635 at 3148.477 kb on + strand, within RR42_RS14635 at 3148.478 kb on - strand, within RR42_RS14635 at 3148.777 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.886 kb on - strand, within RR42_RS14635 at 3148.939 kb on + strand, within RR42_RS14635 at 3148.939 kb on + strand, within RR42_RS14635 at 3148.940 kb on - strand, within RR42_RS14635 at 3149.081 kb on - strand, within RR42_RS14635 at 3149.228 kb on - strand at 3149.420 kb on + strand at 3149.421 kb on - strand at 3149.423 kb on + strand at 3149.424 kb on - strand at 3149.754 kb on + strand, within RR42_RS14640 at 3149.755 kb on - strand, within RR42_RS14640 at 3150.058 kb on - strand, within RR42_RS14640 at 3150.077 kb on + strand, within RR42_RS14640 at 3150.077 kb on + strand, within RR42_RS14640 at 3150.078 kb on - strand, within RR42_RS14640 at 3150.078 kb on - strand, within RR42_RS14640 at 3150.184 kb on + strand, within RR42_RS14640
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,146,831 + RR42_RS14630 0.38 -3.1 3,146,831 + RR42_RS14630 0.38 +0.5 3,146,831 + RR42_RS14630 0.38 -1.7 3,146,832 - RR42_RS14630 0.38 +0.6 3,146,843 + RR42_RS14630 0.39 -1.3 3,146,844 - RR42_RS14630 0.39 +0.3 3,146,844 - RR42_RS14630 0.39 -0.4 3,146,846 + RR42_RS14630 0.39 -0.0 3,146,847 - RR42_RS14630 0.39 +0.4 3,146,847 - RR42_RS14630 0.39 +0.3 3,146,856 + RR42_RS14630 0.40 -0.6 3,146,856 + RR42_RS14630 0.40 +0.4 3,146,857 - RR42_RS14630 0.40 -0.5 3,146,857 - RR42_RS14630 0.40 -0.3 3,146,857 - RR42_RS14630 0.40 -1.2 3,146,857 - RR42_RS14630 0.40 +1.0 3,146,858 + RR42_RS14630 0.40 -0.2 3,146,859 - RR42_RS14630 0.40 -3.2 3,146,859 - RR42_RS14630 0.40 -0.9 3,147,153 - RR42_RS14630 0.63 +0.5 3,147,153 - RR42_RS14630 0.63 -0.8 3,147,153 - RR42_RS14630 0.63 -0.5 3,147,180 - RR42_RS14630 0.65 -0.4 3,147,287 + RR42_RS14630 0.73 -1.0 3,147,288 - RR42_RS14630 0.73 +0.0 3,147,377 + RR42_RS14630 0.80 +1.0 3,147,377 + RR42_RS14630 0.80 -0.3 3,147,377 + RR42_RS14630 0.80 +1.1 3,147,377 + RR42_RS14630 0.80 -0.6 3,147,479 + RR42_RS14630 0.87 -0.3 3,147,479 + RR42_RS14630 0.87 -2.2 3,147,480 - RR42_RS14630 0.88 +0.3 3,147,480 - RR42_RS14630 0.88 +0.6 3,147,512 + +0.4 3,147,513 - +0.1 3,147,513 - +0.3 3,147,647 + +0.8 3,147,647 + +0.4 3,147,648 - +0.8 3,147,663 + +2.0 3,147,664 - +0.2 3,147,722 + -0.4 3,148,168 + RR42_RS14635 0.24 -1.2 3,148,168 + RR42_RS14635 0.24 +0.7 3,148,169 - RR42_RS14635 0.25 -0.6 3,148,169 - RR42_RS14635 0.25 -0.2 3,148,169 - RR42_RS14635 0.25 +1.5 3,148,169 - RR42_RS14635 0.25 -0.8 3,148,288 + RR42_RS14635 0.33 -2.8 3,148,288 + RR42_RS14635 0.33 -0.6 3,148,289 - RR42_RS14635 0.33 -2.6 3,148,289 - RR42_RS14635 0.33 +0.0 3,148,289 - RR42_RS14635 0.33 +0.5 3,148,355 + RR42_RS14635 0.38 -1.3 3,148,445 - RR42_RS14635 0.44 +0.0 3,148,445 - RR42_RS14635 0.44 -1.8 3,148,445 - RR42_RS14635 0.44 +0.0 3,148,445 - RR42_RS14635 0.44 +0.2 3,148,477 + RR42_RS14635 0.47 +0.1 3,148,477 + RR42_RS14635 0.47 +0.6 3,148,477 + RR42_RS14635 0.47 -0.4 3,148,478 - RR42_RS14635 0.47 +1.1 3,148,777 + RR42_RS14635 0.68 +1.5 3,148,885 + RR42_RS14635 0.76 -0.7 3,148,885 + RR42_RS14635 0.76 -0.7 3,148,885 + RR42_RS14635 0.76 -0.2 3,148,885 + RR42_RS14635 0.76 +0.8 3,148,885 + RR42_RS14635 0.76 -1.0 3,148,886 - RR42_RS14635 0.76 +2.2 3,148,939 + RR42_RS14635 0.80 -0.7 3,148,939 + RR42_RS14635 0.80 +0.8 3,148,940 - RR42_RS14635 0.80 +1.0 3,149,081 - RR42_RS14635 0.90 -0.2 3,149,228 - -0.2 3,149,420 + +0.3 3,149,421 - -2.3 3,149,423 + +0.1 3,149,424 - -1.1 3,149,754 + RR42_RS14640 0.29 -1.5 3,149,755 - RR42_RS14640 0.29 -0.6 3,150,058 - RR42_RS14640 0.49 +0.3 3,150,077 + RR42_RS14640 0.50 -1.1 3,150,077 + RR42_RS14640 0.50 +0.7 3,150,078 - RR42_RS14640 0.50 -2.8 3,150,078 - RR42_RS14640 0.50 -1.3 3,150,184 + RR42_RS14640 0.57 +0.9
Or see this region's nucleotide sequence