Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14270 and RR42_RS14275 are separated by 129 nucleotides RR42_RS14275 and RR42_RS14280 are separated by 34 nucleotides RR42_RS14280 and RR42_RS14285 are separated by 390 nucleotides RR42_RS14285 and RR42_RS14290 are separated by 97 nucleotides RR42_RS14290 and RR42_RS14295 are separated by 205 nucleotides
RR42_RS14270: RR42_RS14270 - tRNA-Met, at 3,068,887 to 3,068,962
_RS14270
RR42_RS14275: RR42_RS14275 - cold-shock protein, at 3,069,092 to 3,069,289
_RS14275
RR42_RS14280: RR42_RS14280 - 30S ribosomal protein S21, at 3,069,324 to 3,069,536
_RS14280
RR42_RS14285: RR42_RS14285 - lysine transporter LysE, at 3,069,927 to 3,070,544
_RS14285
RR42_RS14290: RR42_RS14290 - thioesterase, at 3,070,642 to 3,071,175
_RS14290
RR42_RS14295: RR42_RS14295 - ABC transporter ATP-binding protein, at 3,071,381 to 3,072,292
_RS14295
Position (kb)
3069
3070
3071 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3068.977 kb on + strand at 3068.977 kb on + strand at 3068.977 kb on + strand at 3068.977 kb on + strand at 3069.002 kb on + strand at 3069.589 kb on - strand at 3069.590 kb on + strand at 3069.793 kb on + strand at 3069.794 kb on - strand at 3069.794 kb on - strand at 3069.794 kb on - strand at 3069.794 kb on - strand at 3069.794 kb on - strand at 3069.794 kb on - strand at 3069.813 kb on - strand at 3069.847 kb on - strand at 3069.847 kb on - strand at 3069.875 kb on + strand at 3069.875 kb on + strand at 3069.875 kb on + strand at 3069.875 kb on + strand at 3069.987 kb on + strand at 3069.988 kb on - strand at 3069.988 kb on - strand at 3070.014 kb on - strand, within RR42_RS14285 at 3070.048 kb on + strand, within RR42_RS14285 at 3070.292 kb on - strand, within RR42_RS14285 at 3070.300 kb on + strand, within RR42_RS14285 at 3070.301 kb on - strand, within RR42_RS14285 at 3070.427 kb on - strand, within RR42_RS14285 at 3070.427 kb on - strand, within RR42_RS14285 at 3070.505 kb on + strand at 3070.506 kb on - strand at 3070.506 kb on - strand at 3070.593 kb on + strand at 3070.595 kb on + strand at 3070.596 kb on - strand at 3070.596 kb on - strand at 3070.596 kb on - strand at 3070.596 kb on - strand at 3070.596 kb on - strand at 3070.640 kb on + strand at 3070.640 kb on + strand at 3070.641 kb on - strand at 3070.677 kb on + strand at 3070.685 kb on + strand at 3070.686 kb on - strand at 3070.686 kb on - strand at 3070.691 kb on + strand at 3070.808 kb on + strand, within RR42_RS14290 at 3070.808 kb on + strand, within RR42_RS14290 at 3070.809 kb on - strand, within RR42_RS14290 at 3070.809 kb on - strand, within RR42_RS14290 at 3070.836 kb on - strand, within RR42_RS14290 at 3070.836 kb on - strand, within RR42_RS14290 at 3070.904 kb on + strand, within RR42_RS14290 at 3070.904 kb on + strand, within RR42_RS14290 at 3071.274 kb on + strand at 3071.274 kb on + strand at 3071.334 kb on + strand at 3071.335 kb on - strand at 3071.344 kb on + strand at 3071.345 kb on - strand at 3071.345 kb on - strand at 3071.400 kb on + strand at 3071.400 kb on + strand at 3071.409 kb on + strand at 3071.409 kb on + strand at 3071.409 kb on + strand at 3071.409 kb on + strand at 3071.409 kb on + strand at 3071.409 kb on + strand at 3071.409 kb on + strand at 3071.410 kb on - strand at 3071.410 kb on - strand at 3071.410 kb on - strand at 3071.421 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,068,977 + -0.9 3,068,977 + -0.9 3,068,977 + -2.8 3,068,977 + -2.0 3,069,002 + +0.4 3,069,589 - -2.2 3,069,590 + -3.5 3,069,793 + -0.6 3,069,794 - +0.7 3,069,794 - +0.9 3,069,794 - +1.2 3,069,794 - -0.6 3,069,794 - -0.4 3,069,794 - -1.0 3,069,813 - -0.4 3,069,847 - +0.5 3,069,847 - +1.4 3,069,875 + +0.8 3,069,875 + +0.6 3,069,875 + -1.6 3,069,875 + -0.6 3,069,987 + +0.2 3,069,988 - +0.4 3,069,988 - -0.5 3,070,014 - RR42_RS14285 0.14 -2.0 3,070,048 + RR42_RS14285 0.20 -1.4 3,070,292 - RR42_RS14285 0.59 -0.3 3,070,300 + RR42_RS14285 0.60 +1.0 3,070,301 - RR42_RS14285 0.61 +0.1 3,070,427 - RR42_RS14285 0.81 -0.7 3,070,427 - RR42_RS14285 0.81 -0.4 3,070,505 + +1.2 3,070,506 - +0.5 3,070,506 - -0.3 3,070,593 + +0.9 3,070,595 + -1.4 3,070,596 - -0.9 3,070,596 - +0.3 3,070,596 - -0.4 3,070,596 - -3.4 3,070,596 - -0.9 3,070,640 + -0.6 3,070,640 + +1.2 3,070,641 - +1.6 3,070,677 + +0.1 3,070,685 + -1.2 3,070,686 - +1.0 3,070,686 - +0.5 3,070,691 + +1.0 3,070,808 + RR42_RS14290 0.31 -0.1 3,070,808 + RR42_RS14290 0.31 +0.1 3,070,809 - RR42_RS14290 0.31 -0.3 3,070,809 - RR42_RS14290 0.31 -0.3 3,070,836 - RR42_RS14290 0.36 -0.9 3,070,836 - RR42_RS14290 0.36 +1.1 3,070,904 + RR42_RS14290 0.49 -0.1 3,070,904 + RR42_RS14290 0.49 +1.2 3,071,274 + +0.8 3,071,274 + -1.1 3,071,334 + +0.3 3,071,335 - +2.6 3,071,344 + +1.1 3,071,345 - -1.6 3,071,345 - -0.1 3,071,400 + +0.1 3,071,400 + +0.9 3,071,409 + +1.1 3,071,409 + -1.6 3,071,409 + -1.0 3,071,409 + -0.0 3,071,409 + -0.7 3,071,409 + +0.5 3,071,409 + +0.8 3,071,410 - -0.1 3,071,410 - +0.4 3,071,410 - -0.4 3,071,421 - -0.0
Or see this region's nucleotide sequence