Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14115 and RR42_RS14120 are separated by 323 nucleotides RR42_RS14120 and RR42_RS14125 are separated by 119 nucleotides RR42_RS14125 and RR42_RS14130 are separated by 248 nucleotides RR42_RS14130 and RR42_RS14135 are separated by 51 nucleotides
RR42_RS14115: RR42_RS14115 - phosphate acyltransferase, at 3,036,266 to 3,037,342
_RS14115
RR42_RS14120: RR42_RS14120 - 50S ribosomal protein L32, at 3,037,666 to 3,037,845
_RS14120
RR42_RS14125: RR42_RS14125 - metal-binding protein, at 3,037,965 to 3,038,561
_RS14125
RR42_RS14130: RR42_RS14130 - septum formation inhibitor Maf, at 3,038,810 to 3,039,409
_RS14130
RR42_RS14135: RR42_RS14135 - SAM-dependent methyltransferase, at 3,039,461 to 3,040,192
_RS14135
Position (kb)
3037
3038
3039 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3037.499 kb on - strand at 3037.499 kb on - strand at 3037.622 kb on - strand at 3037.622 kb on - strand at 3037.622 kb on - strand at 3038.279 kb on - strand, within RR42_RS14125 at 3038.405 kb on - strand, within RR42_RS14125 at 3038.567 kb on - strand at 3038.622 kb on - strand at 3038.626 kb on - strand at 3038.626 kb on - strand at 3038.626 kb on - strand at 3038.700 kb on - strand at 3038.736 kb on - strand at 3038.805 kb on + strand at 3038.805 kb on + strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.809 kb on + strand at 3038.809 kb on + strand at 3038.809 kb on + strand at 3038.809 kb on + strand at 3038.810 kb on - strand at 3038.810 kb on - strand at 3038.810 kb on - strand at 3039.236 kb on + strand, within RR42_RS14130 at 3039.236 kb on + strand, within RR42_RS14130 at 3039.236 kb on + strand, within RR42_RS14130 at 3039.236 kb on + strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.257 kb on + strand, within RR42_RS14130 at 3039.257 kb on + strand, within RR42_RS14130 at 3039.257 kb on + strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.313 kb on + strand, within RR42_RS14130 at 3039.313 kb on + strand, within RR42_RS14130 at 3039.342 kb on + strand, within RR42_RS14130 at 3039.343 kb on - strand, within RR42_RS14130 at 3039.370 kb on + strand at 3039.370 kb on + strand at 3039.370 kb on + strand at 3039.370 kb on + strand at 3039.370 kb on + strand at 3039.371 kb on - strand at 3039.371 kb on - strand at 3039.477 kb on - strand at 3039.477 kb on - strand at 3039.477 kb on - strand at 3039.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,037,499 - -0.8 3,037,499 - +0.9 3,037,622 - -3.5 3,037,622 - -3.0 3,037,622 - -3.0 3,038,279 - RR42_RS14125 0.53 -3.0 3,038,405 - RR42_RS14125 0.74 -0.9 3,038,567 - -1.2 3,038,622 - -1.3 3,038,626 - -2.3 3,038,626 - -0.3 3,038,626 - -0.0 3,038,700 - -1.6 3,038,736 - +1.2 3,038,805 + -3.2 3,038,805 + -0.1 3,038,806 - -1.6 3,038,806 - -0.1 3,038,806 - -0.3 3,038,806 - +1.1 3,038,806 - +0.2 3,038,806 - -0.9 3,038,809 + +0.4 3,038,809 + -3.2 3,038,809 + -1.1 3,038,809 + -1.3 3,038,810 - +0.3 3,038,810 - +1.0 3,038,810 - +0.3 3,039,236 + RR42_RS14130 0.71 +0.3 3,039,236 + RR42_RS14130 0.71 -0.0 3,039,236 + RR42_RS14130 0.71 -0.3 3,039,236 + RR42_RS14130 0.71 -0.1 3,039,237 - RR42_RS14130 0.71 +1.4 3,039,237 - RR42_RS14130 0.71 +1.0 3,039,237 - RR42_RS14130 0.71 +1.4 3,039,237 - RR42_RS14130 0.71 +0.5 3,039,237 - RR42_RS14130 0.71 -0.0 3,039,257 + RR42_RS14130 0.74 -0.9 3,039,257 + RR42_RS14130 0.74 +0.2 3,039,257 + RR42_RS14130 0.74 -0.3 3,039,258 - RR42_RS14130 0.75 -1.3 3,039,258 - RR42_RS14130 0.75 +0.1 3,039,258 - RR42_RS14130 0.75 -2.2 3,039,258 - RR42_RS14130 0.75 -0.1 3,039,258 - RR42_RS14130 0.75 +0.5 3,039,258 - RR42_RS14130 0.75 -1.2 3,039,258 - RR42_RS14130 0.75 +0.2 3,039,313 + RR42_RS14130 0.84 -0.5 3,039,313 + RR42_RS14130 0.84 -0.2 3,039,342 + RR42_RS14130 0.89 +1.7 3,039,343 - RR42_RS14130 0.89 -0.2 3,039,370 + -0.4 3,039,370 + +0.3 3,039,370 + +0.6 3,039,370 + -0.9 3,039,370 + -0.1 3,039,371 - +0.8 3,039,371 - -1.7 3,039,477 - +1.4 3,039,477 - +1.0 3,039,477 - -0.3 3,039,477 - +0.3
Or see this region's nucleotide sequence