Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS14125

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14115 and RR42_RS14120 are separated by 323 nucleotidesRR42_RS14120 and RR42_RS14125 are separated by 119 nucleotidesRR42_RS14125 and RR42_RS14130 are separated by 248 nucleotidesRR42_RS14130 and RR42_RS14135 are separated by 51 nucleotides RR42_RS14115: RR42_RS14115 - phosphate acyltransferase, at 3,036,266 to 3,037,342 _RS14115 RR42_RS14120: RR42_RS14120 - 50S ribosomal protein L32, at 3,037,666 to 3,037,845 _RS14120 RR42_RS14125: RR42_RS14125 - metal-binding protein, at 3,037,965 to 3,038,561 _RS14125 RR42_RS14130: RR42_RS14130 - septum formation inhibitor Maf, at 3,038,810 to 3,039,409 _RS14130 RR42_RS14135: RR42_RS14135 - SAM-dependent methyltransferase, at 3,039,461 to 3,040,192 _RS14135 Position (kb) 3037 3038 3039Strain fitness (log2 ratio) -3 -2 -1 0 1at 3037.499 kb on - strandat 3037.499 kb on - strandat 3037.622 kb on - strandat 3037.622 kb on - strandat 3037.622 kb on - strandat 3038.279 kb on - strand, within RR42_RS14125at 3038.405 kb on - strand, within RR42_RS14125at 3038.567 kb on - strandat 3038.622 kb on - strandat 3038.626 kb on - strandat 3038.626 kb on - strandat 3038.626 kb on - strandat 3038.700 kb on - strandat 3038.736 kb on - strandat 3038.805 kb on + strandat 3038.805 kb on + strandat 3038.806 kb on - strandat 3038.806 kb on - strandat 3038.806 kb on - strandat 3038.806 kb on - strandat 3038.806 kb on - strandat 3038.806 kb on - strandat 3038.809 kb on + strandat 3038.809 kb on + strandat 3038.809 kb on + strandat 3038.809 kb on + strandat 3038.810 kb on - strandat 3038.810 kb on - strandat 3038.810 kb on - strandat 3039.236 kb on + strand, within RR42_RS14130at 3039.236 kb on + strand, within RR42_RS14130at 3039.236 kb on + strand, within RR42_RS14130at 3039.236 kb on + strand, within RR42_RS14130at 3039.237 kb on - strand, within RR42_RS14130at 3039.237 kb on - strand, within RR42_RS14130at 3039.237 kb on - strand, within RR42_RS14130at 3039.237 kb on - strand, within RR42_RS14130at 3039.237 kb on - strand, within RR42_RS14130at 3039.257 kb on + strand, within RR42_RS14130at 3039.257 kb on + strand, within RR42_RS14130at 3039.257 kb on + strand, within RR42_RS14130at 3039.258 kb on - strand, within RR42_RS14130at 3039.258 kb on - strand, within RR42_RS14130at 3039.258 kb on - strand, within RR42_RS14130at 3039.258 kb on - strand, within RR42_RS14130at 3039.258 kb on - strand, within RR42_RS14130at 3039.258 kb on - strand, within RR42_RS14130at 3039.258 kb on - strand, within RR42_RS14130at 3039.313 kb on + strand, within RR42_RS14130at 3039.313 kb on + strand, within RR42_RS14130at 3039.342 kb on + strand, within RR42_RS14130at 3039.343 kb on - strand, within RR42_RS14130at 3039.370 kb on + strandat 3039.370 kb on + strandat 3039.370 kb on + strandat 3039.370 kb on + strandat 3039.370 kb on + strandat 3039.371 kb on - strandat 3039.371 kb on - strandat 3039.477 kb on - strandat 3039.477 kb on - strandat 3039.477 kb on - strandat 3039.477 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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3,037,499 - -0.8
3,037,499 - +0.9
3,037,622 - -3.5
3,037,622 - -3.0
3,037,622 - -3.0
3,038,279 - RR42_RS14125 0.53 -3.0
3,038,405 - RR42_RS14125 0.74 -0.9
3,038,567 - -1.2
3,038,622 - -1.3
3,038,626 - -2.3
3,038,626 - -0.3
3,038,626 - -0.0
3,038,700 - -1.6
3,038,736 - +1.2
3,038,805 + -3.2
3,038,805 + -0.1
3,038,806 - -1.6
3,038,806 - -0.1
3,038,806 - -0.3
3,038,806 - +1.1
3,038,806 - +0.2
3,038,806 - -0.9
3,038,809 + +0.4
3,038,809 + -3.2
3,038,809 + -1.1
3,038,809 + -1.3
3,038,810 - +0.3
3,038,810 - +1.0
3,038,810 - +0.3
3,039,236 + RR42_RS14130 0.71 +0.3
3,039,236 + RR42_RS14130 0.71 -0.0
3,039,236 + RR42_RS14130 0.71 -0.3
3,039,236 + RR42_RS14130 0.71 -0.1
3,039,237 - RR42_RS14130 0.71 +1.4
3,039,237 - RR42_RS14130 0.71 +1.0
3,039,237 - RR42_RS14130 0.71 +1.4
3,039,237 - RR42_RS14130 0.71 +0.5
3,039,237 - RR42_RS14130 0.71 -0.0
3,039,257 + RR42_RS14130 0.74 -0.9
3,039,257 + RR42_RS14130 0.74 +0.2
3,039,257 + RR42_RS14130 0.74 -0.3
3,039,258 - RR42_RS14130 0.75 -1.3
3,039,258 - RR42_RS14130 0.75 +0.1
3,039,258 - RR42_RS14130 0.75 -2.2
3,039,258 - RR42_RS14130 0.75 -0.1
3,039,258 - RR42_RS14130 0.75 +0.5
3,039,258 - RR42_RS14130 0.75 -1.2
3,039,258 - RR42_RS14130 0.75 +0.2
3,039,313 + RR42_RS14130 0.84 -0.5
3,039,313 + RR42_RS14130 0.84 -0.2
3,039,342 + RR42_RS14130 0.89 +1.7
3,039,343 - RR42_RS14130 0.89 -0.2
3,039,370 + -0.4
3,039,370 + +0.3
3,039,370 + +0.6
3,039,370 + -0.9
3,039,370 + -0.1
3,039,371 - +0.8
3,039,371 - -1.7
3,039,477 - +1.4
3,039,477 - +1.0
3,039,477 - -0.3
3,039,477 - +0.3

Or see this region's nucleotide sequence