Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS13915

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS13905 and RR42_RS13910 are separated by 96 nucleotidesRR42_RS13910 and RR42_RS13915 are separated by 21 nucleotidesRR42_RS13915 and RR42_RS13920 are separated by 448 nucleotides RR42_RS13905: RR42_RS13905 - fumarate hydratase, at 2,997,194 to 2,998,717 _RS13905 RR42_RS13910: RR42_RS13910 - glutamate racemase, at 2,998,814 to 2,999,626 _RS13910 RR42_RS13915: RR42_RS13915 - ATPase, at 2,999,648 to 3,001,948 _RS13915 RR42_RS13920: RR42_RS13920 - energy transducer TonB, at 3,002,397 to 3,003,053 _RS13920 Position (kb) 2999 3000 3001 3002Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 2998.910 kb on + strand, within RR42_RS13910at 2998.910 kb on + strand, within RR42_RS13910at 2998.911 kb on - strand, within RR42_RS13910at 2999.312 kb on + strand, within RR42_RS13910at 2999.352 kb on + strand, within RR42_RS13910at 2999.352 kb on + strand, within RR42_RS13910at 2999.353 kb on - strand, within RR42_RS13910at 2999.353 kb on - strand, within RR42_RS13910at 2999.353 kb on - strand, within RR42_RS13910at 2999.353 kb on - strand, within RR42_RS13910at 2999.516 kb on + strand, within RR42_RS13910at 2999.517 kb on - strand, within RR42_RS13910at 2999.777 kb on - strandat 2999.978 kb on + strand, within RR42_RS13915at 2999.978 kb on + strand, within RR42_RS13915at 2999.978 kb on + strand, within RR42_RS13915at 3000.039 kb on + strand, within RR42_RS13915at 3000.039 kb on + strand, within RR42_RS13915at 3000.039 kb on + strand, within RR42_RS13915at 3000.040 kb on - strand, within RR42_RS13915at 3000.040 kb on - strand, within RR42_RS13915at 3000.040 kb on - strand, within RR42_RS13915at 3000.040 kb on - strand, within RR42_RS13915at 3000.040 kb on - strand, within RR42_RS13915at 3000.162 kb on + strand, within RR42_RS13915at 3000.163 kb on - strand, within RR42_RS13915at 3000.265 kb on - strand, within RR42_RS13915at 3000.265 kb on - strand, within RR42_RS13915at 3000.265 kb on - strand, within RR42_RS13915at 3000.265 kb on - strand, within RR42_RS13915at 3000.265 kb on - strand, within RR42_RS13915at 3000.265 kb on - strand, within RR42_RS13915at 3000.345 kb on + strand, within RR42_RS13915at 3000.345 kb on + strand, within RR42_RS13915at 3000.346 kb on - strand, within RR42_RS13915at 3000.358 kb on - strand, within RR42_RS13915at 3000.434 kb on + strand, within RR42_RS13915at 3000.544 kb on + strand, within RR42_RS13915at 3000.544 kb on + strand, within RR42_RS13915at 3000.545 kb on - strand, within RR42_RS13915at 3000.545 kb on - strand, within RR42_RS13915at 3000.652 kb on - strand, within RR42_RS13915at 3000.652 kb on - strand, within RR42_RS13915at 3000.939 kb on + strand, within RR42_RS13915at 3001.009 kb on - strand, within RR42_RS13915at 3001.009 kb on - strand, within RR42_RS13915at 3001.009 kb on - strand, within RR42_RS13915at 3001.071 kb on + strand, within RR42_RS13915at 3001.071 kb on + strand, within RR42_RS13915at 3001.072 kb on - strand, within RR42_RS13915at 3001.099 kb on - strand, within RR42_RS13915at 3001.170 kb on + strand, within RR42_RS13915at 3001.170 kb on + strand, within RR42_RS13915at 3001.268 kb on - strand, within RR42_RS13915at 3001.268 kb on - strand, within RR42_RS13915at 3001.370 kb on + strand, within RR42_RS13915at 3001.410 kb on + strand, within RR42_RS13915at 3001.410 kb on + strand, within RR42_RS13915at 3001.429 kb on - strand, within RR42_RS13915at 3001.489 kb on + strand, within RR42_RS13915at 3001.490 kb on - strand, within RR42_RS13915at 3001.490 kb on - strand, within RR42_RS13915at 3001.490 kb on - strand, within RR42_RS13915at 3001.564 kb on + strand, within RR42_RS13915at 3001.564 kb on + strand, within RR42_RS13915at 3001.564 kb on + strand, within RR42_RS13915at 3001.565 kb on - strand, within RR42_RS13915at 3001.565 kb on - strand, within RR42_RS13915at 3001.587 kb on + strand, within RR42_RS13915at 3001.588 kb on - strand, within RR42_RS13915at 3001.588 kb on - strand, within RR42_RS13915at 3001.588 kb on - strand, within RR42_RS13915at 3001.599 kb on + strand, within RR42_RS13915at 3001.700 kb on - strand, within RR42_RS13915at 3001.795 kb on + strandat 3001.795 kb on + strandat 3001.796 kb on - strandat 3002.029 kb on - strandat 3002.034 kb on - strandat 3002.116 kb on - strandat 3002.132 kb on - strandat 3002.133 kb on + strandat 3002.133 kb on + strandat 3002.270 kb on + strandat 3002.288 kb on + strandat 3002.288 kb on + strandat 3002.288 kb on + strandat 3002.288 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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2,998,910 + RR42_RS13910 0.12 -4.7
2,998,910 + RR42_RS13910 0.12 -4.6
2,998,911 - RR42_RS13910 0.12 -2.6
2,999,312 + RR42_RS13910 0.61 -2.8
2,999,352 + RR42_RS13910 0.66 -4.0
2,999,352 + RR42_RS13910 0.66 -4.9
2,999,353 - RR42_RS13910 0.66 -1.6
2,999,353 - RR42_RS13910 0.66 -3.3
2,999,353 - RR42_RS13910 0.66 -2.7
2,999,353 - RR42_RS13910 0.66 -1.3
2,999,516 + RR42_RS13910 0.86 -3.8
2,999,517 - RR42_RS13910 0.86 -2.8
2,999,777 - -1.8
2,999,978 + RR42_RS13915 0.14 +0.1
2,999,978 + RR42_RS13915 0.14 +1.3
2,999,978 + RR42_RS13915 0.14 -0.2
3,000,039 + RR42_RS13915 0.17 -0.6
3,000,039 + RR42_RS13915 0.17 -0.3
3,000,039 + RR42_RS13915 0.17 -0.3
3,000,040 - RR42_RS13915 0.17 +0.7
3,000,040 - RR42_RS13915 0.17 +0.3
3,000,040 - RR42_RS13915 0.17 +3.0
3,000,040 - RR42_RS13915 0.17 -0.3
3,000,040 - RR42_RS13915 0.17 +0.7
3,000,162 + RR42_RS13915 0.22 +0.9
3,000,163 - RR42_RS13915 0.22 +0.9
3,000,265 - RR42_RS13915 0.27 +2.8
3,000,265 - RR42_RS13915 0.27 +1.0
3,000,265 - RR42_RS13915 0.27 +1.0
3,000,265 - RR42_RS13915 0.27 +1.3
3,000,265 - RR42_RS13915 0.27 +0.4
3,000,265 - RR42_RS13915 0.27 +1.4
3,000,345 + RR42_RS13915 0.30 +0.8
3,000,345 + RR42_RS13915 0.30 +0.8
3,000,346 - RR42_RS13915 0.30 +2.2
3,000,358 - RR42_RS13915 0.31 +2.1
3,000,434 + RR42_RS13915 0.34 -1.3
3,000,544 + RR42_RS13915 0.39 -0.0
3,000,544 + RR42_RS13915 0.39 +0.6
3,000,545 - RR42_RS13915 0.39 +0.6
3,000,545 - RR42_RS13915 0.39 +1.9
3,000,652 - RR42_RS13915 0.44 +0.4
3,000,652 - RR42_RS13915 0.44 +0.8
3,000,939 + RR42_RS13915 0.56 -0.0
3,001,009 - RR42_RS13915 0.59 +1.9
3,001,009 - RR42_RS13915 0.59 +1.6
3,001,009 - RR42_RS13915 0.59 -0.6
3,001,071 + RR42_RS13915 0.62 -0.0
3,001,071 + RR42_RS13915 0.62 +1.6
3,001,072 - RR42_RS13915 0.62 +2.8
3,001,099 - RR42_RS13915 0.63 +1.6
3,001,170 + RR42_RS13915 0.66 +0.3
3,001,170 + RR42_RS13915 0.66 +0.8
3,001,268 - RR42_RS13915 0.70 +2.2
3,001,268 - RR42_RS13915 0.70 +1.6
3,001,370 + RR42_RS13915 0.75 -0.1
3,001,410 + RR42_RS13915 0.77 +0.5
3,001,410 + RR42_RS13915 0.77 +2.1
3,001,429 - RR42_RS13915 0.77 +2.1
3,001,489 + RR42_RS13915 0.80 +0.6
3,001,490 - RR42_RS13915 0.80 +2.4
3,001,490 - RR42_RS13915 0.80 +3.4
3,001,490 - RR42_RS13915 0.80 +2.7
3,001,564 + RR42_RS13915 0.83 +0.7
3,001,564 + RR42_RS13915 0.83 +2.5
3,001,564 + RR42_RS13915 0.83 +0.6
3,001,565 - RR42_RS13915 0.83 +3.7
3,001,565 - RR42_RS13915 0.83 +2.7
3,001,587 + RR42_RS13915 0.84 +0.4
3,001,588 - RR42_RS13915 0.84 +3.8
3,001,588 - RR42_RS13915 0.84 +2.9
3,001,588 - RR42_RS13915 0.84 +2.2
3,001,599 + RR42_RS13915 0.85 +0.5
3,001,700 - RR42_RS13915 0.89 +4.0
3,001,795 + -1.0
3,001,795 + -0.2
3,001,796 - +3.2
3,002,029 - +3.9
3,002,034 - +2.9
3,002,116 - +2.7
3,002,132 - +3.2
3,002,133 + -0.3
3,002,133 + -0.5
3,002,270 + +0.7
3,002,288 + +1.6
3,002,288 + +3.8
3,002,288 + +2.6
3,002,288 + +2.0

Or see this region's nucleotide sequence