Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13830 and RR42_RS13835 are separated by 127 nucleotides RR42_RS13835 and RR42_RS13840 are separated by 181 nucleotides RR42_RS13840 and RR42_RS13845 are separated by 102 nucleotides RR42_RS13845 and RR42_RS13850 are separated by 118 nucleotides RR42_RS13850 and RR42_RS13855 are separated by 82 nucleotides
RR42_RS13830: RR42_RS13830 - hypothetical protein, at 2,983,898 to 2,984,182
_RS13830
RR42_RS13835: RR42_RS13835 - hypothetical protein, at 2,984,310 to 2,984,537
_RS13835
RR42_RS13840: RR42_RS13840 - serine hydrolase, at 2,984,719 to 2,986,011
_RS13840
RR42_RS13845: RR42_RS13845 - hypothetical protein, at 2,986,114 to 2,986,398
_RS13845
RR42_RS13850: RR42_RS13850 - hypothetical protein, at 2,986,517 to 2,986,735
_RS13850
RR42_RS13855: RR42_RS13855 - hypothetical protein, at 2,986,818 to 2,987,021
_RS13855
Position (kb)
2984
2985
2986
2987 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2983.742 kb on + strand at 2983.742 kb on + strand at 2983.745 kb on - strand at 2984.095 kb on + strand, within RR42_RS13830 at 2984.195 kb on + strand at 2984.196 kb on - strand at 2984.196 kb on - strand at 2984.215 kb on - strand at 2984.219 kb on + strand at 2984.219 kb on + strand at 2984.219 kb on + strand at 2984.220 kb on - strand at 2984.220 kb on - strand at 2984.220 kb on - strand at 2984.220 kb on - strand at 2984.220 kb on - strand at 2984.365 kb on + strand, within RR42_RS13835 at 2984.366 kb on - strand, within RR42_RS13835 at 2984.507 kb on - strand, within RR42_RS13835 at 2984.566 kb on + strand at 2984.567 kb on - strand at 2984.698 kb on + strand at 2984.721 kb on - strand at 2984.744 kb on + strand at 2984.744 kb on + strand at 2984.745 kb on - strand at 2984.745 kb on - strand at 2984.748 kb on + strand at 2984.748 kb on + strand at 2984.748 kb on + strand at 2984.748 kb on + strand at 2984.748 kb on + strand at 2984.748 kb on + strand at 2984.749 kb on - strand at 2984.749 kb on - strand at 2984.814 kb on + strand at 2984.914 kb on + strand, within RR42_RS13840 at 2984.915 kb on - strand, within RR42_RS13840 at 2985.017 kb on - strand, within RR42_RS13840 at 2985.104 kb on - strand, within RR42_RS13840 at 2985.274 kb on + strand, within RR42_RS13840 at 2985.274 kb on + strand, within RR42_RS13840 at 2985.274 kb on + strand, within RR42_RS13840 at 2985.275 kb on - strand, within RR42_RS13840 at 2985.275 kb on - strand, within RR42_RS13840 at 2985.275 kb on - strand, within RR42_RS13840 at 2985.275 kb on - strand, within RR42_RS13840 at 2985.376 kb on + strand, within RR42_RS13840 at 2985.376 kb on + strand, within RR42_RS13840 at 2985.377 kb on - strand, within RR42_RS13840 at 2985.377 kb on - strand, within RR42_RS13840 at 2985.578 kb on + strand, within RR42_RS13840 at 2985.637 kb on + strand, within RR42_RS13840 at 2985.637 kb on + strand, within RR42_RS13840 at 2985.638 kb on - strand, within RR42_RS13840 at 2985.715 kb on + strand, within RR42_RS13840 at 2985.715 kb on + strand, within RR42_RS13840 at 2985.715 kb on + strand, within RR42_RS13840 at 2985.716 kb on - strand, within RR42_RS13840 at 2985.716 kb on - strand, within RR42_RS13840 at 2985.775 kb on + strand, within RR42_RS13840 at 2985.865 kb on + strand, within RR42_RS13840 at 2985.865 kb on + strand, within RR42_RS13840 at 2985.866 kb on - strand, within RR42_RS13840 at 2985.866 kb on - strand, within RR42_RS13840 at 2985.866 kb on - strand, within RR42_RS13840 at 2985.868 kb on + strand, within RR42_RS13840 at 2985.869 kb on - strand, within RR42_RS13840 at 2985.892 kb on + strand at 2985.892 kb on + strand at 2985.892 kb on + strand at 2985.893 kb on - strand at 2985.893 kb on - strand at 2985.893 kb on - strand at 2985.893 kb on - strand at 2985.961 kb on + strand at 2985.961 kb on + strand at 2985.962 kb on - strand at 2985.962 kb on - strand at 2985.991 kb on + strand at 2986.201 kb on - strand, within RR42_RS13845 at 2986.323 kb on + strand, within RR42_RS13845 at 2986.323 kb on + strand, within RR42_RS13845 at 2986.323 kb on + strand, within RR42_RS13845 at 2986.324 kb on - strand, within RR42_RS13845 at 2986.343 kb on + strand, within RR42_RS13845 at 2986.344 kb on - strand, within RR42_RS13845 at 2986.344 kb on - strand, within RR42_RS13845 at 2986.355 kb on + strand, within RR42_RS13845 at 2986.373 kb on + strand at 2986.374 kb on - strand at 2986.605 kb on + strand, within RR42_RS13850 at 2986.605 kb on + strand, within RR42_RS13850 at 2986.606 kb on - strand, within RR42_RS13850 at 2986.606 kb on - strand, within RR42_RS13850 at 2986.819 kb on + strand at 2986.820 kb on - strand at 2986.820 kb on - strand at 2986.824 kb on - strand at 2986.889 kb on + strand, within RR42_RS13855 at 2986.889 kb on + strand, within RR42_RS13855 at 2986.890 kb on - strand, within RR42_RS13855 at 2986.957 kb on + strand, within RR42_RS13855 at 2986.957 kb on + strand, within RR42_RS13855 at 2986.957 kb on + strand, within RR42_RS13855 at 2986.957 kb on + strand, within RR42_RS13855 at 2986.957 kb on + strand, within RR42_RS13855 at 2986.958 kb on - strand, within RR42_RS13855 at 2986.958 kb on - strand, within RR42_RS13855 at 2986.958 kb on - strand, within RR42_RS13855 at 2986.958 kb on - strand, within RR42_RS13855
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,983,742 + -0.0 2,983,742 + +0.4 2,983,745 - +1.5 2,984,095 + RR42_RS13830 0.69 +1.0 2,984,195 + +0.6 2,984,196 - -0.2 2,984,196 - -1.5 2,984,215 - -1.0 2,984,219 + -0.7 2,984,219 + -1.7 2,984,219 + +0.8 2,984,220 - -1.0 2,984,220 - -2.4 2,984,220 - +1.0 2,984,220 - -1.1 2,984,220 - +0.6 2,984,365 + RR42_RS13835 0.24 +1.2 2,984,366 - RR42_RS13835 0.25 +0.9 2,984,507 - RR42_RS13835 0.86 -0.5 2,984,566 + -1.5 2,984,567 - -1.6 2,984,698 + -2.9 2,984,721 - -0.3 2,984,744 + +3.8 2,984,744 + -0.9 2,984,745 - +0.7 2,984,745 - +0.1 2,984,748 + +0.3 2,984,748 + -0.4 2,984,748 + +0.0 2,984,748 + -1.3 2,984,748 + +0.5 2,984,748 + +1.2 2,984,749 - -0.7 2,984,749 - +0.5 2,984,814 + +0.2 2,984,914 + RR42_RS13840 0.15 +1.4 2,984,915 - RR42_RS13840 0.15 +0.7 2,985,017 - RR42_RS13840 0.23 +1.6 2,985,104 - RR42_RS13840 0.30 -1.8 2,985,274 + RR42_RS13840 0.43 +1.4 2,985,274 + RR42_RS13840 0.43 -1.3 2,985,274 + RR42_RS13840 0.43 +1.3 2,985,275 - RR42_RS13840 0.43 +0.6 2,985,275 - RR42_RS13840 0.43 -0.2 2,985,275 - RR42_RS13840 0.43 +0.2 2,985,275 - RR42_RS13840 0.43 +0.0 2,985,376 + RR42_RS13840 0.51 +0.5 2,985,376 + RR42_RS13840 0.51 -1.0 2,985,377 - RR42_RS13840 0.51 +0.0 2,985,377 - RR42_RS13840 0.51 +0.7 2,985,578 + RR42_RS13840 0.66 +0.8 2,985,637 + RR42_RS13840 0.71 +0.5 2,985,637 + RR42_RS13840 0.71 +0.1 2,985,638 - RR42_RS13840 0.71 -0.2 2,985,715 + RR42_RS13840 0.77 -2.0 2,985,715 + RR42_RS13840 0.77 +0.1 2,985,715 + RR42_RS13840 0.77 -0.3 2,985,716 - RR42_RS13840 0.77 -1.5 2,985,716 - RR42_RS13840 0.77 +1.2 2,985,775 + RR42_RS13840 0.82 -1.8 2,985,865 + RR42_RS13840 0.89 +1.1 2,985,865 + RR42_RS13840 0.89 +1.2 2,985,866 - RR42_RS13840 0.89 +0.5 2,985,866 - RR42_RS13840 0.89 -2.2 2,985,866 - RR42_RS13840 0.89 -0.1 2,985,868 + RR42_RS13840 0.89 +0.0 2,985,869 - RR42_RS13840 0.89 -1.0 2,985,892 + +0.4 2,985,892 + -0.4 2,985,892 + +0.2 2,985,893 - +1.0 2,985,893 - +0.3 2,985,893 - +0.6 2,985,893 - +2.2 2,985,961 + -1.1 2,985,961 + +0.7 2,985,962 - -1.0 2,985,962 - -1.7 2,985,991 + -0.0 2,986,201 - RR42_RS13845 0.31 +0.4 2,986,323 + RR42_RS13845 0.73 +0.4 2,986,323 + RR42_RS13845 0.73 -0.3 2,986,323 + RR42_RS13845 0.73 +1.7 2,986,324 - RR42_RS13845 0.74 +1.7 2,986,343 + RR42_RS13845 0.80 +0.3 2,986,344 - RR42_RS13845 0.81 +0.9 2,986,344 - RR42_RS13845 0.81 +1.7 2,986,355 + RR42_RS13845 0.85 -1.0 2,986,373 + +0.4 2,986,374 - +0.5 2,986,605 + RR42_RS13850 0.40 -1.3 2,986,605 + RR42_RS13850 0.40 -2.0 2,986,606 - RR42_RS13850 0.41 +0.3 2,986,606 - RR42_RS13850 0.41 -0.1 2,986,819 + -0.3 2,986,820 - +1.2 2,986,820 - +0.3 2,986,824 - +0.2 2,986,889 + RR42_RS13855 0.35 -1.0 2,986,889 + RR42_RS13855 0.35 +1.2 2,986,890 - RR42_RS13855 0.35 -0.7 2,986,957 + RR42_RS13855 0.68 +0.0 2,986,957 + RR42_RS13855 0.68 -0.8 2,986,957 + RR42_RS13855 0.68 -0.7 2,986,957 + RR42_RS13855 0.68 -0.5 2,986,957 + RR42_RS13855 0.68 -1.0 2,986,958 - RR42_RS13855 0.69 -2.0 2,986,958 - RR42_RS13855 0.69 +1.2 2,986,958 - RR42_RS13855 0.69 -1.2 2,986,958 - RR42_RS13855 0.69 -0.1
Or see this region's nucleotide sequence