Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13800 and RR42_RS13805 are separated by 29 nucleotides RR42_RS13805 and RR42_RS13810 are separated by 90 nucleotides RR42_RS13810 and RR42_RS13815 are separated by 118 nucleotides
RR42_RS13800: RR42_RS13800 - formyltetrahydrofolate deformylase, at 2,977,512 to 2,978,378
_RS13800
RR42_RS13805: RR42_RS13805 - NTP pyrophosphohydrolase, at 2,978,408 to 2,979,274
_RS13805
RR42_RS13810: RR42_RS13810 - glycerol kinase, at 2,979,365 to 2,980,927
_RS13810
RR42_RS13815: RR42_RS13815 - FAD-dependent oxidoreductase, at 2,981,046 to 2,982,650
_RS13815
Position (kb)
2979
2980
2981 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2978.370 kb on - strand at 2978.507 kb on - strand, within RR42_RS13805 at 2978.598 kb on + strand, within RR42_RS13805 at 2978.598 kb on + strand, within RR42_RS13805 at 2978.599 kb on - strand, within RR42_RS13805 at 2978.599 kb on - strand, within RR42_RS13805 at 2978.604 kb on + strand, within RR42_RS13805 at 2978.604 kb on + strand, within RR42_RS13805 at 2978.604 kb on + strand, within RR42_RS13805 at 2978.604 kb on + strand, within RR42_RS13805 at 2978.604 kb on + strand, within RR42_RS13805 at 2978.605 kb on - strand, within RR42_RS13805 at 2979.100 kb on + strand, within RR42_RS13805 at 2979.101 kb on - strand, within RR42_RS13805 at 2979.326 kb on + strand at 2979.326 kb on + strand at 2979.327 kb on - strand at 2979.331 kb on + strand at 2979.331 kb on + strand at 2979.331 kb on + strand at 2979.332 kb on - strand at 2979.497 kb on + strand at 2979.789 kb on - strand, within RR42_RS13810 at 2979.926 kb on - strand, within RR42_RS13810 at 2980.133 kb on + strand, within RR42_RS13810 at 2980.134 kb on - strand, within RR42_RS13810 at 2980.134 kb on - strand, within RR42_RS13810 at 2980.171 kb on + strand, within RR42_RS13810 at 2980.171 kb on + strand, within RR42_RS13810 at 2980.172 kb on - strand, within RR42_RS13810 at 2980.175 kb on + strand, within RR42_RS13810 at 2980.176 kb on - strand, within RR42_RS13810 at 2980.176 kb on - strand, within RR42_RS13810 at 2980.298 kb on + strand, within RR42_RS13810 at 2980.870 kb on - strand at 2980.967 kb on + strand at 2980.967 kb on + strand at 2980.967 kb on + strand at 2980.968 kb on - strand at 2980.968 kb on - strand at 2980.975 kb on + strand at 2980.975 kb on + strand at 2980.975 kb on + strand at 2980.976 kb on - strand at 2980.983 kb on + strand at 2980.983 kb on + strand at 2980.991 kb on + strand at 2980.992 kb on - strand at 2980.999 kb on + strand at 2980.999 kb on + strand at 2980.999 kb on + strand at 2980.999 kb on + strand at 2980.999 kb on + strand at 2980.999 kb on + strand at 2980.999 kb on + strand at 2980.999 kb on + strand at 2981.003 kb on + strand at 2981.003 kb on + strand at 2981.003 kb on + strand at 2981.641 kb on - strand, within RR42_RS13815
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,978,370 - -0.8 2,978,507 - RR42_RS13805 0.11 +0.0 2,978,598 + RR42_RS13805 0.22 +0.5 2,978,598 + RR42_RS13805 0.22 +1.4 2,978,599 - RR42_RS13805 0.22 -0.8 2,978,599 - RR42_RS13805 0.22 -0.1 2,978,604 + RR42_RS13805 0.23 +2.0 2,978,604 + RR42_RS13805 0.23 +1.5 2,978,604 + RR42_RS13805 0.23 +1.6 2,978,604 + RR42_RS13805 0.23 -0.1 2,978,604 + RR42_RS13805 0.23 -1.1 2,978,605 - RR42_RS13805 0.23 -0.6 2,979,100 + RR42_RS13805 0.80 +0.0 2,979,101 - RR42_RS13805 0.80 -0.3 2,979,326 + -0.8 2,979,326 + -0.4 2,979,327 - -0.4 2,979,331 + -0.8 2,979,331 + -0.3 2,979,331 + -0.6 2,979,332 - +0.0 2,979,497 + -0.1 2,979,789 - RR42_RS13810 0.27 -2.0 2,979,926 - RR42_RS13810 0.36 +1.0 2,980,133 + RR42_RS13810 0.49 -2.4 2,980,134 - RR42_RS13810 0.49 -0.2 2,980,134 - RR42_RS13810 0.49 -2.9 2,980,171 + RR42_RS13810 0.52 -0.2 2,980,171 + RR42_RS13810 0.52 -0.6 2,980,172 - RR42_RS13810 0.52 -3.4 2,980,175 + RR42_RS13810 0.52 +1.3 2,980,176 - RR42_RS13810 0.52 -0.6 2,980,176 - RR42_RS13810 0.52 -0.0 2,980,298 + RR42_RS13810 0.60 +0.4 2,980,870 - -0.6 2,980,967 + +1.0 2,980,967 + +1.0 2,980,967 + -0.6 2,980,968 - -0.3 2,980,968 - -0.6 2,980,975 + -0.6 2,980,975 + +0.3 2,980,975 + +0.4 2,980,976 - +1.0 2,980,983 + +0.4 2,980,983 + +0.6 2,980,991 + -0.2 2,980,992 - -1.3 2,980,999 + -0.2 2,980,999 + +0.7 2,980,999 + +1.4 2,980,999 + -0.6 2,980,999 + +0.8 2,980,999 + -0.4 2,980,999 + +0.9 2,980,999 + +0.3 2,981,003 + -0.7 2,981,003 + +0.3 2,981,003 + -0.3 2,981,641 - RR42_RS13815 0.37 -0.8
Or see this region's nucleotide sequence