Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13675 and RR42_RS13680 are separated by 5 nucleotides RR42_RS13680 and RR42_RS13685 are separated by 68 nucleotides RR42_RS13685 and RR42_RS13690 are separated by 16 nucleotides RR42_RS13690 and RR42_RS13695 overlap by 8 nucleotides
RR42_RS13675: RR42_RS13675 - short-chain dehydrogenase, at 2,952,805 to 2,953,608
_RS13675
RR42_RS13680: RR42_RS13680 - IclR family transcriptional regulator, at 2,953,614 to 2,954,444
_RS13680
RR42_RS13685: RR42_RS13685 - cupin, at 2,954,513 to 2,955,049
_RS13685
RR42_RS13690: RR42_RS13690 - short-chain dehydrogenase, at 2,955,066 to 2,955,836
_RS13690
RR42_RS13695: RR42_RS13695 - 3-phenylpropionate dioxygenase, at 2,955,829 to 2,956,884
_RS13695
Position (kb)
2954
2955
2956 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2953.528 kb on + strand at 2953.625 kb on - strand at 2953.784 kb on + strand, within RR42_RS13680 at 2953.784 kb on + strand, within RR42_RS13680 at 2953.785 kb on - strand, within RR42_RS13680 at 2953.785 kb on - strand, within RR42_RS13680 at 2954.074 kb on + strand, within RR42_RS13680 at 2954.075 kb on - strand, within RR42_RS13680 at 2954.185 kb on + strand, within RR42_RS13680 at 2954.404 kb on + strand at 2954.405 kb on - strand at 2954.405 kb on - strand at 2954.405 kb on - strand at 2954.872 kb on - strand, within RR42_RS13685 at 2954.893 kb on - strand, within RR42_RS13685 at 2954.904 kb on + strand, within RR42_RS13685 at 2955.062 kb on + strand at 2955.157 kb on - strand, within RR42_RS13690 at 2955.157 kb on - strand, within RR42_RS13690 at 2955.203 kb on - strand, within RR42_RS13690 at 2955.229 kb on + strand, within RR42_RS13690 at 2955.230 kb on - strand, within RR42_RS13690 at 2955.230 kb on - strand, within RR42_RS13690 at 2955.349 kb on + strand, within RR42_RS13690 at 2955.476 kb on + strand, within RR42_RS13690 at 2955.476 kb on + strand, within RR42_RS13690
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,953,528 + -0.3 2,953,625 - +0.1 2,953,784 + RR42_RS13680 0.20 +0.2 2,953,784 + RR42_RS13680 0.20 +2.3 2,953,785 - RR42_RS13680 0.21 +0.8 2,953,785 - RR42_RS13680 0.21 -0.7 2,954,074 + RR42_RS13680 0.55 -0.8 2,954,075 - RR42_RS13680 0.55 -3.3 2,954,185 + RR42_RS13680 0.69 +0.4 2,954,404 + -2.1 2,954,405 - -0.6 2,954,405 - +0.8 2,954,405 - +0.6 2,954,872 - RR42_RS13685 0.67 -2.3 2,954,893 - RR42_RS13685 0.71 -3.9 2,954,904 + RR42_RS13685 0.73 +0.2 2,955,062 + +0.5 2,955,157 - RR42_RS13690 0.12 -0.0 2,955,157 - RR42_RS13690 0.12 +0.5 2,955,203 - RR42_RS13690 0.18 +0.3 2,955,229 + RR42_RS13690 0.21 -0.8 2,955,230 - RR42_RS13690 0.21 +0.3 2,955,230 - RR42_RS13690 0.21 -0.3 2,955,349 + RR42_RS13690 0.37 -0.1 2,955,476 + RR42_RS13690 0.53 +0.8 2,955,476 + RR42_RS13690 0.53 -0.6
Or see this region's nucleotide sequence