Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13610 and RR42_RS13615 are separated by 184 nucleotides RR42_RS13615 and RR42_RS13620 overlap by 11 nucleotides RR42_RS13620 and RR42_RS13625 are separated by 9 nucleotides RR42_RS13625 and RR42_RS13630 overlap by 8 nucleotides
RR42_RS13610: RR42_RS13610 - IclR family transcriptional regulator, at 2,937,579 to 2,938,400
_RS13610
RR42_RS13615: RR42_RS13615 - 4-hydroxybenzoyl-CoA reductase, at 2,938,585 to 2,939,370
_RS13615
RR42_RS13620: RR42_RS13620 - ferredoxin, at 2,939,360 to 2,939,836
_RS13620
RR42_RS13625: RR42_RS13625 - 4-hydroxybenzoyl-CoA reductase, at 2,939,846 to 2,940,712
_RS13625
RR42_RS13630: RR42_RS13630 - 4-hydroxybenzoyl-CoA reductase, at 2,940,705 to 2,942,999
_RS13630
Position (kb)
2939
2940 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2938.360 kb on + strand at 2938.360 kb on + strand at 2938.360 kb on + strand at 2938.360 kb on + strand at 2938.361 kb on - strand at 2938.361 kb on - strand at 2938.361 kb on - strand at 2938.361 kb on - strand at 2938.380 kb on - strand at 2938.406 kb on + strand at 2938.406 kb on + strand at 2938.407 kb on - strand at 2938.423 kb on - strand at 2938.438 kb on - strand at 2938.438 kb on - strand at 2938.530 kb on + strand at 2938.530 kb on + strand at 2938.531 kb on - strand at 2938.532 kb on + strand at 2938.532 kb on + strand at 2938.533 kb on - strand at 2938.548 kb on + strand at 2938.548 kb on + strand at 2938.595 kb on + strand at 2938.596 kb on - strand at 2938.596 kb on - strand at 2938.686 kb on + strand, within RR42_RS13615 at 2938.687 kb on - strand, within RR42_RS13615 at 2938.687 kb on - strand, within RR42_RS13615 at 2938.771 kb on + strand, within RR42_RS13615 at 2938.772 kb on - strand, within RR42_RS13615 at 2939.145 kb on + strand, within RR42_RS13615 at 2939.153 kb on + strand, within RR42_RS13615 at 2939.153 kb on + strand, within RR42_RS13615 at 2939.154 kb on - strand at 2939.154 kb on - strand, within RR42_RS13615 at 2939.154 kb on - strand, within RR42_RS13615 at 2939.240 kb on + strand, within RR42_RS13615 at 2939.240 kb on + strand, within RR42_RS13615 at 2939.241 kb on - strand, within RR42_RS13615 at 2939.241 kb on - strand, within RR42_RS13615 at 2939.241 kb on - strand, within RR42_RS13615 at 2939.529 kb on + strand, within RR42_RS13620 at 2939.847 kb on + strand at 2939.916 kb on + strand at 2940.092 kb on - strand, within RR42_RS13625 at 2940.168 kb on + strand, within RR42_RS13625 at 2940.246 kb on + strand, within RR42_RS13625 at 2940.247 kb on - strand, within RR42_RS13625 at 2940.363 kb on + strand, within RR42_RS13625 at 2940.707 kb on - strand at 2940.805 kb on + strand at 2940.805 kb on + strand at 2940.806 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,938,360 + -0.2 2,938,360 + -0.9 2,938,360 + -0.0 2,938,360 + +0.9 2,938,361 - -0.8 2,938,361 - -1.6 2,938,361 - +1.0 2,938,361 - +0.9 2,938,380 - +0.2 2,938,406 + +1.1 2,938,406 + -1.3 2,938,407 - +0.2 2,938,423 - +3.0 2,938,438 - +0.7 2,938,438 - +0.1 2,938,530 + -1.6 2,938,530 + +0.3 2,938,531 - +0.7 2,938,532 + -1.3 2,938,532 + +0.4 2,938,533 - +1.2 2,938,548 + +2.1 2,938,548 + -0.9 2,938,595 + -0.8 2,938,596 - +1.0 2,938,596 - -1.8 2,938,686 + RR42_RS13615 0.13 +1.4 2,938,687 - RR42_RS13615 0.13 -0.4 2,938,687 - RR42_RS13615 0.13 +0.6 2,938,771 + RR42_RS13615 0.24 +0.2 2,938,772 - RR42_RS13615 0.24 +0.3 2,939,145 + RR42_RS13615 0.71 -2.1 2,939,153 + RR42_RS13615 0.72 -0.8 2,939,153 + RR42_RS13615 0.72 +0.4 2,939,154 - -0.0 2,939,154 - RR42_RS13615 0.72 -1.3 2,939,154 - RR42_RS13615 0.72 -0.6 2,939,240 + RR42_RS13615 0.83 -3.3 2,939,240 + RR42_RS13615 0.83 -1.6 2,939,241 - RR42_RS13615 0.83 +0.0 2,939,241 - RR42_RS13615 0.83 +0.6 2,939,241 - RR42_RS13615 0.83 -0.6 2,939,529 + RR42_RS13620 0.35 -1.0 2,939,847 + -0.6 2,939,916 + -1.0 2,940,092 - RR42_RS13625 0.28 +0.6 2,940,168 + RR42_RS13625 0.37 +1.6 2,940,246 + RR42_RS13625 0.46 -0.9 2,940,247 - RR42_RS13625 0.46 -1.1 2,940,363 + RR42_RS13625 0.60 +0.3 2,940,707 - -1.8 2,940,805 + -0.5 2,940,805 + -1.2 2,940,806 - +1.3
Or see this region's nucleotide sequence