Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12985 and RR42_RS12990 are separated by 4 nucleotides RR42_RS12990 and RR42_RS12995 are separated by 133 nucleotides RR42_RS12995 and RR42_RS13000 are separated by 572 nucleotides
RR42_RS12985: RR42_RS12985 - histidine kinase, at 2,795,106 to 2,796,257
_RS12985
RR42_RS12990: RR42_RS12990 - hypothetical protein, at 2,796,262 to 2,796,825
_RS12990
RR42_RS12995: RR42_RS12995 - glutamine synthetase, at 2,796,959 to 2,798,374
_RS12995
RR42_RS13000: RR42_RS13000 - sulfurtransferase, at 2,798,947 to 2,799,411
_RS13000
Position (kb)
2796
2797
2798
2799 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2796.086 kb on - strand, within RR42_RS12985 at 2796.154 kb on + strand at 2796.154 kb on + strand at 2796.155 kb on - strand at 2796.257 kb on + strand at 2796.263 kb on + strand at 2796.264 kb on - strand at 2796.264 kb on - strand at 2796.356 kb on + strand, within RR42_RS12990 at 2796.357 kb on - strand, within RR42_RS12990 at 2796.357 kb on - strand, within RR42_RS12990 at 2796.357 kb on - strand, within RR42_RS12990 at 2796.365 kb on + strand, within RR42_RS12990 at 2796.366 kb on - strand, within RR42_RS12990 at 2796.369 kb on + strand, within RR42_RS12990 at 2796.370 kb on - strand, within RR42_RS12990 at 2796.680 kb on + strand, within RR42_RS12990 at 2796.680 kb on + strand, within RR42_RS12990 at 2796.680 kb on + strand, within RR42_RS12990 at 2796.681 kb on - strand, within RR42_RS12990 at 2796.681 kb on - strand, within RR42_RS12990 at 2796.864 kb on - strand at 2796.864 kb on - strand at 2798.464 kb on - strand at 2798.526 kb on - strand at 2798.530 kb on - strand at 2798.602 kb on + strand at 2798.609 kb on + strand at 2798.610 kb on - strand at 2798.610 kb on - strand at 2798.610 kb on - strand at 2798.615 kb on + strand at 2798.615 kb on + strand at 2798.616 kb on - strand at 2798.714 kb on + strand at 2798.726 kb on + strand at 2798.890 kb on + strand at 2798.890 kb on + strand at 2798.967 kb on - strand at 2798.967 kb on - strand at 2799.010 kb on + strand, within RR42_RS13000 at 2799.010 kb on + strand, within RR42_RS13000 at 2799.010 kb on + strand, within RR42_RS13000 at 2799.011 kb on - strand, within RR42_RS13000 at 2799.163 kb on + strand, within RR42_RS13000 at 2799.164 kb on - strand, within RR42_RS13000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,796,086 - RR42_RS12985 0.85 -0.6 2,796,154 + -2.1 2,796,154 + -0.5 2,796,155 - +0.2 2,796,257 + +3.9 2,796,263 + -0.0 2,796,264 - -0.2 2,796,264 - -1.4 2,796,356 + RR42_RS12990 0.17 +0.9 2,796,357 - RR42_RS12990 0.17 -1.1 2,796,357 - RR42_RS12990 0.17 -0.5 2,796,357 - RR42_RS12990 0.17 +1.9 2,796,365 + RR42_RS12990 0.18 -0.6 2,796,366 - RR42_RS12990 0.18 -1.0 2,796,369 + RR42_RS12990 0.19 +0.2 2,796,370 - RR42_RS12990 0.19 +0.0 2,796,680 + RR42_RS12990 0.74 -2.4 2,796,680 + RR42_RS12990 0.74 -2.1 2,796,680 + RR42_RS12990 0.74 +0.8 2,796,681 - RR42_RS12990 0.74 -0.3 2,796,681 - RR42_RS12990 0.74 +1.9 2,796,864 - -1.4 2,796,864 - -0.5 2,798,464 - -0.6 2,798,526 - -1.4 2,798,530 - -0.1 2,798,602 + +0.1 2,798,609 + +0.7 2,798,610 - +0.9 2,798,610 - -0.8 2,798,610 - +0.9 2,798,615 + -0.2 2,798,615 + -0.8 2,798,616 - +1.6 2,798,714 + +0.6 2,798,726 + +1.2 2,798,890 + -0.1 2,798,890 + +0.3 2,798,967 - -1.7 2,798,967 - -0.6 2,799,010 + RR42_RS13000 0.14 +0.4 2,799,010 + RR42_RS13000 0.14 +0.4 2,799,010 + RR42_RS13000 0.14 -1.2 2,799,011 - RR42_RS13000 0.14 -0.4 2,799,163 + RR42_RS13000 0.46 -1.9 2,799,164 - RR42_RS13000 0.47 -0.2
Or see this region's nucleotide sequence