Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12955 and RR42_RS12960 are separated by 68 nucleotides RR42_RS12960 and RR42_RS12965 are separated by 12 nucleotides RR42_RS12965 and RR42_RS12970 are separated by 124 nucleotides
RR42_RS12955: RR42_RS12955 - glycerol-3-phosphate ABC transporter ATP-binding protein, at 2,788,036 to 2,789,139
_RS12955
RR42_RS12960: RR42_RS12960 - glycerol-3-phosphate transporter membrane protein, at 2,789,208 to 2,790,056
_RS12960
RR42_RS12965: RR42_RS12965 - glycerol-3-phosphate transporter permease, at 2,790,069 to 2,790,950
_RS12965
RR42_RS12970: RR42_RS12970 - glycerol-3-phosphate ABC transporter substrate-binding protein, at 2,791,075 to 2,792,391
_RS12970
Position (kb)
2790
2791 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2789.102 kb on + strand at 2789.103 kb on - strand at 2789.103 kb on - strand at 2789.114 kb on - strand at 2789.143 kb on + strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.413 kb on + strand, within RR42_RS12960 at 2789.429 kb on - strand, within RR42_RS12960 at 2789.429 kb on - strand, within RR42_RS12960 at 2789.623 kb on + strand, within RR42_RS12960 at 2789.659 kb on + strand, within RR42_RS12960 at 2789.659 kb on + strand, within RR42_RS12960 at 2789.747 kb on - strand, within RR42_RS12960 at 2789.747 kb on - strand, within RR42_RS12960 at 2789.884 kb on + strand, within RR42_RS12960 at 2789.884 kb on + strand, within RR42_RS12960 at 2789.974 kb on + strand at 2789.974 kb on + strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2790.074 kb on - strand at 2790.194 kb on - strand, within RR42_RS12965 at 2790.202 kb on + strand, within RR42_RS12965 at 2790.202 kb on + strand, within RR42_RS12965 at 2790.202 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.213 kb on - strand, within RR42_RS12965 at 2790.280 kb on + strand, within RR42_RS12965 at 2790.280 kb on + strand, within RR42_RS12965 at 2790.281 kb on - strand, within RR42_RS12965 at 2790.281 kb on - strand, within RR42_RS12965 at 2790.281 kb on - strand, within RR42_RS12965 at 2790.333 kb on + strand, within RR42_RS12965 at 2790.398 kb on + strand, within RR42_RS12965 at 2790.399 kb on - strand, within RR42_RS12965 at 2790.439 kb on + strand, within RR42_RS12965 at 2790.440 kb on - strand, within RR42_RS12965 at 2790.538 kb on + strand, within RR42_RS12965 at 2790.611 kb on - strand, within RR42_RS12965 at 2790.670 kb on + strand, within RR42_RS12965 at 2790.670 kb on + strand, within RR42_RS12965 at 2790.751 kb on + strand, within RR42_RS12965 at 2790.842 kb on - strand, within RR42_RS12965 at 2790.842 kb on - strand, within RR42_RS12965 at 2790.910 kb on + strand at 2790.911 kb on - strand at 2790.993 kb on + strand at 2790.993 kb on + strand at 2790.993 kb on + strand at 2790.994 kb on - strand at 2790.994 kb on - strand at 2790.997 kb on + strand at 2790.997 kb on + strand at 2790.998 kb on - strand at 2790.998 kb on - strand at 2791.077 kb on - strand at 2791.149 kb on + strand at 2791.149 kb on + strand at 2791.150 kb on - strand at 2791.150 kb on - strand at 2791.150 kb on - strand at 2791.200 kb on + strand at 2791.302 kb on - strand, within RR42_RS12970 at 2791.302 kb on - strand, within RR42_RS12970 at 2791.328 kb on + strand, within RR42_RS12970 at 2791.328 kb on + strand, within RR42_RS12970 at 2791.400 kb on + strand at 2791.400 kb on + strand, within RR42_RS12970 at 2791.400 kb on + strand, within RR42_RS12970 at 2791.427 kb on + strand, within RR42_RS12970 at 2791.427 kb on + strand, within RR42_RS12970 at 2791.428 kb on - strand, within RR42_RS12970 at 2791.428 kb on - strand, within RR42_RS12970 at 2791.514 kb on + strand, within RR42_RS12970 at 2791.515 kb on - strand, within RR42_RS12970 at 2791.612 kb on - strand, within RR42_RS12970 at 2791.612 kb on - strand, within RR42_RS12970 at 2791.647 kb on - strand, within RR42_RS12970 at 2791.655 kb on + strand, within RR42_RS12970 at 2791.689 kb on + strand, within RR42_RS12970 at 2791.689 kb on + strand, within RR42_RS12970 at 2791.689 kb on + strand, within RR42_RS12970 at 2791.689 kb on + strand, within RR42_RS12970 at 2791.827 kb on - strand, within RR42_RS12970 at 2791.940 kb on + strand, within RR42_RS12970 at 2791.940 kb on + strand, within RR42_RS12970 at 2791.940 kb on + strand, within RR42_RS12970 at 2791.940 kb on + strand, within RR42_RS12970 at 2791.941 kb on - strand, within RR42_RS12970 at 2791.943 kb on + strand, within RR42_RS12970 at 2791.943 kb on + strand, within RR42_RS12970 at 2791.943 kb on + strand, within RR42_RS12970 at 2791.943 kb on + strand, within RR42_RS12970 at 2791.944 kb on - strand, within RR42_RS12970 at 2791.944 kb on - strand, within RR42_RS12970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,789,102 + +0.6 2,789,103 - -2.3 2,789,103 - +0.2 2,789,114 - -3.2 2,789,143 + -2.9 2,789,144 - -3.1 2,789,144 - -0.4 2,789,144 - -2.3 2,789,144 - -2.8 2,789,144 - -2.6 2,789,413 + RR42_RS12960 0.24 -2.1 2,789,429 - RR42_RS12960 0.26 +0.5 2,789,429 - RR42_RS12960 0.26 +0.2 2,789,623 + RR42_RS12960 0.49 +0.9 2,789,659 + RR42_RS12960 0.53 -3.8 2,789,659 + RR42_RS12960 0.53 -2.0 2,789,747 - RR42_RS12960 0.63 -2.2 2,789,747 - RR42_RS12960 0.63 -1.4 2,789,884 + RR42_RS12960 0.80 +0.8 2,789,884 + RR42_RS12960 0.80 -1.6 2,789,974 + -1.4 2,789,974 + -1.3 2,789,975 - -2.6 2,789,975 - -0.9 2,789,975 - -1.1 2,789,975 - -1.1 2,789,975 - -0.5 2,789,975 - -0.7 2,790,074 - -1.1 2,790,194 - RR42_RS12965 0.14 -1.0 2,790,202 + RR42_RS12965 0.15 +0.1 2,790,202 + RR42_RS12965 0.15 -1.2 2,790,202 + RR42_RS12965 0.15 +0.4 2,790,212 + RR42_RS12965 0.16 -2.5 2,790,212 + RR42_RS12965 0.16 -0.0 2,790,212 + RR42_RS12965 0.16 -0.2 2,790,212 + RR42_RS12965 0.16 -1.2 2,790,212 + RR42_RS12965 0.16 -0.9 2,790,213 - RR42_RS12965 0.16 -3.6 2,790,280 + RR42_RS12965 0.24 -2.0 2,790,280 + RR42_RS12965 0.24 -3.1 2,790,281 - RR42_RS12965 0.24 +1.1 2,790,281 - RR42_RS12965 0.24 +0.4 2,790,281 - RR42_RS12965 0.24 -0.9 2,790,333 + RR42_RS12965 0.30 -0.3 2,790,398 + RR42_RS12965 0.37 +0.6 2,790,399 - RR42_RS12965 0.37 +0.1 2,790,439 + RR42_RS12965 0.42 +1.8 2,790,440 - RR42_RS12965 0.42 -0.4 2,790,538 + RR42_RS12965 0.53 +0.8 2,790,611 - RR42_RS12965 0.61 -0.0 2,790,670 + RR42_RS12965 0.68 +0.2 2,790,670 + RR42_RS12965 0.68 +2.5 2,790,751 + RR42_RS12965 0.77 -2.9 2,790,842 - RR42_RS12965 0.88 +0.5 2,790,842 - RR42_RS12965 0.88 -0.3 2,790,910 + -0.0 2,790,911 - +0.6 2,790,993 + -2.8 2,790,993 + -0.5 2,790,993 + -1.4 2,790,994 - +1.9 2,790,994 - +0.2 2,790,997 + -1.1 2,790,997 + -0.1 2,790,998 - -0.3 2,790,998 - -0.1 2,791,077 - +0.2 2,791,149 + -1.2 2,791,149 + -0.6 2,791,150 - -1.4 2,791,150 - +0.3 2,791,150 - -0.1 2,791,200 + -2.6 2,791,302 - RR42_RS12970 0.17 +1.1 2,791,302 - RR42_RS12970 0.17 -0.3 2,791,328 + RR42_RS12970 0.19 -0.3 2,791,328 + RR42_RS12970 0.19 -1.4 2,791,400 + +0.6 2,791,400 + RR42_RS12970 0.25 +1.7 2,791,400 + RR42_RS12970 0.25 -0.4 2,791,427 + RR42_RS12970 0.27 -1.4 2,791,427 + RR42_RS12970 0.27 -1.1 2,791,428 - RR42_RS12970 0.27 -0.8 2,791,428 - RR42_RS12970 0.27 -0.1 2,791,514 + RR42_RS12970 0.33 -2.6 2,791,515 - RR42_RS12970 0.33 -1.9 2,791,612 - RR42_RS12970 0.41 -1.0 2,791,612 - RR42_RS12970 0.41 -0.1 2,791,647 - RR42_RS12970 0.43 -0.1 2,791,655 + RR42_RS12970 0.44 -1.2 2,791,689 + RR42_RS12970 0.47 +1.0 2,791,689 + RR42_RS12970 0.47 +1.5 2,791,689 + RR42_RS12970 0.47 -1.2 2,791,689 + RR42_RS12970 0.47 +0.9 2,791,827 - RR42_RS12970 0.57 -2.1 2,791,940 + RR42_RS12970 0.66 +0.1 2,791,940 + RR42_RS12970 0.66 -0.9 2,791,940 + RR42_RS12970 0.66 -2.8 2,791,940 + RR42_RS12970 0.66 +1.2 2,791,941 - RR42_RS12970 0.66 -0.1 2,791,943 + RR42_RS12970 0.66 -1.4 2,791,943 + RR42_RS12970 0.66 -3.2 2,791,943 + RR42_RS12970 0.66 -1.8 2,791,943 + RR42_RS12970 0.66 -0.5 2,791,944 - RR42_RS12970 0.66 +0.9 2,791,944 - RR42_RS12970 0.66 -0.3
Or see this region's nucleotide sequence