Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12945 and RR42_RS12950 are separated by 28 nucleotides RR42_RS12950 and RR42_RS12955 are separated by 1 nucleotides RR42_RS12955 and RR42_RS12960 are separated by 68 nucleotides RR42_RS12960 and RR42_RS12965 are separated by 12 nucleotides
RR42_RS12945: RR42_RS12945 - membrane protein, at 2,785,664 to 2,787,259
_RS12945
RR42_RS12950: RR42_RS12950 - membrane protein, at 2,787,288 to 2,788,034
_RS12950
RR42_RS12955: RR42_RS12955 - glycerol-3-phosphate ABC transporter ATP-binding protein, at 2,788,036 to 2,789,139
_RS12955
RR42_RS12960: RR42_RS12960 - glycerol-3-phosphate transporter membrane protein, at 2,789,208 to 2,790,056
_RS12960
RR42_RS12965: RR42_RS12965 - glycerol-3-phosphate transporter permease, at 2,790,069 to 2,790,950
_RS12965
Position (kb)
2788
2789
2790 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2787.120 kb on + strand at 2787.201 kb on + strand at 2787.202 kb on - strand at 2787.290 kb on - strand at 2787.386 kb on + strand, within RR42_RS12950 at 2787.387 kb on - strand, within RR42_RS12950 at 2787.388 kb on + strand, within RR42_RS12950 at 2787.388 kb on + strand, within RR42_RS12950 at 2787.720 kb on - strand, within RR42_RS12950 at 2787.721 kb on + strand, within RR42_RS12950 at 2787.721 kb on + strand, within RR42_RS12950 at 2787.722 kb on - strand, within RR42_RS12950 at 2787.722 kb on - strand, within RR42_RS12950 at 2788.012 kb on + strand at 2788.013 kb on - strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.132 kb on - strand at 2788.132 kb on - strand at 2788.132 kb on - strand at 2788.132 kb on - strand at 2788.132 kb on - strand at 2788.178 kb on + strand, within RR42_RS12955 at 2788.179 kb on - strand, within RR42_RS12955 at 2788.179 kb on - strand, within RR42_RS12955 at 2788.185 kb on - strand, within RR42_RS12955 at 2788.261 kb on - strand, within RR42_RS12955 at 2788.472 kb on + strand, within RR42_RS12955 at 2788.472 kb on + strand, within RR42_RS12955 at 2788.472 kb on + strand, within RR42_RS12955 at 2788.473 kb on - strand, within RR42_RS12955 at 2788.473 kb on - strand, within RR42_RS12955 at 2788.473 kb on - strand, within RR42_RS12955 at 2788.571 kb on + strand, within RR42_RS12955 at 2788.571 kb on + strand, within RR42_RS12955 at 2788.571 kb on + strand at 2788.571 kb on + strand, within RR42_RS12955 at 2788.571 kb on + strand, within RR42_RS12955 at 2788.571 kb on + strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.691 kb on - strand, within RR42_RS12955 at 2788.691 kb on - strand, within RR42_RS12955 at 2788.851 kb on + strand, within RR42_RS12955 at 2788.851 kb on + strand, within RR42_RS12955 at 2788.852 kb on - strand, within RR42_RS12955 at 2788.869 kb on + strand, within RR42_RS12955 at 2788.869 kb on + strand, within RR42_RS12955 at 2788.878 kb on - strand, within RR42_RS12955 at 2788.953 kb on + strand, within RR42_RS12955 at 2788.953 kb on + strand, within RR42_RS12955 at 2788.954 kb on - strand, within RR42_RS12955 at 2788.954 kb on - strand, within RR42_RS12955 at 2788.954 kb on - strand, within RR42_RS12955 at 2789.102 kb on + strand at 2789.103 kb on - strand at 2789.103 kb on - strand at 2789.114 kb on - strand at 2789.143 kb on + strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.413 kb on + strand, within RR42_RS12960 at 2789.429 kb on - strand, within RR42_RS12960 at 2789.429 kb on - strand, within RR42_RS12960 at 2789.623 kb on + strand, within RR42_RS12960 at 2789.659 kb on + strand, within RR42_RS12960 at 2789.659 kb on + strand, within RR42_RS12960 at 2789.747 kb on - strand, within RR42_RS12960 at 2789.747 kb on - strand, within RR42_RS12960 at 2789.884 kb on + strand, within RR42_RS12960 at 2789.884 kb on + strand, within RR42_RS12960 at 2789.974 kb on + strand at 2789.974 kb on + strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2790.074 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,787,120 + -0.1 2,787,201 + -0.6 2,787,202 - +0.3 2,787,290 - -2.1 2,787,386 + RR42_RS12950 0.13 -1.2 2,787,387 - RR42_RS12950 0.13 -0.3 2,787,388 + RR42_RS12950 0.13 +1.0 2,787,388 + RR42_RS12950 0.13 -1.9 2,787,720 - RR42_RS12950 0.58 -0.4 2,787,721 + RR42_RS12950 0.58 -0.3 2,787,721 + RR42_RS12950 0.58 -0.8 2,787,722 - RR42_RS12950 0.58 +0.9 2,787,722 - RR42_RS12950 0.58 +0.5 2,788,012 + +0.4 2,788,013 - +1.4 2,788,131 + -1.0 2,788,131 + -3.2 2,788,131 + -0.2 2,788,131 + -0.1 2,788,131 + -0.9 2,788,131 + -2.1 2,788,132 - +1.6 2,788,132 - -1.4 2,788,132 - -0.1 2,788,132 - -0.0 2,788,132 - -2.0 2,788,178 + RR42_RS12955 0.13 -1.0 2,788,179 - RR42_RS12955 0.13 +0.1 2,788,179 - RR42_RS12955 0.13 -0.6 2,788,185 - RR42_RS12955 0.13 +0.1 2,788,261 - RR42_RS12955 0.20 +0.2 2,788,472 + RR42_RS12955 0.39 +0.6 2,788,472 + RR42_RS12955 0.39 +0.0 2,788,472 + RR42_RS12955 0.39 +1.1 2,788,473 - RR42_RS12955 0.40 -0.2 2,788,473 - RR42_RS12955 0.40 -1.4 2,788,473 - RR42_RS12955 0.40 +0.3 2,788,571 + RR42_RS12955 0.48 -0.5 2,788,571 + RR42_RS12955 0.48 +0.6 2,788,571 + -0.1 2,788,571 + RR42_RS12955 0.48 -1.0 2,788,571 + RR42_RS12955 0.48 -1.5 2,788,571 + RR42_RS12955 0.48 -1.4 2,788,572 - RR42_RS12955 0.49 -0.1 2,788,572 - RR42_RS12955 0.49 +0.0 2,788,572 - RR42_RS12955 0.49 -2.2 2,788,572 - RR42_RS12955 0.49 -1.2 2,788,572 - RR42_RS12955 0.49 +0.9 2,788,572 - RR42_RS12955 0.49 -2.6 2,788,572 - RR42_RS12955 0.49 -0.6 2,788,572 - RR42_RS12955 0.49 +0.0 2,788,572 - RR42_RS12955 0.49 -2.6 2,788,572 - RR42_RS12955 0.49 -1.8 2,788,572 - RR42_RS12955 0.49 -1.1 2,788,691 - RR42_RS12955 0.59 +0.7 2,788,691 - RR42_RS12955 0.59 -0.9 2,788,851 + RR42_RS12955 0.74 -1.6 2,788,851 + RR42_RS12955 0.74 -0.4 2,788,852 - RR42_RS12955 0.74 -0.4 2,788,869 + RR42_RS12955 0.75 +0.5 2,788,869 + RR42_RS12955 0.75 -0.1 2,788,878 - RR42_RS12955 0.76 +0.4 2,788,953 + RR42_RS12955 0.83 +0.7 2,788,953 + RR42_RS12955 0.83 -2.1 2,788,954 - RR42_RS12955 0.83 -3.9 2,788,954 - RR42_RS12955 0.83 -2.4 2,788,954 - RR42_RS12955 0.83 -1.2 2,789,102 + +0.6 2,789,103 - -2.3 2,789,103 - +0.2 2,789,114 - -3.2 2,789,143 + -2.9 2,789,144 - -3.1 2,789,144 - -0.4 2,789,144 - -2.3 2,789,144 - -2.8 2,789,144 - -2.6 2,789,413 + RR42_RS12960 0.24 -2.1 2,789,429 - RR42_RS12960 0.26 +0.5 2,789,429 - RR42_RS12960 0.26 +0.2 2,789,623 + RR42_RS12960 0.49 +0.9 2,789,659 + RR42_RS12960 0.53 -3.8 2,789,659 + RR42_RS12960 0.53 -2.0 2,789,747 - RR42_RS12960 0.63 -2.2 2,789,747 - RR42_RS12960 0.63 -1.4 2,789,884 + RR42_RS12960 0.80 +0.8 2,789,884 + RR42_RS12960 0.80 -1.6 2,789,974 + -1.4 2,789,974 + -1.3 2,789,975 - -2.6 2,789,975 - -0.9 2,789,975 - -1.1 2,789,975 - -1.1 2,789,975 - -0.5 2,789,975 - -0.7 2,790,074 - -1.1
Or see this region's nucleotide sequence