Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12690 and RR42_RS12695 are separated by 557 nucleotides RR42_RS12695 and RR42_RS12700 are separated by 115 nucleotides RR42_RS12700 and RR42_RS12705 are separated by 28 nucleotides
RR42_RS12690: RR42_RS12690 - NC domain protein, at 2,723,500 to 2,723,976
_RS12690
RR42_RS12695: RR42_RS12695 - hypothetical protein, at 2,724,534 to 2,724,821
_RS12695
RR42_RS12700: RR42_RS12700 - serine/threonine phosphatase, at 2,724,937 to 2,725,707
_RS12700
RR42_RS12705: RR42_RS12705 - transcriptional regulator, at 2,725,736 to 2,727,043
_RS12705
Position (kb)
2724
2725
2726 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2723.944 kb on - strand at 2723.999 kb on - strand at 2724.038 kb on + strand at 2724.157 kb on - strand at 2724.418 kb on + strand at 2724.465 kb on + strand at 2724.466 kb on - strand at 2724.466 kb on - strand at 2724.467 kb on + strand at 2724.468 kb on - strand at 2724.468 kb on - strand at 2724.561 kb on + strand at 2724.562 kb on - strand at 2724.562 kb on - strand at 2724.562 kb on - strand at 2724.562 kb on - strand at 2724.645 kb on + strand, within RR42_RS12695 at 2724.708 kb on + strand, within RR42_RS12695 at 2724.708 kb on + strand, within RR42_RS12695 at 2724.709 kb on - strand, within RR42_RS12695 at 2724.755 kb on + strand, within RR42_RS12695 at 2725.098 kb on + strand, within RR42_RS12700 at 2725.099 kb on - strand, within RR42_RS12700 at 2725.099 kb on - strand, within RR42_RS12700 at 2725.146 kb on + strand, within RR42_RS12700 at 2725.248 kb on - strand, within RR42_RS12700 at 2725.306 kb on + strand, within RR42_RS12700 at 2725.306 kb on + strand, within RR42_RS12700 at 2725.306 kb on + strand, within RR42_RS12700 at 2725.307 kb on - strand, within RR42_RS12700 at 2725.670 kb on - strand at 2726.077 kb on + strand, within RR42_RS12705 at 2726.077 kb on + strand, within RR42_RS12705 at 2726.078 kb on - strand, within RR42_RS12705 at 2726.082 kb on + strand, within RR42_RS12705 at 2726.083 kb on - strand, within RR42_RS12705 at 2726.125 kb on - strand, within RR42_RS12705 at 2726.125 kb on - strand, within RR42_RS12705 at 2726.125 kb on - strand, within RR42_RS12705 at 2726.125 kb on - strand, within RR42_RS12705 at 2726.205 kb on + strand, within RR42_RS12705 at 2726.205 kb on + strand, within RR42_RS12705 at 2726.600 kb on - strand, within RR42_RS12705 at 2726.600 kb on - strand, within RR42_RS12705 at 2726.600 kb on - strand, within RR42_RS12705 at 2726.600 kb on - strand, within RR42_RS12705 at 2726.601 kb on + strand, within RR42_RS12705 at 2726.602 kb on - strand, within RR42_RS12705 at 2726.602 kb on - strand, within RR42_RS12705
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,723,944 - +0.0 2,723,999 - -3.0 2,724,038 + -1.2 2,724,157 - +0.3 2,724,418 + +1.1 2,724,465 + +1.4 2,724,466 - -1.0 2,724,466 - +0.5 2,724,467 + -1.1 2,724,468 - +1.7 2,724,468 - +0.4 2,724,561 + +0.1 2,724,562 - -0.4 2,724,562 - -1.4 2,724,562 - -0.1 2,724,562 - +0.5 2,724,645 + RR42_RS12695 0.39 +0.8 2,724,708 + RR42_RS12695 0.60 +0.7 2,724,708 + RR42_RS12695 0.60 -1.7 2,724,709 - RR42_RS12695 0.61 -0.8 2,724,755 + RR42_RS12695 0.77 -0.6 2,725,098 + RR42_RS12700 0.21 -0.0 2,725,099 - RR42_RS12700 0.21 +0.2 2,725,099 - RR42_RS12700 0.21 -0.7 2,725,146 + RR42_RS12700 0.27 -1.1 2,725,248 - RR42_RS12700 0.40 +1.9 2,725,306 + RR42_RS12700 0.48 +0.7 2,725,306 + RR42_RS12700 0.48 -1.4 2,725,306 + RR42_RS12700 0.48 +0.3 2,725,307 - RR42_RS12700 0.48 +2.2 2,725,670 - +1.3 2,726,077 + RR42_RS12705 0.26 +0.4 2,726,077 + RR42_RS12705 0.26 -1.2 2,726,078 - RR42_RS12705 0.26 -0.2 2,726,082 + RR42_RS12705 0.26 -0.1 2,726,083 - RR42_RS12705 0.27 -0.3 2,726,125 - RR42_RS12705 0.30 -0.4 2,726,125 - RR42_RS12705 0.30 +0.3 2,726,125 - RR42_RS12705 0.30 +0.1 2,726,125 - RR42_RS12705 0.30 +0.6 2,726,205 + RR42_RS12705 0.36 +1.8 2,726,205 + RR42_RS12705 0.36 -0.0 2,726,600 - RR42_RS12705 0.66 -0.9 2,726,600 - RR42_RS12705 0.66 +0.6 2,726,600 - RR42_RS12705 0.66 -1.5 2,726,600 - RR42_RS12705 0.66 +0.3 2,726,601 + RR42_RS12705 0.66 -0.6 2,726,602 - RR42_RS12705 0.66 -1.9 2,726,602 - RR42_RS12705 0.66 +1.9
Or see this region's nucleotide sequence