Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11995 and RR42_RS12000 are separated by 105 nucleotides RR42_RS12000 and RR42_RS12005 are separated by 5 nucleotides
RR42_RS11995: RR42_RS11995 - glutathione ABC transporter ATP-binding protein, at 2,570,029 to 2,571,918
_RS11995
RR42_RS12000: RR42_RS12000 - isoaspartyl peptidase, at 2,572,024 to 2,572,977
_RS12000
RR42_RS12005: RR42_RS12005 - transcriptional regulator, at 2,572,983 to 2,573,942
_RS12005
Position (kb)
2572
2573 Strain fitness (log2 ratio)
-2
-1
0
1 at 2571.114 kb on - strand, within RR42_RS11995 at 2571.176 kb on + strand, within RR42_RS11995 at 2571.177 kb on - strand, within RR42_RS11995 at 2571.177 kb on - strand, within RR42_RS11995 at 2571.545 kb on - strand, within RR42_RS11995 at 2571.930 kb on + strand at 2571.931 kb on - strand at 2571.938 kb on - strand at 2571.938 kb on - strand at 2571.938 kb on - strand at 2572.011 kb on + strand at 2572.032 kb on - strand at 2572.077 kb on - strand at 2572.077 kb on - strand at 2572.211 kb on + strand, within RR42_RS12000 at 2572.236 kb on - strand, within RR42_RS12000 at 2572.547 kb on + strand, within RR42_RS12000 at 2572.548 kb on - strand, within RR42_RS12000 at 2572.548 kb on - strand, within RR42_RS12000 at 2572.548 kb on - strand, within RR42_RS12000 at 2572.551 kb on - strand, within RR42_RS12000 at 2572.739 kb on + strand, within RR42_RS12000 at 2572.740 kb on - strand, within RR42_RS12000 at 2572.740 kb on - strand, within RR42_RS12000 at 2572.740 kb on - strand, within RR42_RS12000 at 2572.740 kb on - strand, within RR42_RS12000 at 2572.740 kb on - strand, within RR42_RS12000 at 2572.896 kb on - strand at 2573.174 kb on - strand, within RR42_RS12005 at 2573.202 kb on - strand, within RR42_RS12005 at 2573.258 kb on - strand, within RR42_RS12005 at 2573.365 kb on + strand, within RR42_RS12005 at 2573.365 kb on + strand, within RR42_RS12005 at 2573.365 kb on + strand, within RR42_RS12005 at 2573.366 kb on - strand, within RR42_RS12005 at 2573.366 kb on - strand, within RR42_RS12005 at 2573.366 kb on - strand, within RR42_RS12005 at 2573.371 kb on + strand, within RR42_RS12005 at 2573.372 kb on - strand, within RR42_RS12005 at 2573.486 kb on + strand, within RR42_RS12005 at 2573.487 kb on - strand, within RR42_RS12005 at 2573.878 kb on + strand at 2573.879 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,571,114 - RR42_RS11995 0.57 -0.6 2,571,176 + RR42_RS11995 0.61 +0.0 2,571,177 - RR42_RS11995 0.61 -0.8 2,571,177 - RR42_RS11995 0.61 -1.1 2,571,545 - RR42_RS11995 0.80 +1.5 2,571,930 + +0.2 2,571,931 - -0.3 2,571,938 - -1.2 2,571,938 - -2.0 2,571,938 - +0.3 2,572,011 + +1.3 2,572,032 - -0.6 2,572,077 - -0.2 2,572,077 - -0.9 2,572,211 + RR42_RS12000 0.20 -2.0 2,572,236 - RR42_RS12000 0.22 -2.3 2,572,547 + RR42_RS12000 0.55 -0.5 2,572,548 - RR42_RS12000 0.55 -2.2 2,572,548 - RR42_RS12000 0.55 -0.2 2,572,548 - RR42_RS12000 0.55 -1.5 2,572,551 - RR42_RS12000 0.55 -2.0 2,572,739 + RR42_RS12000 0.75 -0.2 2,572,740 - RR42_RS12000 0.75 -1.2 2,572,740 - RR42_RS12000 0.75 +0.1 2,572,740 - RR42_RS12000 0.75 +0.6 2,572,740 - RR42_RS12000 0.75 -1.5 2,572,740 - RR42_RS12000 0.75 -0.1 2,572,896 - +0.8 2,573,174 - RR42_RS12005 0.20 -1.0 2,573,202 - RR42_RS12005 0.23 -2.0 2,573,258 - RR42_RS12005 0.29 -0.7 2,573,365 + RR42_RS12005 0.40 +0.9 2,573,365 + RR42_RS12005 0.40 -0.8 2,573,365 + RR42_RS12005 0.40 -2.0 2,573,366 - RR42_RS12005 0.40 +0.1 2,573,366 - RR42_RS12005 0.40 -2.2 2,573,366 - RR42_RS12005 0.40 +0.2 2,573,371 + RR42_RS12005 0.40 -0.7 2,573,372 - RR42_RS12005 0.41 +1.2 2,573,486 + RR42_RS12005 0.52 -0.4 2,573,487 - RR42_RS12005 0.53 -0.8 2,573,878 + +0.3 2,573,879 - +0.8
Or see this region's nucleotide sequence