Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11975 and RR42_RS11980 overlap by 23 nucleotides RR42_RS11980 and RR42_RS11985 are separated by 11 nucleotides
RR42_RS11975: RR42_RS11975 - hypothetical protein, at 2,565,408 to 2,566,475
_RS11975
RR42_RS11980: RR42_RS11980 - glutathione ABC transporter permease, at 2,566,453 to 2,567,370
_RS11980
RR42_RS11985: RR42_RS11985 - glutathione ABC transporter permease, at 2,567,382 to 2,568,302
_RS11985
Position (kb)
2566
2567
2568 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2565.533 kb on + strand, within RR42_RS11975 at 2565.601 kb on + strand, within RR42_RS11975 at 2565.601 kb on + strand, within RR42_RS11975 at 2565.602 kb on - strand, within RR42_RS11975 at 2565.602 kb on - strand, within RR42_RS11975 at 2565.648 kb on + strand, within RR42_RS11975 at 2565.786 kb on - strand, within RR42_RS11975 at 2566.041 kb on + strand, within RR42_RS11975 at 2566.041 kb on + strand, within RR42_RS11975 at 2566.042 kb on - strand, within RR42_RS11975 at 2566.042 kb on - strand, within RR42_RS11975 at 2566.054 kb on + strand, within RR42_RS11975 at 2566.694 kb on + strand, within RR42_RS11980 at 2566.784 kb on + strand, within RR42_RS11980 at 2566.784 kb on + strand, within RR42_RS11980 at 2566.785 kb on - strand, within RR42_RS11980 at 2566.809 kb on + strand, within RR42_RS11980 at 2566.810 kb on - strand, within RR42_RS11980 at 2566.983 kb on - strand, within RR42_RS11980 at 2566.983 kb on - strand, within RR42_RS11980 at 2566.983 kb on - strand, within RR42_RS11980 at 2567.181 kb on - strand, within RR42_RS11980 at 2567.359 kb on + strand at 2567.384 kb on - strand at 2567.384 kb on - strand at 2567.384 kb on - strand at 2567.388 kb on - strand at 2567.389 kb on + strand at 2567.390 kb on - strand at 2567.785 kb on + strand, within RR42_RS11985 at 2567.785 kb on + strand, within RR42_RS11985 at 2567.785 kb on + strand, within RR42_RS11985 at 2567.786 kb on - strand, within RR42_RS11985 at 2567.799 kb on - strand, within RR42_RS11985 at 2568.318 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,565,533 + RR42_RS11975 0.12 +0.1 2,565,601 + RR42_RS11975 0.18 -1.2 2,565,601 + RR42_RS11975 0.18 -0.2 2,565,602 - RR42_RS11975 0.18 -0.6 2,565,602 - RR42_RS11975 0.18 -0.2 2,565,648 + RR42_RS11975 0.22 -1.1 2,565,786 - RR42_RS11975 0.35 -2.0 2,566,041 + RR42_RS11975 0.59 -0.7 2,566,041 + RR42_RS11975 0.59 +0.2 2,566,042 - RR42_RS11975 0.59 +0.3 2,566,042 - RR42_RS11975 0.59 +0.1 2,566,054 + RR42_RS11975 0.60 -1.3 2,566,694 + RR42_RS11980 0.26 -3.0 2,566,784 + RR42_RS11980 0.36 -0.6 2,566,784 + RR42_RS11980 0.36 +0.3 2,566,785 - RR42_RS11980 0.36 -1.7 2,566,809 + RR42_RS11980 0.39 +0.2 2,566,810 - RR42_RS11980 0.39 -4.4 2,566,983 - RR42_RS11980 0.58 +1.1 2,566,983 - RR42_RS11980 0.58 -1.8 2,566,983 - RR42_RS11980 0.58 -0.8 2,567,181 - RR42_RS11980 0.79 -0.2 2,567,359 + +0.8 2,567,384 - +0.8 2,567,384 - -1.3 2,567,384 - -3.4 2,567,388 - +0.7 2,567,389 + -2.1 2,567,390 - +1.1 2,567,785 + RR42_RS11985 0.44 +1.0 2,567,785 + RR42_RS11985 0.44 +1.4 2,567,785 + RR42_RS11985 0.44 +1.4 2,567,786 - RR42_RS11985 0.44 +0.4 2,567,799 - RR42_RS11985 0.45 -1.4 2,568,318 + +0.3
Or see this region's nucleotide sequence