Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11895 and RR42_RS11900 are separated by 122 nucleotides RR42_RS11900 and RR42_RS11905 overlap by 4 nucleotides
RR42_RS11895: RR42_RS11895 - membrane protein, at 2,545,606 to 2,546,760
_RS11895
RR42_RS11900: RR42_RS11900 - peptide deformylase, at 2,546,883 to 2,547,416
_RS11900
RR42_RS11905: RR42_RS11905 - NAD-dependent DNA ligase LigA, at 2,547,413 to 2,549,539
_RS11905
Position (kb)
2546
2547
2548 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2545.908 kb on - strand, within RR42_RS11895 at 2545.908 kb on - strand, within RR42_RS11895 at 2545.929 kb on - strand, within RR42_RS11895 at 2545.968 kb on - strand, within RR42_RS11895 at 2545.998 kb on - strand, within RR42_RS11895 at 2546.001 kb on + strand, within RR42_RS11895 at 2546.001 kb on + strand, within RR42_RS11895 at 2546.001 kb on + strand, within RR42_RS11895 at 2546.001 kb on + strand, within RR42_RS11895 at 2546.006 kb on + strand, within RR42_RS11895 at 2546.007 kb on - strand, within RR42_RS11895 at 2546.072 kb on + strand, within RR42_RS11895 at 2546.078 kb on + strand, within RR42_RS11895 at 2546.078 kb on + strand, within RR42_RS11895 at 2546.079 kb on - strand, within RR42_RS11895 at 2546.079 kb on - strand, within RR42_RS11895 at 2546.079 kb on - strand, within RR42_RS11895 at 2546.103 kb on - strand, within RR42_RS11895 at 2546.159 kb on + strand, within RR42_RS11895 at 2546.159 kb on + strand, within RR42_RS11895 at 2546.159 kb on + strand, within RR42_RS11895 at 2546.160 kb on - strand, within RR42_RS11895 at 2546.160 kb on - strand, within RR42_RS11895 at 2546.462 kb on + strand, within RR42_RS11895 at 2546.462 kb on + strand, within RR42_RS11895 at 2546.463 kb on - strand, within RR42_RS11895 at 2546.565 kb on - strand, within RR42_RS11895 at 2546.565 kb on - strand, within RR42_RS11895 at 2546.760 kb on + strand at 2546.761 kb on - strand at 2546.927 kb on + strand at 2546.928 kb on - strand at 2547.067 kb on + strand, within RR42_RS11900 at 2547.314 kb on + strand, within RR42_RS11900 at 2547.314 kb on + strand, within RR42_RS11900 at 2547.315 kb on - strand, within RR42_RS11900 at 2547.433 kb on + strand at 2547.433 kb on + strand at 2547.434 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,545,908 - RR42_RS11895 0.26 +0.4 2,545,908 - RR42_RS11895 0.26 -0.6 2,545,929 - RR42_RS11895 0.28 +0.4 2,545,968 - RR42_RS11895 0.31 -0.9 2,545,998 - RR42_RS11895 0.34 +1.1 2,546,001 + RR42_RS11895 0.34 -1.4 2,546,001 + RR42_RS11895 0.34 -2.0 2,546,001 + RR42_RS11895 0.34 +1.0 2,546,001 + RR42_RS11895 0.34 +0.0 2,546,006 + RR42_RS11895 0.35 -1.3 2,546,007 - RR42_RS11895 0.35 +0.4 2,546,072 + RR42_RS11895 0.40 +0.2 2,546,078 + RR42_RS11895 0.41 -0.1 2,546,078 + RR42_RS11895 0.41 +0.6 2,546,079 - RR42_RS11895 0.41 -0.6 2,546,079 - RR42_RS11895 0.41 -2.8 2,546,079 - RR42_RS11895 0.41 -0.2 2,546,103 - RR42_RS11895 0.43 -1.6 2,546,159 + RR42_RS11895 0.48 -2.1 2,546,159 + RR42_RS11895 0.48 +0.2 2,546,159 + RR42_RS11895 0.48 -0.8 2,546,160 - RR42_RS11895 0.48 -1.4 2,546,160 - RR42_RS11895 0.48 +0.0 2,546,462 + RR42_RS11895 0.74 -1.5 2,546,462 + RR42_RS11895 0.74 +1.2 2,546,463 - RR42_RS11895 0.74 +0.3 2,546,565 - RR42_RS11895 0.83 +2.2 2,546,565 - RR42_RS11895 0.83 +2.1 2,546,760 + -0.3 2,546,761 - -0.0 2,546,927 + +0.5 2,546,928 - +0.6 2,547,067 + RR42_RS11900 0.34 +0.6 2,547,314 + RR42_RS11900 0.81 +0.4 2,547,314 + RR42_RS11900 0.81 +1.1 2,547,315 - RR42_RS11900 0.81 -0.2 2,547,433 + +3.1 2,547,433 + +0.5 2,547,434 - +0.9
Or see this region's nucleotide sequence