Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11790 and RR42_RS11795 are separated by 364 nucleotides RR42_RS11795 and RR42_RS11800 are separated by 85 nucleotides RR42_RS11800 and RR42_RS11805 overlap by 4 nucleotides
RR42_RS11790: RR42_RS11790 - phosphoenolpyruvate synthase, at 2,519,787 to 2,522,171
_RS11790
RR42_RS11795: RR42_RS11795 - PEP synthetase regulatory protein, at 2,522,536 to 2,523,351
_RS11795
RR42_RS11800: RR42_RS11800 - RNA methyltransferase, at 2,523,437 to 2,524,219
_RS11800
RR42_RS11805: RR42_RS11805 - ribonuclease HII, at 2,524,216 to 2,524,926
_RS11805
Position (kb)
2522
2523
2524 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2521.643 kb on - strand, within RR42_RS11790 at 2521.663 kb on + strand, within RR42_RS11790 at 2521.663 kb on + strand, within RR42_RS11790 at 2521.663 kb on + strand, within RR42_RS11790 at 2521.663 kb on + strand, within RR42_RS11790 at 2521.664 kb on - strand, within RR42_RS11790 at 2521.726 kb on + strand, within RR42_RS11790 at 2522.144 kb on - strand at 2522.144 kb on - strand at 2522.171 kb on + strand at 2522.190 kb on + strand at 2522.190 kb on + strand at 2522.191 kb on - strand at 2522.217 kb on + strand at 2522.217 kb on + strand at 2522.254 kb on + strand at 2522.385 kb on + strand at 2522.437 kb on + strand at 2522.437 kb on + strand at 2522.550 kb on + strand at 2522.550 kb on + strand at 2522.550 kb on + strand at 2522.701 kb on + strand, within RR42_RS11795 at 2522.701 kb on + strand, within RR42_RS11795 at 2523.020 kb on - strand, within RR42_RS11795 at 2523.346 kb on + strand at 2523.346 kb on + strand at 2523.346 kb on + strand at 2523.346 kb on + strand at 2523.347 kb on - strand at 2523.347 kb on - strand at 2523.832 kb on + strand, within RR42_RS11800 at 2523.832 kb on + strand, within RR42_RS11800 at 2523.889 kb on + strand, within RR42_RS11800 at 2524.045 kb on - strand, within RR42_RS11800 at 2524.045 kb on - strand, within RR42_RS11800 at 2524.045 kb on - strand, within RR42_RS11800 at 2524.045 kb on - strand, within RR42_RS11800 at 2524.045 kb on - strand, within RR42_RS11800 at 2524.045 kb on - strand, within RR42_RS11800 at 2524.095 kb on + strand, within RR42_RS11800 at 2524.095 kb on + strand, within RR42_RS11800 at 2524.095 kb on + strand, within RR42_RS11800 at 2524.096 kb on - strand, within RR42_RS11800 at 2524.096 kb on - strand, within RR42_RS11800
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,521,643 - RR42_RS11790 0.78 -1.4 2,521,663 + RR42_RS11790 0.79 +2.4 2,521,663 + RR42_RS11790 0.79 -0.2 2,521,663 + RR42_RS11790 0.79 -1.7 2,521,663 + RR42_RS11790 0.79 -0.1 2,521,664 - RR42_RS11790 0.79 -1.5 2,521,726 + RR42_RS11790 0.81 -3.3 2,522,144 - -1.8 2,522,144 - -3.0 2,522,171 + -3.5 2,522,190 + -2.7 2,522,190 + -2.9 2,522,191 - -0.2 2,522,217 + -0.2 2,522,217 + -2.4 2,522,254 + -3.2 2,522,385 + -1.2 2,522,437 + +0.0 2,522,437 + -2.4 2,522,550 + -1.5 2,522,550 + +0.8 2,522,550 + -0.1 2,522,701 + RR42_RS11795 0.20 -1.7 2,522,701 + RR42_RS11795 0.20 -1.2 2,523,020 - RR42_RS11795 0.59 -0.8 2,523,346 + +1.1 2,523,346 + +0.1 2,523,346 + -1.7 2,523,346 + +0.0 2,523,347 - -1.0 2,523,347 - +0.3 2,523,832 + RR42_RS11800 0.50 -0.2 2,523,832 + RR42_RS11800 0.50 -1.0 2,523,889 + RR42_RS11800 0.58 +1.5 2,524,045 - RR42_RS11800 0.78 +1.0 2,524,045 - RR42_RS11800 0.78 +1.2 2,524,045 - RR42_RS11800 0.78 -2.4 2,524,045 - RR42_RS11800 0.78 -0.1 2,524,045 - RR42_RS11800 0.78 -0.2 2,524,045 - RR42_RS11800 0.78 -0.6 2,524,095 + RR42_RS11800 0.84 -0.4 2,524,095 + RR42_RS11800 0.84 +0.1 2,524,095 + RR42_RS11800 0.84 +1.0 2,524,096 - RR42_RS11800 0.84 -1.3 2,524,096 - RR42_RS11800 0.84 -1.3
Or see this region's nucleotide sequence