Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11615 and RR42_RS11620 are separated by 110 nucleotides RR42_RS11620 and RR42_RS11625 are separated by 152 nucleotides
RR42_RS11615: RR42_RS11615 - GTP-binding protein, at 2,486,971 to 2,488,245
_RS11615
RR42_RS11620: RR42_RS11620 - polysaccharide deacetylase, at 2,488,356 to 2,489,111
_RS11620
RR42_RS11625: RR42_RS11625 - hypothetical protein, at 2,489,264 to 2,490,613
_RS11625
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2487.374 kb on + strand, within RR42_RS11615 at 2487.488 kb on + strand, within RR42_RS11615 at 2487.853 kb on - strand, within RR42_RS11615 at 2487.927 kb on - strand, within RR42_RS11615 at 2488.005 kb on - strand, within RR42_RS11615 at 2488.101 kb on + strand, within RR42_RS11615 at 2488.109 kb on - strand, within RR42_RS11615 at 2488.292 kb on + strand at 2488.292 kb on + strand at 2488.292 kb on + strand at 2488.293 kb on - strand at 2488.510 kb on + strand, within RR42_RS11620 at 2488.729 kb on + strand, within RR42_RS11620 at 2488.760 kb on + strand, within RR42_RS11620 at 2488.761 kb on - strand, within RR42_RS11620 at 2488.761 kb on - strand, within RR42_RS11620 at 2489.011 kb on + strand, within RR42_RS11620 at 2489.011 kb on + strand, within RR42_RS11620 at 2489.012 kb on - strand, within RR42_RS11620 at 2489.092 kb on - strand at 2489.117 kb on - strand at 2489.245 kb on - strand at 2489.250 kb on + strand at 2489.265 kb on + strand at 2489.266 kb on - strand at 2489.358 kb on - strand at 2489.669 kb on + strand, within RR42_RS11625 at 2489.669 kb on + strand, within RR42_RS11625 at 2489.670 kb on - strand, within RR42_RS11625 at 2489.796 kb on + strand, within RR42_RS11625 at 2489.796 kb on + strand, within RR42_RS11625 at 2489.797 kb on - strand, within RR42_RS11625 at 2489.797 kb on - strand, within RR42_RS11625 at 2489.837 kb on + strand, within RR42_RS11625 at 2489.837 kb on + strand, within RR42_RS11625 at 2489.838 kb on - strand, within RR42_RS11625 at 2489.838 kb on - strand, within RR42_RS11625 at 2489.856 kb on - strand, within RR42_RS11625 at 2490.031 kb on - strand, within RR42_RS11625 at 2490.031 kb on - strand, within RR42_RS11625 at 2490.074 kb on + strand, within RR42_RS11625 at 2490.074 kb on + strand, within RR42_RS11625 at 2490.075 kb on - strand, within RR42_RS11625 at 2490.076 kb on - strand, within RR42_RS11625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,487,374 + RR42_RS11615 0.32 -0.2 2,487,488 + RR42_RS11615 0.41 +0.9 2,487,853 - RR42_RS11615 0.69 -0.8 2,487,927 - RR42_RS11615 0.75 -2.2 2,488,005 - RR42_RS11615 0.81 -1.4 2,488,101 + RR42_RS11615 0.89 -0.7 2,488,109 - RR42_RS11615 0.89 +0.2 2,488,292 + +0.4 2,488,292 + -0.8 2,488,292 + +0.2 2,488,293 - +0.4 2,488,510 + RR42_RS11620 0.20 -1.7 2,488,729 + RR42_RS11620 0.49 +0.4 2,488,760 + RR42_RS11620 0.53 -0.3 2,488,761 - RR42_RS11620 0.54 -0.6 2,488,761 - RR42_RS11620 0.54 +0.2 2,489,011 + RR42_RS11620 0.87 -2.2 2,489,011 + RR42_RS11620 0.87 +0.7 2,489,012 - RR42_RS11620 0.87 -1.8 2,489,092 - +0.8 2,489,117 - +0.2 2,489,245 - -0.3 2,489,250 + -0.6 2,489,265 + +2.0 2,489,266 - -0.2 2,489,358 - +0.3 2,489,669 + RR42_RS11625 0.30 +2.0 2,489,669 + RR42_RS11625 0.30 +0.9 2,489,670 - RR42_RS11625 0.30 -1.7 2,489,796 + RR42_RS11625 0.39 +0.4 2,489,796 + RR42_RS11625 0.39 +0.2 2,489,797 - RR42_RS11625 0.39 +0.3 2,489,797 - RR42_RS11625 0.39 -0.4 2,489,837 + RR42_RS11625 0.42 +0.4 2,489,837 + RR42_RS11625 0.42 -0.6 2,489,838 - RR42_RS11625 0.43 -2.0 2,489,838 - RR42_RS11625 0.43 +1.4 2,489,856 - RR42_RS11625 0.44 -0.7 2,490,031 - RR42_RS11625 0.57 -0.9 2,490,031 - RR42_RS11625 0.57 +0.1 2,490,074 + RR42_RS11625 0.60 +0.2 2,490,074 + RR42_RS11625 0.60 +0.3 2,490,075 - RR42_RS11625 0.60 -1.2 2,490,076 - RR42_RS11625 0.60 +0.0
Or see this region's nucleotide sequence