Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11375 and RR42_RS11380 are separated by 113 nucleotides RR42_RS11380 and RR42_RS11385 are separated by 76 nucleotides RR42_RS11385 and RR42_RS11390 are separated by 67 nucleotides RR42_RS11390 and RR42_RS11395 are separated by 250 nucleotides
RR42_RS11375: RR42_RS11375 - peptidase S66, at 2,436,600 to 2,437,574
_RS11375
RR42_RS11380: RR42_RS11380 - hypothetical protein, at 2,437,688 to 2,438,197
_RS11380
RR42_RS11385: RR42_RS11385 - 6-carboxy-5,6,7,8-tetrahydropterin synthase, at 2,438,274 to 2,438,750
_RS11385
RR42_RS11390: RR42_RS11390 - 7-cyano-7-deazaguanine reductase, at 2,438,818 to 2,439,465
_RS11390
RR42_RS11395: RR42_RS11395 - LysR family transcriptional regulator, at 2,439,716 to 2,440,621
_RS11395
Position (kb)
2438
2439 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2437.673 kb on + strand at 2437.674 kb on - strand at 2437.685 kb on + strand at 2437.685 kb on + strand at 2437.686 kb on - strand at 2437.686 kb on - strand at 2437.686 kb on - strand at 2438.167 kb on - strand at 2438.794 kb on - strand at 2438.967 kb on - strand, within RR42_RS11390 at 2439.276 kb on - strand, within RR42_RS11390 at 2439.437 kb on + strand at 2439.458 kb on + strand at 2439.476 kb on - strand at 2439.487 kb on - strand at 2439.493 kb on + strand at 2439.494 kb on - strand at 2439.494 kb on - strand at 2439.494 kb on - strand at 2439.494 kb on - strand at 2439.495 kb on + strand at 2439.495 kb on + strand at 2439.495 kb on + strand at 2439.496 kb on - strand at 2439.496 kb on - strand at 2439.671 kb on + strand at 2439.672 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,437,673 + -1.6 2,437,674 - -3.6 2,437,685 + -2.1 2,437,685 + -1.5 2,437,686 - +1.0 2,437,686 - +1.1 2,437,686 - +0.4 2,438,167 - -2.6 2,438,794 - -1.3 2,438,967 - RR42_RS11390 0.23 +0.5 2,439,276 - RR42_RS11390 0.71 -0.2 2,439,437 + -0.9 2,439,458 + -1.5 2,439,476 - -0.1 2,439,487 - -3.5 2,439,493 + -3.1 2,439,494 - -0.7 2,439,494 - -2.9 2,439,494 - +0.8 2,439,494 - +0.2 2,439,495 + -0.0 2,439,495 + -1.2 2,439,495 + +0.2 2,439,496 - +0.2 2,439,496 - +0.6 2,439,671 + -0.9 2,439,672 - -1.2
Or see this region's nucleotide sequence